Genomic Dissection of Anthracnose ( Colletotrichum sublineolum ) Resistance Response in Sorghum Differential Line SC112-14

Cruet-Burgos, Clara M.; Cuevas, Hugo E.; Prom, Louis K.; Knoll, Joseph E.; Stutts, Lauren R.; Vermerris, Wilfred

doi:10.1534/g3.120.401121

Title: Genomic Dissection of Anthracnose ( Colletotrichum sublineolum ) Resistance Response in Sorghum Differential Line SC112-14

Journal Article · Wed Feb 26 00:00:00 EST 2020 · G3

DOI:https://doi.org/10.1534/g3.120.401121· OSTI ID:1627963

Cruet-Burgos, Clara M. ^[1];

^[2]; Prom, Louis K. ^[3];

^[4]; Stutts, Lauren R. ^[5];

^[6]

USDA-ARS, Mayaguez (Puerto Rico). Tropical Agriculture Research Station; Univ. of Puerto Rico, Mayaguez (Puerto Rico). Dept. of Biology
USDA-ARS, Mayaguez (Puerto Rico). Tropical Agriculture Research Station; USDA-ARS, College Station, TX (United States). Southern Plains Agriculture Research Center
USDA-ARS, College Station, TX (United States). Southern Plains Agriculture Research Center
USDA-ARS, Tifton, GA (United States). Crop Genetics and Breeding Research
Univ. of Florida, Gainesville, FL (United States). Graduate Program in Plant and Molecular & Cellular Biology
Univ. of Florida, Gainesville, FL (United States). Genetics Inst., Dept. of Microbiology & Cell Science; Univ. of Florida, Gainesville, FL (United States). Florida Center for Renewable Fuels and Chemicals

Sorghum production is expanding to warmer and more humid regions where its production is being limited by multiple fungal pathogens. Anthracnose, caused byColletotrichum sublineolum, is one of the major diseases in these regions, where it can cause yield losses of both grain and biomass. In this study, 114 recombinant inbred lines (RILs) derived from resistant sorghum line SC112-14 were evaluated at four distinct geographic locations in the United States for response to anthracnose. A genome scan using a high-density linkage map of 3,838 single nucleotide polymorphisms (SNPs) detected two loci at 5.25 and 1.18 Mb on chromosomes 5 and 6, respectively, that explain up to 59% and 44% of the observed phenotypic variation. A bin-mapping approach using a subset of 31 highly informative RILs was employed to determine the disease response to inoculation with ten anthracnose pathotypes in the greenhouse. A genome scan showed that the 5.25 Mb region on chromosome 5 is associated with a resistance response to nine pathotypes. Five SNP markers were developed and used to fine map the locus on chromosome 5 by evaluating 1,500 segregating F_2:3progenies. Based on the genotypic and phenotypic analyses of 11 recombinants, the locus was narrowed down to a 470-kb genomic region. Following a genome-wide association study based on 574 accessions previously phenotyped and genotyped, the resistance locus was delimited to a 34-kb genomic interval with five candidate genes. All five candidate genes encode proteins associated with plant immune systems, suggesting they may act in synergy in the resistance response.

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Research Organization:: US Dept. of Agriculture (USDA), Washington, DC (United States). Agricultural Research Service

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0014171

OSTI ID:: 1627963

Journal Information:: G3, Vol. 10, Issue 4; ISSN 2160-1836

Publisher:: Genetics Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 6 works

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