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Title: Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals

Abstract

Enzymes from extremely thermophilic microorganisms have been of technological interest for some time because of their ability to catalyze reactions of industrial significance at elevated temperatures. Thermophilic enzymes are now routinely produced in recombinant mesophilic hosts for use as discrete biocatalysts. Genome and metagenome sequence data for extreme thermophiles provide useful information for putative biocatalysts for a wide range of biotransformations, albeit involving at most a few enzymatic steps. However, in the past several years, unprecedented progress has been made in establishing molecular genetics tools for extreme thermophiles to the point that the use of these microorganisms as metabolic engineering platforms has become possible. While in its early days, complex metabolic pathways have been altered or engineered into recombinant extreme thermophiles, such that the production of fuels and chemicals at elevated temperatures has become possible. Not only does this expand the thermal range for industrial biotechnology, it also potentially provides biodiverse options for specific biotransformations unique to these microorganisms. The list of extreme thermophiles growing optimally between 70 and 100°C with genetic toolkits currently available includes archaea and bacteria, aerobes and anaerobes, coming from genera such as Caldicellulosiruptor, Sulfolobus, Thermotoga, Thermococcus, and Pyrococcus. These organisms exhibit unusual and potentially usefulmore » native metabolic capabilities, including cellulose degradation, metal solubilization, and RuBisCO-free carbon fixation. Those looking to design a thermal bioprocess now have a host of potential candidates to choose from, each with its own advantages and challenges that will influence its appropriateness for specific applications. Here, the issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology.« less

Authors:
 [1];  [2];  [1];  [1];  [2];  [1]
  1. North Carolina State Univ., Raleigh, NC (United States). Dept. of Chemical and Biomolecular Engineering
  2. Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1628140
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Microbiology

Citation Formats

Zeldes, Benjamin M., Keller, Matthew W., Loder, Andrew J., Straub, Christopher T., Adams, Michael W. W., and Kelly, Robert M.. Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals. United States: N. p., 2015. Web. doi:10.3389/fmicb.2015.01209.
Zeldes, Benjamin M., Keller, Matthew W., Loder, Andrew J., Straub, Christopher T., Adams, Michael W. W., & Kelly, Robert M.. Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals. United States. https://doi.org/10.3389/fmicb.2015.01209
Zeldes, Benjamin M., Keller, Matthew W., Loder, Andrew J., Straub, Christopher T., Adams, Michael W. W., and Kelly, Robert M.. 2015. "Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals". United States. https://doi.org/10.3389/fmicb.2015.01209. https://www.osti.gov/servlets/purl/1628140.
@article{osti_1628140,
title = {Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals},
author = {Zeldes, Benjamin M. and Keller, Matthew W. and Loder, Andrew J. and Straub, Christopher T. and Adams, Michael W. W. and Kelly, Robert M.},
abstractNote = {Enzymes from extremely thermophilic microorganisms have been of technological interest for some time because of their ability to catalyze reactions of industrial significance at elevated temperatures. Thermophilic enzymes are now routinely produced in recombinant mesophilic hosts for use as discrete biocatalysts. Genome and metagenome sequence data for extreme thermophiles provide useful information for putative biocatalysts for a wide range of biotransformations, albeit involving at most a few enzymatic steps. However, in the past several years, unprecedented progress has been made in establishing molecular genetics tools for extreme thermophiles to the point that the use of these microorganisms as metabolic engineering platforms has become possible. While in its early days, complex metabolic pathways have been altered or engineered into recombinant extreme thermophiles, such that the production of fuels and chemicals at elevated temperatures has become possible. Not only does this expand the thermal range for industrial biotechnology, it also potentially provides biodiverse options for specific biotransformations unique to these microorganisms. The list of extreme thermophiles growing optimally between 70 and 100°C with genetic toolkits currently available includes archaea and bacteria, aerobes and anaerobes, coming from genera such as Caldicellulosiruptor, Sulfolobus, Thermotoga, Thermococcus, and Pyrococcus. These organisms exhibit unusual and potentially useful native metabolic capabilities, including cellulose degradation, metal solubilization, and RuBisCO-free carbon fixation. Those looking to design a thermal bioprocess now have a host of potential candidates to choose from, each with its own advantages and challenges that will influence its appropriateness for specific applications. Here, the issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology.},
doi = {10.3389/fmicb.2015.01209},
url = {https://www.osti.gov/biblio/1628140}, journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = ,
volume = 6,
place = {United States},
year = {2015},
month = {11}
}

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