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Title: Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability

Abstract

BackgroundSeasonal variation in microalgae production is a significant challenge to developing cost-competitive algae biofuels. Summer production can be three to five times greater than winter production, which may result in winter biomass shortages and summer surpluses at algae biorefineries. While the high water content (80%, wet basis) of harvested microalgae biomass makes drying an expensive approach to preservation, it is not an issue for ensiling. Ensiling relies on lactic acid fermentation to create anaerobic acidic conditions, which limits further microbial degradation. This study investigates the feasibility of preserving microalgae biomass through wet anaerobic storage ensiling over 30 and 180 days of storage, and it presents a techno-economic analysis that considers potential cost implications.ResultsHarvested Scenedesmus acutus biomass untreated (anaerobic) or supplemented with 0.5% sulfuric acid underwent robust lactic acid fermentation (lactic acid content of 6–9%, dry basis) lowering the pH to 4.2. Dry matter losses after 30 days ranged from 10.8 to 15.5% depending on the strain and treatment without additional loss over the next 150 days. Long-term storage of microalgae biomass resulted in lactic acid concentrations that remained high (6%, dry basis) with a low pH (4.2–4.6). Detailed biochemical composition revealed that protein and lipid content remained unaffected by storagemore » while carbohydrate content was reduced, with greater dry matter loss associated with greater reduction in carbohydrate content, primarily affecting glucan content. Techno-economic analysis comparing wet storage to drying and dry storage demonstrated the cost savings of this approach. The most realistic dry storage scenario assumes a contact drum dryer and aboveground carbon steel storage vessels, which translates to a minimum fuel selling price (MFSP) of 3.72 dollars/gallon gasoline equivalent (GGE), whereas the most realistic wet storage scenario, which includes belowground, covered wet storage pits translates to an MFSP of 3.40 dollars/GGE.ConclusionsMicroalgae biomass can be effectively preserved through wet anaerobic storage, limiting dry matter loss to below 10% over 6 months with minimal degradation of carbohydrates and preservation of lipids and proteins. Techno-economic analysis indicates that wet storage can reduce overall biomass and fuel costs compared to drying and dry storage.« less

Authors:
ORCiD logo; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1618755
Alternate Identifier(s):
OSTI ID: 1512669
Report Number(s):
NREL/JA-5100-71994
Journal ID: ISSN 1754-6834; 80; PII: 1420
Grant/Contract Number:  
AC07-05ID14517; AC36-08GO28308
Resource Type:
Journal Article: Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; microalgae; stabilization; anaerobic storage; ensiling; techno-economic analysis

Citation Formats

Wendt, Lynn M., Kinchin, Christopher, Wahlen, Bradley D., Davis, Ryan, Dempster, Thomas A., and Gerken, Henri. Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability. Netherlands: N. p., 2019. Web. doi:10.1186/s13068-019-1420-0.
Wendt, Lynn M., Kinchin, Christopher, Wahlen, Bradley D., Davis, Ryan, Dempster, Thomas A., & Gerken, Henri. Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability. Netherlands. https://doi.org/10.1186/s13068-019-1420-0
Wendt, Lynn M., Kinchin, Christopher, Wahlen, Bradley D., Davis, Ryan, Dempster, Thomas A., and Gerken, Henri. 2019. "Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability". Netherlands. https://doi.org/10.1186/s13068-019-1420-0.
@article{osti_1618755,
title = {Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability},
author = {Wendt, Lynn M. and Kinchin, Christopher and Wahlen, Bradley D. and Davis, Ryan and Dempster, Thomas A. and Gerken, Henri},
abstractNote = {BackgroundSeasonal variation in microalgae production is a significant challenge to developing cost-competitive algae biofuels. Summer production can be three to five times greater than winter production, which may result in winter biomass shortages and summer surpluses at algae biorefineries. While the high water content (80%, wet basis) of harvested microalgae biomass makes drying an expensive approach to preservation, it is not an issue for ensiling. Ensiling relies on lactic acid fermentation to create anaerobic acidic conditions, which limits further microbial degradation. This study investigates the feasibility of preserving microalgae biomass through wet anaerobic storage ensiling over 30 and 180 days of storage, and it presents a techno-economic analysis that considers potential cost implications.ResultsHarvested Scenedesmus acutus biomass untreated (anaerobic) or supplemented with 0.5% sulfuric acid underwent robust lactic acid fermentation (lactic acid content of 6–9%, dry basis) lowering the pH to 4.2. Dry matter losses after 30 days ranged from 10.8 to 15.5% depending on the strain and treatment without additional loss over the next 150 days. Long-term storage of microalgae biomass resulted in lactic acid concentrations that remained high (6%, dry basis) with a low pH (4.2–4.6). Detailed biochemical composition revealed that protein and lipid content remained unaffected by storage while carbohydrate content was reduced, with greater dry matter loss associated with greater reduction in carbohydrate content, primarily affecting glucan content. Techno-economic analysis comparing wet storage to drying and dry storage demonstrated the cost savings of this approach. The most realistic dry storage scenario assumes a contact drum dryer and aboveground carbon steel storage vessels, which translates to a minimum fuel selling price (MFSP) of 3.72 dollars/gallon gasoline equivalent (GGE), whereas the most realistic wet storage scenario, which includes belowground, covered wet storage pits translates to an MFSP of 3.40 dollars/GGE.ConclusionsMicroalgae biomass can be effectively preserved through wet anaerobic storage, limiting dry matter loss to below 10% over 6 months with minimal degradation of carbohydrates and preservation of lipids and proteins. Techno-economic analysis indicates that wet storage can reduce overall biomass and fuel costs compared to drying and dry storage.},
doi = {10.1186/s13068-019-1420-0},
url = {https://www.osti.gov/biblio/1618755}, journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 12,
place = {Netherlands},
year = {2019},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1186/s13068-019-1420-0

Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

Figures / Tables:

Table 1 Table 1: Storage performance of Scenedesmus acutus biomass after 30 or 180 days of wet anaerobic storage

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.