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Title: Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation

Abstract

This study used 13C tracers and dynamic labeling to reveal metabolic features (nutrients requirements, pathway delineation and metabolite turnover rates) of Clostridium carboxidivorans P7, a model strain for industrial syngas fermentation, and its implication with bioreactor mass transfer. P7 shows poor activity for synthesizing amino acids (e.g., phenylalanine) and thus, needs rich medium for cell growth. The strain has multiple carbon fxation routes (Wood-Ljungdahl pathway, pyruvate:ferredoxin oxidoreductase reaction and anaplerotic pathways) and Re-citrate synthase (Ccar_06155) was a key enzyme in its tricarboxylic acid cycle (TCA) pathway. High fuxes were observed in P7’s Wood-Ljungdahl pathway, right branch of TCA cycle, pyruvate synthesis, and sugar phosphate pathways, but the cells anabolic pathways were strikingly slow. In bioreactor culture, when syngas fowrate increased from 1 to 10mL/min, P7 strain produced same amount of total extracellular products (acids and alcohols) but high fowrate favored alcohol accumulation. This observation was due to the mass transfer limitation infuencing energy metabolism (CO/H2 oxidation for cofactor generations) more prominently than carbon fxation. When syngas fowrate increased from 10 of 20mL/min, the alcohol productivity was not improved and the labeling rate (~0.03h -1) of key metabolite acetyl-CoA reached to P7 strain’s metabolism limitation regime.

Authors:
 [1];  [2];  [3];  [3];  [4]
  1. Washington Univ., St. Louis, MO (United States). Dept. of Mechanical Engineering and Materials Science
  2. Iowa State Univ., Ames, IA (United States). Agricultural and Biosystems Engineering Dept.
  3. Washington Univ., St. Louis, MO (United States). Dept. of Energy, Environmental and Chemical Engineering
  4. Iowa State Univ., Ames, IA (United States). Dept. of Food Science and Human Nutrition
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1624332
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics

Citation Formats

Wan, Ni, Sathish, Ashik, You, Le, Tang, Yinjie J., and Wen, Zhiyou. Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation. United States: N. p., 2017. Web. doi:10.1038/s41598-017-10312-2.
Wan, Ni, Sathish, Ashik, You, Le, Tang, Yinjie J., & Wen, Zhiyou. Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation. United States. https://doi.org/10.1038/s41598-017-10312-2
Wan, Ni, Sathish, Ashik, You, Le, Tang, Yinjie J., and Wen, Zhiyou. Wed . "Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation". United States. https://doi.org/10.1038/s41598-017-10312-2. https://www.osti.gov/servlets/purl/1624332.
@article{osti_1624332,
title = {Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation},
author = {Wan, Ni and Sathish, Ashik and You, Le and Tang, Yinjie J. and Wen, Zhiyou},
abstractNote = {This study used 13C tracers and dynamic labeling to reveal metabolic features (nutrients requirements, pathway delineation and metabolite turnover rates) of Clostridium carboxidivorans P7, a model strain for industrial syngas fermentation, and its implication with bioreactor mass transfer. P7 shows poor activity for synthesizing amino acids (e.g., phenylalanine) and thus, needs rich medium for cell growth. The strain has multiple carbon fxation routes (Wood-Ljungdahl pathway, pyruvate:ferredoxin oxidoreductase reaction and anaplerotic pathways) and Re-citrate synthase (Ccar_06155) was a key enzyme in its tricarboxylic acid cycle (TCA) pathway. High fuxes were observed in P7’s Wood-Ljungdahl pathway, right branch of TCA cycle, pyruvate synthesis, and sugar phosphate pathways, but the cells anabolic pathways were strikingly slow. In bioreactor culture, when syngas fowrate increased from 1 to 10mL/min, P7 strain produced same amount of total extracellular products (acids and alcohols) but high fowrate favored alcohol accumulation. This observation was due to the mass transfer limitation infuencing energy metabolism (CO/H2 oxidation for cofactor generations) more prominently than carbon fxation. When syngas fowrate increased from 10 of 20mL/min, the alcohol productivity was not improved and the labeling rate (~0.03h-1) of key metabolite acetyl-CoA reached to P7 strain’s metabolism limitation regime.},
doi = {10.1038/s41598-017-10312-2},
url = {https://www.osti.gov/biblio/1624332}, journal = {Scientific Reports},
issn = {2045-2322},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {8}
}

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    Works referencing / citing this record:

    Domestication of the novel alcohologenic acetogen Clostridium sp. AWRP: from isolation to characterization for syngas fermentation
    journal, September 2019


    Translational Metabolomics: Current Challenges and Future Opportunities
    journal, June 2019


    Incorporating hydrodynamics into spatiotemporal metabolic models of bubble column gas fermentation: LI et al.
    journal, October 2018


    Two stirred-tank bioreactors in series enable continuous production of alcohols from carbon monoxide with Clostridium carboxidivorans
    journal, July 2018


    Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans
    journal, January 2020