skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Genetic Interaction of Aspergillus nidulans galR, xlnR and araR in Regulating D-Galactose and L-Arabinose Release and Catabolism Gene Expression

Journal Article · · PLoS ONE
 [1];  [2];  [2];  [1];  [1];  [1]
  1. Univ. of Utrecht (Netherlands). CBS-KNAW Fungal Biodiversity Centre and Fungal Molecular Physiology. Fungal Physiology
  2. Univ. of Utrecht (Netherlands). CBS-KNAW Fungal Biodiversity Centre and Fungal Molecular Physiology. Fungal Physiology; Univ. of Utrecht (Netherlands). Microbiology
+ Show Author Affiliations

In Aspergillus nidulans, the xylanolytic regulator XlnR and the arabinanolytic regulator AraR co-regulate pentose catabolism. In nature, the pentose sugars D-xylose and L-arabinose are both main building blocks of the polysaccharide arabinoxylan. In pectin and arabinogalactan, these two monosaccharides are found in combination with D-galactose. GalR, the regulator that responds to the presence of D-galactose, regulates the D-galactose catabolic pathway. In this study we investigated the possible interaction between XlnR, AraR and GalR in pentose and/or D-galactose catabolism in A. nidulans. Growth phenotypes and metabolic gene expression profiles were studied in single, double and triple disruptant A. nidulans strains of the genes encoding these paralogous transcription factors. Our results demonstrate that AraR and XlnR not only control pentose catabolic pathway genes, but also genes of the oxido-reductive D-galactose catabolic pathway. This suggests an interaction between three transcriptional regulators in D-galactose catabolism. Conversely, GalR is not involved in regulation of pentose catabolism, but controls only genes of the oxidoreductive D-galactose catabolic pathway.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1627773
Journal Information:
PLoS ONE, Vol. 10, Issue 11; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

References (30)

The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose. journal March 1996
Identification of the galactitol dehydrogenase, LadB, that is part of the oxido-reductive d-galactose catabolic pathway in Aspergillus niger journal February 2012
Hemicelluloses journal June 2010
Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction journal April 2007
Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales journal June 2011
The ‘true’ l -xylulose reductase of filamentous fungi identified in Aspergillus niger journal July 2010
Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides journal December 2001
The d-xylose reductase of Hypocrea jecorina is the major aldose reductase in pentose and d-galactose catabolism and necessary for β-galactosidase and cellulase induction by lactose journal November 2007
The metabolic role and evolution of l-arabinitol 4-dehydrogenase of Hypocrea jecorina journal May 2004
The Aspergillus niger transcriptional activator XlnR, which is involved in the degradation of the polysaccharides xylan and cellulose, also regulates d-xylose reductase gene expression journal April 2000
Regulation of formation of the intracellular β-gaiactosidase activity ofAspergillus nidulans journal January 2002
Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp. journal March 2009
The Aspergillus niger d -xylulose kinase gene is co-expressed with genes encoding arabinan degrading enzymes, and is essential for growth on d -xylose and l -arabinose: Aspergillus niger xylulose kinase journal October 2001
d-Galactose uptake is nonfunctional in the conidiospores of Aspergillus niger journal February 2012
Cloning and Expression of a Fungal l-Arabinitol 4-Dehydrogenase Gene journal August 2001
Enzyme lesions in galactose non-utilising mutants of Aspergillus nidulans journal February 1970
The alternative d-galactose degrading pathway of Aspergillus nidulans proceeds via l-sorbose journal January 2004
Identification of an l-Arabinose Reductase Gene in Aspergillus niger and Its Role in l-Arabinose Catabolism journal May 2010
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method journal December 2001
A New Black Aspergillus Species, A. vadensis, Is a Promising Host for Homologous and Heterologous Protein Production journal July 2004
Isolation and analysis of xln R, encoding a transcriptional activator co-ordinating xylanolytic expression in Aspergillus niger journal January 1998
Sybil: Methods and Software for Multiple Genome Comparison and Visualization book January 2007
Sorbitol dehydrogenase of Aspergillus niger , SdhA, is part of the oxido-reductive d -galactose pathway and essential for d -sorbitol catabolism journal January 2012
The transcriptional activators AraR and XlnR from Aspergillus niger regulate expression of pentose catabolic and pentose phosphate pathway genes journal September 2014
GalX regulates the d -galactose oxido-reductive pathway in Aspergillus niger journal October 2012
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega journal January 2011
l- xylo -3-Hexulose Reductase Is the Missing Link in the Oxidoreductive Pathway for d-Galactose Catabolism in Filamentous Fungi journal May 2012
L-Arabinose and D-Xylose Catabolism in Aspergillus niger journal August 1989
Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant journal October 1994
Unique Regulatory Mechanism for d-Galactose Utilization in Aspergillus nidulans journal August 2011

Cited By (13)

ARA1 regulates not only l -arabinose but also d -galactose catabolism in Trichoderma reesei journal December 2017
Mutations in AraR leading to constitutive of arabinolytic genes in Aspergillus niger under derepressing conditions journal April 2019
Comparison of the paralogous transcription factors AraR and XlnR in Aspergillus oryzae journal April 2018
Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste journal November 2018
Transcriptome analysis of Aspergillus niger xlnR and xkiA mutants grown on corn Stover and soybean hulls reveals a highly complex regulatory network journal November 2019
Regulators of plant biomass degradation in ascomycetous fungi journal June 2017
Myceliophthora thermophila Xyr1 is predominantly involved in xylan degradation and xylose catabolism journal September 2019
The roles of the zinc finger transcription factors XlnR, ClrA and ClrB in the breakdown of lignocellulose by Aspergillus niger journal January 2016
Identification of a Novel L-rhamnose Uptake Transporter in the Filamentous Fungus Aspergillus niger journal December 2016
Carbon Catabolite Repression in Filamentous Fungi journal December 2017
Combinatorial control of gene expression in Aspergillus niger grown on sugar beet pectin journal September 2017
Comparative transcriptome analysis reveals different strategies for degradation of steam-exploded sugarcane bagasse by Aspergillus niger and Trichoderma reesei journal June 2017
NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans journal March 2017

Similar Records

GalR, GalX and AraR co-regulate $\tiny {D}$-galactose and $\tiny{L}$-arabinose utilization in Aspergillus nidulans
Journal Article · Fri Feb 25 00:00:00 EST 2022 · Microbial Biotechnology (Online) · OSTI ID:1627773
+10 more
Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level
Journal Article · Wed Feb 02 00:00:00 EST 2022 · Fungal Genetics and Biology · OSTI ID:1627773
+9 more
High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger: Visualizing plant biomass degradation by A. niger
Journal Article · Fri Oct 20 00:00:00 EDT 2017 · Environmental Microbiology · OSTI ID:1627773
+10 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Science & Technology - Other Topics