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Title: A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry

Abstract

Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes offers multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. In this work, we discuss a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.

Authors:
Publication Date:
Research Org.:
Princeton Univ., NJ (United States); Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); European Research Council (ERC); German Research Foundation (DFG)
OSTI Identifier:
1632296
Alternate Identifier(s):
OSTI ID: 1421290
Grant/Contract Number:  
SC0018260
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Angewandte Chemie (International Edition)
Additional Journal Information:
Journal Volume: 57; Journal Issue: 11; Journal ID: ISSN 1433-7851
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; complementary ion clusters; isobaric labelling; mass spectrometry; peptides; proteomics; sulfoxide pyrolysis

Citation Formats

None, None. A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry. United States: N. p., 2018. Web. doi:10.1002/anie.201708867.
None, None. A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry. United States. doi:10.1002/anie.201708867.
None, None. Mon . "A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry". United States. doi:10.1002/anie.201708867. https://www.osti.gov/servlets/purl/1632296.
@article{osti_1632296,
title = {A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry},
author = {None, None},
abstractNote = {Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes offers multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. In this work, we discuss a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.},
doi = {10.1002/anie.201708867},
journal = {Angewandte Chemie (International Edition)},
issn = {1433-7851},
number = 11,
volume = 57,
place = {United States},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 4 works
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