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Title: Real-time intermembrane force measurements and imaging of lipid domain morphology during hemifusion

Abstract

Membrane fusion is the core process in membrane trafficking and is essential for cellular transport of proteins and other biomacromolecules. During protein-mediated membrane fusion, membrane proteins are often excluded from the membrane–membrane contact, indicating that local structural transformations in lipid domains play a major role. However, the rearrangements of lipid domains during fusion have not been thoroughly examined. Here using a newly developed Fluorescence Surface Forces Apparatus (FL-SFA), migration of liquid-disordered clusters and depletion of liquid-ordered domains at the membrane–membrane contact are imaged in real time during hemifusion of model lipid membranes, together with simultaneous force–distance and lipid membrane thickness measurements. The load and contact time-dependent hemifusion results show that the domain rearrangements decrease the energy barrier to fusion, illustrating the significance of dynamic domain transformations in membrane fusion processes. Importantly, the FL-SFA can unambiguously correlate interaction forces and in situ imaging in many dynamic interfacial systems.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3]
  1. Univ. of California, Santa Barbara, CA (United States). Dept. of Chemical Engineering
  2. Univ. of Montreal, QC (Canada). Canada Research Chair in Bio-inspired Materials and Interfaces. Faculty of Pharmacy
  3. Univ. of California, Santa Barbara, CA (United States). Dept. of Chemical Engineering. Dept. of Materials
Publication Date:
Research Org.:
Univ. of California, Santa Barbara, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1623977
Grant/Contract Number:  
FG02-87ER45331
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE; Membrane lipids; Membrane fusion; Membrane biophysics

Citation Formats

Lee, Dong Woog, Kristiansen, Kai, Donaldson, Stephen H., Cadirov, Nicholas, Banquy, Xavier, and Israelachvili, Jacob N. Real-time intermembrane force measurements and imaging of lipid domain morphology during hemifusion. United States: N. p., 2015. Web. doi:10.1038/ncomms8238.
Lee, Dong Woog, Kristiansen, Kai, Donaldson, Stephen H., Cadirov, Nicholas, Banquy, Xavier, & Israelachvili, Jacob N. Real-time intermembrane force measurements and imaging of lipid domain morphology during hemifusion. United States. https://doi.org/10.1038/ncomms8238
Lee, Dong Woog, Kristiansen, Kai, Donaldson, Stephen H., Cadirov, Nicholas, Banquy, Xavier, and Israelachvili, Jacob N. Tue . "Real-time intermembrane force measurements and imaging of lipid domain morphology during hemifusion". United States. https://doi.org/10.1038/ncomms8238. https://www.osti.gov/servlets/purl/1623977.
@article{osti_1623977,
title = {Real-time intermembrane force measurements and imaging of lipid domain morphology during hemifusion},
author = {Lee, Dong Woog and Kristiansen, Kai and Donaldson, Stephen H. and Cadirov, Nicholas and Banquy, Xavier and Israelachvili, Jacob N.},
abstractNote = {Membrane fusion is the core process in membrane trafficking and is essential for cellular transport of proteins and other biomacromolecules. During protein-mediated membrane fusion, membrane proteins are often excluded from the membrane–membrane contact, indicating that local structural transformations in lipid domains play a major role. However, the rearrangements of lipid domains during fusion have not been thoroughly examined. Here using a newly developed Fluorescence Surface Forces Apparatus (FL-SFA), migration of liquid-disordered clusters and depletion of liquid-ordered domains at the membrane–membrane contact are imaged in real time during hemifusion of model lipid membranes, together with simultaneous force–distance and lipid membrane thickness measurements. The load and contact time-dependent hemifusion results show that the domain rearrangements decrease the energy barrier to fusion, illustrating the significance of dynamic domain transformations in membrane fusion processes. Importantly, the FL-SFA can unambiguously correlate interaction forces and in situ imaging in many dynamic interfacial systems.},
doi = {10.1038/ncomms8238},
url = {https://www.osti.gov/biblio/1623977}, journal = {Nature Communications},
issn = {2041-1723},
number = 1,
volume = 6,
place = {United States},
year = {2015},
month = {5}
}

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Cited by: 14 works
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