Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals
Abstract
Naturally, many aerobic organisms degrade lignin-derived aromatics through conserved intermediates including protocatechuate and catechol. Employing this microbial approach offers a potential solution for valorizing lignin into valuable chemicals for a potential lignocellulosic biorefinery and enabling bioeconomy. In this study, two hybrid biochemical routes combining lignin chemical depolymerization, plant metabolic engineering, and synthetic pathway reconstruction were demonstrated for valorizing lignin into value-added products. In the biochemical route 1, alkali lignin was chemically depolymerized into vanillin and syringate as major products, which were further bio-converted into cis, cis-muconic acid (ccMA) and pyrogallol, respectively, using engineered Escherichia coli strains. In the second biochemical route, the shikimate pathway of Tobacco plant was engineered to accumulate protocatechuate (PCA) as a soluble intermediate compound. The PCA extracted from the engineered Tobacco was further converted into ccMA using the engineered E. coli strain. This study reports a direct process for converting lignin into ccMA and pyrogallol as value-added chemicals, and more importantly demonstrates benign methods for valorization of polymeric lignin that is inherently heterogeneous and recalcitrant. Our approach also validates the promising combination of plant engineering with microbial chassis development for the production of value added and speciality chemicals.
- Authors:
-
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biomass Science & Conversion Technologies
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biomass Science & Conversion Technologies
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biomass Science & Conversion Technologies; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Bioproducts and Biosystems Engineering
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1624321
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Science & Technology - Other Topics
Citation Formats
Wu, Weihua, Dutta, Tanmoy, Varman, Arul M., Eudes, Aymerick, Manalansan, Bianca, Loqué, Dominique, and Singh, Seema. Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals. United States: N. p., 2017.
Web. doi:10.1038/s41598-017-07895-1.
Wu, Weihua, Dutta, Tanmoy, Varman, Arul M., Eudes, Aymerick, Manalansan, Bianca, Loqué, Dominique, & Singh, Seema. Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals. United States. https://doi.org/10.1038/s41598-017-07895-1
Wu, Weihua, Dutta, Tanmoy, Varman, Arul M., Eudes, Aymerick, Manalansan, Bianca, Loqué, Dominique, and Singh, Seema. 2017.
"Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals". United States. https://doi.org/10.1038/s41598-017-07895-1. https://www.osti.gov/servlets/purl/1624321.
@article{osti_1624321,
title = {Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals},
author = {Wu, Weihua and Dutta, Tanmoy and Varman, Arul M. and Eudes, Aymerick and Manalansan, Bianca and Loqué, Dominique and Singh, Seema},
abstractNote = {Naturally, many aerobic organisms degrade lignin-derived aromatics through conserved intermediates including protocatechuate and catechol. Employing this microbial approach offers a potential solution for valorizing lignin into valuable chemicals for a potential lignocellulosic biorefinery and enabling bioeconomy. In this study, two hybrid biochemical routes combining lignin chemical depolymerization, plant metabolic engineering, and synthetic pathway reconstruction were demonstrated for valorizing lignin into value-added products. In the biochemical route 1, alkali lignin was chemically depolymerized into vanillin and syringate as major products, which were further bio-converted into cis, cis-muconic acid (ccMA) and pyrogallol, respectively, using engineered Escherichia coli strains. In the second biochemical route, the shikimate pathway of Tobacco plant was engineered to accumulate protocatechuate (PCA) as a soluble intermediate compound. The PCA extracted from the engineered Tobacco was further converted into ccMA using the engineered E. coli strain. This study reports a direct process for converting lignin into ccMA and pyrogallol as value-added chemicals, and more importantly demonstrates benign methods for valorization of polymeric lignin that is inherently heterogeneous and recalcitrant. Our approach also validates the promising combination of plant engineering with microbial chassis development for the production of value added and speciality chemicals.},
doi = {10.1038/s41598-017-07895-1},
url = {https://www.osti.gov/biblio/1624321},
journal = {Scientific Reports},
issn = {2045-2322},
number = 1,
volume = 7,
place = {United States},
year = {Mon Aug 21 00:00:00 EDT 2017},
month = {Mon Aug 21 00:00:00 EDT 2017}
}
Web of Science
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