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Title: chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.

Abstract

Chlorophylls (Chls) play pivotal roles in energy absorption and transduction and also in charge separation in reaction centers in all photosynthetic organisms. In Chl biosynthesis steps, only a step for the enzymatic reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) is mediated by both nuclear- and chloroplast-encoded genes in land plants. Many plants encode the genes for light-dependent Pchlide reductase (LPOR) and light-independent Pchlide reductase (DPOR) in the nucleus and chloroplast genome, respectively. During the diversification of land plants, the reduction step of Pchlide to Chlide has become solely dependent on LPOR, and the genes for DPOR have been lost from chloroplast genome. It remains unclear why DPOR persists in some land plants, how they were eliminated from chloroplast genomes during the diversification of land plants, and under what environmental conditions DPOR was required. We demonstrate that DPOR is functional in liverwort (Marchantia polymorpha L.) and plays an important role in Chl biosynthesis. Having established a plastid transformation system in liverwort, we disrupted chlB, which encodes a subunit of DPOR in the M. polymorpha chloroplast genome. Morphological and Chl content analysis of a chlB mutant grown under different photoperiods revealed that DPOR is particularly required for Chl biosynthesis under short-day conditions.more » Our findings suggest that an environmental condition in the form of photoperiod is an important factor that determines the loss or retention of chloroplast-encoded genes mediating Pchlide reduction to Chlide.« less

Authors:
 [1];  [2];  [1];  [1];  [1]
  1. Kyoto Univ. (Japan). Graduate School of Science. Dept. of Botany
  2. Hokkaido Univ., Sapporo (Japan). Inst. of Low Temperature Science
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1625333
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Genome Biology and Evolution
Additional Journal Information:
Journal Volume: 6; Journal Issue: 3; Journal ID: ISSN 1759-6653
Publisher:
Society for Molecular Biology and Evolution
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; Evolutionary Biology; Genetics & Heredity; plastid transformation; chloroplast genome evolution; bryophyte; chlorophyll metabolism

Citation Formats

Ueda, Minoru, Tanaka, Ayumi, Sugimoto, Kazuhiko, Shikanai, Toshiharu, and Nishimura, Yoshiki. chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.. United States: N. p., 2014. Web. doi:10.1093/gbe/evu045.
Ueda, Minoru, Tanaka, Ayumi, Sugimoto, Kazuhiko, Shikanai, Toshiharu, & Nishimura, Yoshiki. chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.. United States. https://doi.org/10.1093/gbe/evu045
Ueda, Minoru, Tanaka, Ayumi, Sugimoto, Kazuhiko, Shikanai, Toshiharu, and Nishimura, Yoshiki. 2014. "chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.". United States. https://doi.org/10.1093/gbe/evu045. https://www.osti.gov/servlets/purl/1625333.
@article{osti_1625333,
title = {chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.},
author = {Ueda, Minoru and Tanaka, Ayumi and Sugimoto, Kazuhiko and Shikanai, Toshiharu and Nishimura, Yoshiki},
abstractNote = {Chlorophylls (Chls) play pivotal roles in energy absorption and transduction and also in charge separation in reaction centers in all photosynthetic organisms. In Chl biosynthesis steps, only a step for the enzymatic reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) is mediated by both nuclear- and chloroplast-encoded genes in land plants. Many plants encode the genes for light-dependent Pchlide reductase (LPOR) and light-independent Pchlide reductase (DPOR) in the nucleus and chloroplast genome, respectively. During the diversification of land plants, the reduction step of Pchlide to Chlide has become solely dependent on LPOR, and the genes for DPOR have been lost from chloroplast genome. It remains unclear why DPOR persists in some land plants, how they were eliminated from chloroplast genomes during the diversification of land plants, and under what environmental conditions DPOR was required. We demonstrate that DPOR is functional in liverwort (Marchantia polymorpha L.) and plays an important role in Chl biosynthesis. Having established a plastid transformation system in liverwort, we disrupted chlB, which encodes a subunit of DPOR in the M. polymorpha chloroplast genome. Morphological and Chl content analysis of a chlB mutant grown under different photoperiods revealed that DPOR is particularly required for Chl biosynthesis under short-day conditions. Our findings suggest that an environmental condition in the form of photoperiod is an important factor that determines the loss or retention of chloroplast-encoded genes mediating Pchlide reduction to Chlide.},
doi = {10.1093/gbe/evu045},
url = {https://www.osti.gov/biblio/1625333}, journal = {Genome Biology and Evolution},
issn = {1759-6653},
number = 3,
volume = 6,
place = {United States},
year = {2014},
month = {2}
}

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