DNA-SIP based genome-centric metagenomics identifies key long-chain fatty acid-degrading populations in anaerobic digesters with different feeding frequencies

Ziels, Ryan M.; Sousa, Diana Z.; Stensel, H. David; Beck, David A. C.

doi:10.1038/ismej.2017.143

Title: DNA-SIP based genome-centric metagenomics identifies key long-chain fatty acid-degrading populations in anaerobic digesters with different feeding frequencies

Journal Article · Tue Sep 12 00:00:00 EDT 2017 · The ISME Journal

DOI:https://doi.org/10.1038/ismej.2017.143· OSTI ID:1623778

Ziels, Ryan M. ^[1]; Sousa, Diana Z. ^[2]; Stensel, H. David ^[3]; Beck, David A. C. ^[4]

Univ. of British Columbia, Vancouver, BC (Canada). Dept. of Civil Engineering; Univ. of Washington, Seattle, WA (United States). Dept. of Civil and Environmental Engineering
Wageningen Univ. (Netherlands). Lab. of Microbiology
Univ. of Washington, Seattle, WA (United States). Dept. of Civil and Environmental Engineering
Univ. of Washington, Seattle, WA (United States). eScience Inst.; Univ. of Washington, Seattle, WA (United States). Dept. of Chemical Engineering

Fats, oils and greases (FOG) are energy-dense wastes that can be added to anaerobic digesters to substantially increase biomethane recovery via their conversion through long-chain fatty acids (LCFAs). However, a better understanding of the ecophysiology of syntrophic LCFA-degrading microbial communities in anaerobic digesters is needed to develop operating strategies that mitigate inhibitory LCFA accumulation from FOG. In this research, DNA stable isotope probing (SIP) was coupled with metagenomic sequencing for a genome-centric comparison of oleate (C18:1)-degrading populations in two anaerobic codigesters operated with either a pulse feeding or continuous-feeding strategy. The pulse-fed codigester microcosms converted oleate into methane at over 20% higher rates than the continuous-fed codigester microcosms. Differential coverage binning was demonstrated for the first time to recover population genome bins (GBs) from DNA-SIP metagenomes. About 70% of the 13C-enriched GBs were taxonomically assigned to the Syntrophomonas genus, thus substantiating the importance of Syntrophomonas species to LCFA degradation in anaerobic digesters. Phylogenetic comparisons of 13C-enriched GBs showed that phylogenetically distinct Syntrophomonas GBs were unique to each codigester. Overall, these results suggest that syntrophic populations in anaerobic digesters can have different adaptive capacities, and that selection for divergent populations may be achieved by adjusting reactor operating conditions to maximize biomethane recovery.

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Research Organization:: USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: AC02-05CH11231; US EPA STAR [RD835567]; US DOE JGI [CSP 2791]; ERC [323009]

OSTI ID:: 1623778

Journal Information:: The ISME Journal, Vol. 12, Issue 1; ISSN 1751-7362

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

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