Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions

Blachowicz, Adriana; Chiang, Abby J.; Elsaesser, Andreas; Kalkum, Markus; Ehrenfreund, Pascale; Stajich, Jason E.; Torok, Tamas; Wang, Clay C.  C.; Venkateswaran, Kasthuri

doi:10.3389/fmicb.2019.01013

Title: Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions

Journal Article · Wed May 15 00:00:00 EDT 2019 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2019.01013· OSTI ID:1628176

Blachowicz, Adriana ^[1]; Chiang, Abby J. ^[2]; Elsaesser, Andreas ^[3]; Kalkum, Markus ^[2]; Ehrenfreund, Pascale ^[4]; Stajich, Jason E. ^[5]; Torok, Tamas ^[6]; Wang, Clay C. C. ^[7]; Venkateswaran, Kasthuri ^[8]

Univ. of Southern California, Los Angeles, CA (United States). Dept. of Pharmacology and Pharmaceutical Sciences; California Institute of Technology (CalTech), Pasadena, CA (United States). Jet Propulsion Lab. (JPL). Biotechnology and Planetary Protection Group
Beckman Research Inst. of City of Hope, Duarte, CA (United States). Dept. of Molecular Imaging and Therapy
Freie Univ., Berlin (Germany). Dept. of Physics
Leiden Univ. (Netherlands). Leiden Inst. of Chemistry
Univ. of California, Riverside, CA (United States). Inst. of Integrative Genome Biology. Dept. of Microbiology and Plant Pathology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Dept. of Ecology
Univ. of Southern California, Los Angeles, CA (United States). Dept. of Pharmacology and Pharmaceutical Sciences; Univ. of Southern California, Los Angeles, CA (United States). Dornsife College of Letters, Arts, and Sciences. Dept. of Chemistry
California Institute of Technology (CalTech), Pasadena, CA (United States). Jet Propulsion Lab. (JPL). Biotechnology and Planetary Protection Group

Filamentous fungi have been associated with extreme habitats, including nuclear power plant accident sites and the International Space Station (ISS). Due to their immense adaptation and phenotypic plasticity capacities, fungi may thrive in what seems like uninhabitable niches. This study is the first report of fungal survival after exposure of monolayers of conidia to simulated Mars conditions (SMC). Conidia of several Chernobyl nuclear accident-associated and ISS-isolated strains were tested for UV-C and SMC sensitivity, which resulted in strain-dependent survival. Strains surviving exposure to SMC for 30 min, ISSFT-021-30 and IMV 00236-30, were further characterized for proteomic, and metabolomic changes. Differential expression of proteins involved in ribosome biogenesis, translation, and carbohydrate metabolic processes was observed. No significant metabolome alterations were revealed. Lastly, ISSFT-021-30 conidia reexposed to UV-C exhibited enhanced UV-C resistance when compared to the conidia of unexposed ISSFT-021.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1628176

Journal Information:: Frontiers in Microbiology, Vol. 10; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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