Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae

Park, Chang-Jin; Wei, Tong; Sharma, Rita; Ronald, Pamela C.

doi:10.1186/s12284-017-0166-1

Title: Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae

Journal Article · Fri Jun 02 00:00:00 EDT 2017 · Rice

DOI:https://doi.org/10.1186/s12284-017-0166-1· OSTI ID:1619456

Park, Chang-Jin; Wei, Tong; Sharma, Rita; Ronald, Pamela C.

The rice immune receptor XA21 confers resistance to the bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo). To elucidate the mechanism of XA21-mediated immunity, we previously performed a yeast two-hybrid screening for XA21 interactors and identified XA21 binding protein 21 (XB21). Here, we report that XB21 is an auxilin-like protein predicted to function in clathrin-mediated endocytosis. We demonstrate an XA21/XB21 in vivo interaction using co-immunoprecipitation in rice. Overexpression of XB21 in rice variety Kitaake and a Kitaake transgenic line expressing XA21 confers a necrotic lesion phenotype and enhances resistance to Xoo. RNA sequencing reveals that XB21 overexpression results in the differential expression of 8735 genes (4939 genes up- and 3846 genes down-regulated) (≥2-folds, FDR ≤0.01). The up-regulated genes include those predicted to be involved in ‘cell death’ and ‘vesicle-mediated transport’. These results indicate that XB21 plays a role in the plant immune response and in regulation of cell death. The up-regulation of genes controlling ‘vesicle-mediated transport’ in XB21 overexpression lines is consistent with a functional role for XB21 as an auxilin.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231; IOS-0817738; GM55962

OSTI ID:: 1619456

Alternate ID(s):: OSTI ID: 1379882

Journal Information:: Rice, Journal Name: Rice Vol. 10 Journal Issue: 1; ISSN 1939-8425

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Japan

Language:: English

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Cited by: 13 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Auxilin
Auxilin-Like Protein
Disease Resistance
Heat Shock Cognate Protein
Heat Shock Protein
XA21 Immune Receptor
XA21 Binding Protein 21
Xanthomonas oryzae pv. oryzae

Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae [Supplemental Data] Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593 The current record is supplemented by this collection	collection	June 2017
Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae [Supplemental Data] Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593.v1 The current record is supplemented by this dataset	dataset	June 2017
Additional file 1: Figure S1. of Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593_d1 The current record is supplemented by this dataset	dataset	June 2017
Additional file 1: Figure S1. of Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593_d1.v1 The current record is supplemented by this dataset	dataset	June 2017
Additional file 2: Table S1. of Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593_d2 The current record is supplemented by this dataset	dataset	June 2017
Additional file 2: Table S1. of Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593_d2.v1 The current record is supplemented by this dataset	dataset	June 2017
Additional file 3: Table S2. of Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593_d3 The current record is supplemented by this dataset	dataset	June 2017
Additional file 3: Table S2. of Overexpression of Rice Auxilin-Like Protein, XB21, Induces Necrotic Lesions, up-Regulates Endocytosis-Related Genes, and Confers Enhanced Resistance to Xanthomonas oryzae pv. oryzae Park, Chang-Jin; Wei, Tong; Sharma, Rita https://doi.org/10.6084/m9.figshare.c.3794593_d3.v1 The current record is supplemented by this dataset	dataset	June 2017