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Title: Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions

Abstract

Filamentous fungi have been associated with extreme habitats, including nuclear power plant accident sites and the International Space Station (ISS). Due to their immense adaptation and phenotypic plasticity capacities, fungi may thrive in what seems like uninhabitable niches. This study is the first report of fungal survival after exposure of monolayers of conidia to simulated Mars conditions (SMC). Conidia of several Chernobyl nuclear accident-associated and ISS-isolated strains were tested for UV-C and SMC sensitivity, which resulted in strain-dependent survival. Strains surviving exposure to SMC for 30 min, ISSFT-021-30 and IMV 00236-30, were further characterized for proteomic, and metabolomic changes. Differential expression of proteins involved in ribosome biogenesis, translation, and carbohydrate metabolic processes was observed. No significant metabolome alterations were revealed. Lastly, ISSFT-021-30 conidia reexposed to UV-C exhibited enhanced UV-C resistance when compared to the conidia of unexposed ISSFT-021.

Authors:
 [1];  [2];  [3];  [2];  [4];  [5];  [6];  [7];  [8]
  1. Univ. of Southern California, Los Angeles, CA (United States). Dept. of Pharmacology and Pharmaceutical Sciences; California Institute of Technology (CalTech), Pasadena, CA (United States). Jet Propulsion Lab. (JPL). Biotechnology and Planetary Protection Group
  2. Beckman Research Inst. of City of Hope, Duarte, CA (United States). Dept. of Molecular Imaging and Therapy
  3. Freie Univ., Berlin (Germany). Dept. of Physics
  4. Leiden Univ. (Netherlands). Leiden Inst. of Chemistry
  5. Univ. of California, Riverside, CA (United States). Inst. of Integrative Genome Biology. Dept. of Microbiology and Plant Pathology
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Dept. of Ecology
  7. Univ. of Southern California, Los Angeles, CA (United States). Dept. of Pharmacology and Pharmaceutical Sciences; Univ. of Southern California, Los Angeles, CA (United States). Dornsife College of Letters, Arts, and Sciences. Dept. of Chemistry
  8. California Institute of Technology (CalTech), Pasadena, CA (United States). Jet Propulsion Lab. (JPL). Biotechnology and Planetary Protection Group
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1628176
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Microbiology; fungi; simulated mars conditions; proteome; metabolome; Chernobyl; international space station; extremophiles

Citation Formats

Blachowicz, Adriana, Chiang, Abby J., Elsaesser, Andreas, Kalkum, Markus, Ehrenfreund, Pascale, Stajich, Jason E., Torok, Tamas, Wang, Clay C. C., and Venkateswaran, Kasthuri. Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions. United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.01013.
Blachowicz, Adriana, Chiang, Abby J., Elsaesser, Andreas, Kalkum, Markus, Ehrenfreund, Pascale, Stajich, Jason E., Torok, Tamas, Wang, Clay C. C., & Venkateswaran, Kasthuri. Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions. United States. https://doi.org/10.3389/fmicb.2019.01013
Blachowicz, Adriana, Chiang, Abby J., Elsaesser, Andreas, Kalkum, Markus, Ehrenfreund, Pascale, Stajich, Jason E., Torok, Tamas, Wang, Clay C. C., and Venkateswaran, Kasthuri. 2019. "Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions". United States. https://doi.org/10.3389/fmicb.2019.01013. https://www.osti.gov/servlets/purl/1628176.
@article{osti_1628176,
title = {Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions},
author = {Blachowicz, Adriana and Chiang, Abby J. and Elsaesser, Andreas and Kalkum, Markus and Ehrenfreund, Pascale and Stajich, Jason E. and Torok, Tamas and Wang, Clay C. C. and Venkateswaran, Kasthuri},
abstractNote = {Filamentous fungi have been associated with extreme habitats, including nuclear power plant accident sites and the International Space Station (ISS). Due to their immense adaptation and phenotypic plasticity capacities, fungi may thrive in what seems like uninhabitable niches. This study is the first report of fungal survival after exposure of monolayers of conidia to simulated Mars conditions (SMC). Conidia of several Chernobyl nuclear accident-associated and ISS-isolated strains were tested for UV-C and SMC sensitivity, which resulted in strain-dependent survival. Strains surviving exposure to SMC for 30 min, ISSFT-021-30 and IMV 00236-30, were further characterized for proteomic, and metabolomic changes. Differential expression of proteins involved in ribosome biogenesis, translation, and carbohydrate metabolic processes was observed. No significant metabolome alterations were revealed. Lastly, ISSFT-021-30 conidia reexposed to UV-C exhibited enhanced UV-C resistance when compared to the conidia of unexposed ISSFT-021.},
doi = {10.3389/fmicb.2019.01013},
url = {https://www.osti.gov/biblio/1628176}, journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = ,
volume = 10,
place = {United States},
year = {Wed May 15 00:00:00 EDT 2019},
month = {Wed May 15 00:00:00 EDT 2019}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Figures / Tables:

FIGURE 1 FIGURE 1: UV-C resistance of Chernobyl and ISS-isolated fungal strains. Purified conidia of 13 strains were exposed to various UV-C doses. The UV-C survival rates were calculated using formula: N/N0, # of conidia survived at any given dose/# of conidia exposed at Time 0.

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An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota
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