Demonstration of femtosecond X-ray pump X-ray probe diffraction on protein crystals

Opara, Nadia L.; Mohacsi, Istvan; Makita, Mikako; Castano-Diez, Daniel; Diaz, Ana; Juranić, Pavle; Marsh, May; Meents, Alke; Milne, Christopher J.; Mozzanica, Aldo; Padeste, Celestino; Panneels, Valérie; Sikorski, Marcin; Song, Sanghoon; Stahlberg, Henning; Vartiainen, Ismo; Vera, Laura; Wang, Meitian; Willmott, Philip R.; David, Christian

doi:10.1063/1.5050618

Title: Demonstration of femtosecond X-ray pump X-ray probe diffraction on protein crystals

Journal Article · Mon Oct 01 00:00:00 EDT 2018 · Structural Dynamics

DOI:https://doi.org/10.1063/1.5050618· OSTI ID:1625000

Opara, Nadia L. ^[1]; Mohacsi, Istvan ^[2]; Makita, Mikako ^[3]; Castano-Diez, Daniel ^[4]; Diaz, Ana ^[5]; Juranić, Pavle ^[5]; Marsh, May ^[5]; Meents, Alke ^[6]; Milne, Christopher J. ^[5]; Mozzanica, Aldo ^[5]; Padeste, Celestino ^[5]; Panneels, Valérie ^[5]; Sikorski, Marcin ^[7]; Song, Sanghoon ^[7]; Stahlberg, Henning ^[8]; Vartiainen, Ismo ^[5]; Vera, Laura ^[5]; Wang, Meitian ^[5]; Willmott, Philip R. ^[5]; David, Christian ^[5]

Paul Scherrer Inst. (Switzerland); Univ. of Basel (Switzerland); Swiss Nanoscience Inst., Basel, (Switzerland)
Paul Scherrer Inst. (Switzerland); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
Paul Scherrer Inst. (Switzerland); European XFEL GmbH, Schenefeld (Germany)
Univ. of Basel (Switzerland)
Paul Scherrer Inst. (Switzerland)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Univ. of Basel (Switzerland); Swiss Nanoscience Inst., Basel, (Switzerland)

The development of X-ray free-electron lasers (XFELs) has opened the possibility to investigate the ultrafast dynamics of biomacromolecules using X-ray diffraction. Whereas an increasing number of structures solved by means of serial femtosecond crystallography at XFELs is available, the effect of radiation damage on protein crystals during ultrafast exposures has remained an open question. We used a splitand-delay line based on diffractive X-ray optics at the Linac Coherent Light Source XFEL to investigate the time dependence of X-ray radiation damage to lysozyme crystals. For these tests, crystals were delivered to the X-ray beam using a fixed-target approach. The presented experiments provide probe signals at eight different delay times between 19 and 213 femtoseconds after a single pump event, thereby covering the time-scales relevant for femtosecond serial crystallography. Even though significant impact on the crystals was observed at long time scales after exposure with a single X-ray pulse, the collected diffraction data did not show significant signal reduction that could be assigned to beam damage on the crystals in the sampled time window and resolution range. This observation is in agreement with estimations of the applied radiation dose, which in our experiment was clearly below the values expected to cause damage on the femtosecond time scale. The experiments presented here demonstrate the feasibility of time-resolved pump-multiprobe X-ray diffraction experiments on protein crystals.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Linac Coherent Light Source (LCLS); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); European Community’s Seventh Framework Programme; Swiss Nanoscience Institute

Grant/Contract Number:: AC02-76SF00515; AC52-07NA27344; 290605

OSTI ID:: 1625000

Alternate ID(s):: OSTI ID: 1475093

Journal Information:: Structural Dynamics, Vol. 5, Issue 5; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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Perspective: Towards single shot time-resolved microscopy using short wavelength table-top light sources Helk, T.; Zürch, M.; Spielmann, C. GSI Helmholtzzentrum fuer Schwerionenforschung, GSI, Darmstadt https://doi.org/10.15120/gsi-2019-00570	text	January 2019
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Perspective: Towards single shot time-resolved microscopy using short wavelength table-top light sources Helk, T.; Zürch, M.; Spielmann, C. Structural Dynamics, Vol. 6, Issue 1 https://doi.org/10.1063/1.5082686	journal	January 2019
X-rays put molecules into a spin Costello, John T.; Manson, Steven T. Proceedings of the National Academy of Sciences, Vol. 116, Issue 11 https://doi.org/10.1073/pnas.1900971116	journal	February 2019

Figures / Tables (7)

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FIG. 5(p. 11)

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Related Subjects

43 PARTICLE ACCELERATORS
59 BASIC BIOLOGICAL SCIENCES
Chemistry
Physics
Ultrafast time-resolved crystallography
Free electron lasers
Physical radiation effects
Delay lines
Protein crystallography
X-ray optics
Pump probe experiments
Radiation damage
X-ray diffraction
Ultrafast optics