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Title: Quantitative trait locus mapping of Populus bark features and stem diameter

Abstract

Background: Bark plays important roles in photosynthate transport and storage, along with physical and chemical protection. Bark texture varies extensively among species, from smooth to fissured to deeply furrowed, but its genetic control is unknown. This study sought to determine the main genomic regions associated with natural variation in bark features and stem diameter. Quantitative trait loci (QTL) were mapped using an interspecific pseudo-backcross pedigree ( Populus trichocarpa x P. deltoides and P. deltoides) for bark texture, bark thickness and diameter collected across three years, two sites and three biological replicates per site. Results: QTL specific to bark texture were highly reproducible in shared intervals across sites, years and replicates. Significant positive correlations and co-localization between trait QTL suggest pleiotropic regulators or closely linked genes. A list of candidate genes with related putative function, location close to QTL maxima and with the highest expression level in the phloem, xylem and cambium was identified. Conclusion: Candidate genes for bark texture included an ortholog of Arabidopsis ANAC104 (PopNAC128), which plays a role in lignified fiber cell and ray development, as well as Pinin and Fasciclin (PopFLA) genes with a role in cell adhesion, cell shape and migration. The results presented in thismore » study provide a basis for future genomic characterization of genes found within the QTL for bark texture, bark thickness and diameter in order to better understand stem and bark development in Populus and other woody perennial plants. The QTL mapping approach identified a list of prime candidate genes for further validation using functional genomics or forward genetics approaches.« less

Authors:
 [1];  [2];  [3];  [2];  [1];  [1]
  1. Michigan Technological Univ., Houghton, MI (United States). School of Forest Resources and Environmental Science
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Div.
  3. Eastern Illinois Univ., Charleston, IL (United States). Dept. of Biology
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA; National Science Foundation (NSF)
OSTI Identifier:
1626848
Grant/Contract Number:  
AC05-00OR22725; 2012–67,013-19,389; 1230803
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BMC Plant Biology
Additional Journal Information:
Journal Volume: 17; Journal Issue: 1; Journal ID: ISSN 1471-2229
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
Plant Sciences; Bark texture; Bark thickness; Diameter; Quantitative trait loci (QTL); Stem; Populus trichocarpa

Citation Formats

Bdeir, Roba, Muchero, Wellington, Yordanov, Yordan, Tuskan, Gerald A., Busov, Victor, and Gailing, Oliver. Quantitative trait locus mapping of Populus bark features and stem diameter. United States: N. p., 2017. Web. doi:10.1186/s12870-017-1166-4.
Bdeir, Roba, Muchero, Wellington, Yordanov, Yordan, Tuskan, Gerald A., Busov, Victor, & Gailing, Oliver. Quantitative trait locus mapping of Populus bark features and stem diameter. United States. https://doi.org/10.1186/s12870-017-1166-4
Bdeir, Roba, Muchero, Wellington, Yordanov, Yordan, Tuskan, Gerald A., Busov, Victor, and Gailing, Oliver. Tue . "Quantitative trait locus mapping of Populus bark features and stem diameter". United States. https://doi.org/10.1186/s12870-017-1166-4. https://www.osti.gov/servlets/purl/1626848.
@article{osti_1626848,
title = {Quantitative trait locus mapping of Populus bark features and stem diameter},
author = {Bdeir, Roba and Muchero, Wellington and Yordanov, Yordan and Tuskan, Gerald A. and Busov, Victor and Gailing, Oliver},
abstractNote = {Background: Bark plays important roles in photosynthate transport and storage, along with physical and chemical protection. Bark texture varies extensively among species, from smooth to fissured to deeply furrowed, but its genetic control is unknown. This study sought to determine the main genomic regions associated with natural variation in bark features and stem diameter. Quantitative trait loci (QTL) were mapped using an interspecific pseudo-backcross pedigree (Populus trichocarpa x P. deltoides and P. deltoides) for bark texture, bark thickness and diameter collected across three years, two sites and three biological replicates per site. Results: QTL specific to bark texture were highly reproducible in shared intervals across sites, years and replicates. Significant positive correlations and co-localization between trait QTL suggest pleiotropic regulators or closely linked genes. A list of candidate genes with related putative function, location close to QTL maxima and with the highest expression level in the phloem, xylem and cambium was identified. Conclusion: Candidate genes for bark texture included an ortholog of Arabidopsis ANAC104 (PopNAC128), which plays a role in lignified fiber cell and ray development, as well as Pinin and Fasciclin (PopFLA) genes with a role in cell adhesion, cell shape and migration. The results presented in this study provide a basis for future genomic characterization of genes found within the QTL for bark texture, bark thickness and diameter in order to better understand stem and bark development in Populus and other woody perennial plants. The QTL mapping approach identified a list of prime candidate genes for further validation using functional genomics or forward genetics approaches.},
doi = {10.1186/s12870-017-1166-4},
url = {https://www.osti.gov/biblio/1626848}, journal = {BMC Plant Biology},
issn = {1471-2229},
number = 1,
volume = 17,
place = {United States},
year = {2017},
month = {11}
}

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Works referenced in this record:

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    Works referencing / citing this record:

    Genome-wide association studies of bark texture in Populus trichocarpa
    journal, February 2019


    Bark Features for Identifying Resonance Spruce Standing Timber
    journal, September 2019