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Title: Quantitative X-ray Tomography of the Mouse Cochlea

Journal Article · · PLoS ONE
 [1];  [2];  [3];  [4]
  1. Science and Technology Facilities Council (STFC), Oxford (United Kingdom). Diamond Light Source, Ltd.; Northwestern Univ., Evanston, IL (United States). Feinberg School of Medicine. Dept. of Otolaryngology
  2. Northwestern Univ., Evanston, IL (United States). Feinberg School of Medicine. Dept. of Otolaryngology
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
  4. Northwestern Univ., Evanston, IL (United States). Feinberg School of Medicine. Dept. of Otolaryngology; Northwestern Univ., Evanston, IL (United States). Dept. of Biomedical Engineering; Northwestern Univ., Evanston, IL (United States). The Hugh Knowles Center. Dept. of Communicaiton Sciences and Disorders
+ Show Author Affiliations

Imaging with hard X-rays allows visualizing cochlear structures while maintaining intrinsic qualities of the tissue, including structure and size. With coherent X-rays, soft tissues, including membranes, can be imaged as well as cells making use of the so-called in-line phase contrast. In the present experiments, partially coherent synchrotron radiation has been used for micro-tomography. Three-dimensional reconstructions of the mouse cochlea have been created using the EM3D software and the volume has been segmented in the Amira Software Suite. The structures that have been reconstructed include scala tympani, scala media, scala vestibuli, Reissner’s membrane, basilar membrane, tectorial membrane, organ of Corti, spiral limbus, spiral ganglion and cochlear nerve. Cross-sectional areas of the scalae were measured. The results provide a realistic and quantitative reconstruction of the cochlea.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1627504
Journal Information:
PLoS ONE, Vol. 7, Issue 4; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (10)

Fluvastatin protects cochleae from damage by high-level noise journal February 2018
Propagation-based phase-contrast x-ray tomography of cochlea using a compact synchrotron source journal March 2018
Phase contrast tomography of the mouse cochlea at microfocus x-ray sources journal August 2013
Micro-CT versus synchrotron radiation phase contrast imaging of human cochlea: MICRO-CT Vs. SYNCHROTRON RADIATION PHASE CONTRAST IMAGING journal December 2016
Effects of object-to-detector distance and beam energy on synchrotron radiation phase-contrast imaging of implanted cochleae: SR-PCI OF IMPLANTED COCHLEAE journal November 2018
Enhanced contrast in X-ray microtomographic images of the membranous labyrinth using different X-ray sources and scanning modes journal October 2018
Visualizing the 3D cytoarchitecture of the human cochlea in an intact temporal bone using synchrotron radiation phase contrast imaging journal January 2018
Visualization of the Membranous Labyrinth and Nerve Fiber Pathways in Human and Animal Inner Ears Using MicroCT Imaging journal July 2018
Three-dimensional histological specimen preparation for accurate imaging and spatial reconstruction of the middle and inner ear journal April 2013
Cochlear amplification and tuning depend on the cellular arrangement within the organ of Corti journal May 2018

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