Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis

Tsogtbaatar, Enkhtuul; Cocuron, Jean-Christophe; Alonso, Ana Paula; Lunn, ed., John

doi:10.1093/jxb/eraa060

Title: Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis

Journal Article · Sat Feb 01 00:00:00 EST 2020 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/eraa060· OSTI ID:1631345

Tsogtbaatar, Enkhtuul ^[1]; Cocuron, Jean-Christophe ^[2];

^[3]; Lunn, ed., John ^[4]

Ohio State Biochemistry Program, The Ohio State University, Columbus, OH, USA
BioDiscovery Institute, University of North Texas, Denton, TX, USA
BioDiscovery Institute, University of North Texas, Denton, TX, USA, Department of Biological Sciences, University of North Texas, Denton, TX, USA
MPI of Molecular Plant Physiology, Germany

Abstract Pennycress (Thlaspi arvense L.) accumulates oil up to 35% of the total seed biomass, and its overall fatty acid composition is suitable for aviation fuel. However, for this plant to become economically viable, its oil production needs to be improved. In vivo culture conditions that resemble the development of pennycress embryos in planta were developed based on the composition of the liquid endosperm. Then, substrate uptake rates and biomass accumulation were measured from cultured pennycress embryos, revealing a biosynthetic efficiency of 93%, which is one of the highest in comparison with other oilseeds to date. Additionally, the ratio of carbon in oil to CO2 indicated that non-conventional pathways are likely to be responsible for such a high carbon conversion efficiency. To identify the reactions enabling this phenomenon, parallel labeling experiments with 13C-labeled substrates were conducted in pennycress embryos. The main findings of these labeling experiments include: (i) the occurrence of the oxidative reactions of the pentose phosphate pathway in the cytosol; (ii) the reversibility of isocitrate dehydrogenase; (iii) the operation of the plastidic NADP-dependent malic enzyme; and (iv) the refixation of CO2 by Rubisco. These reactions are key providers of carbon and reductant for fatty acid synthesis and elongation.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019233

OSTI ID:: 1631345

Alternate ID(s):: OSTI ID: 1605939; OSTI ID: 1658579

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Vol. 71 Journal Issue: 10; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 23 works

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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
13C-labeling
carbon conversion efficiency
isocitrate dehydrogenase
oilseed
pennycress
plant metabolism
RuBisCO

Title: Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis

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