Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight
Abstract
Background: Camelina (Camelina sativa L.) is a promising oilseed crop that may provide sustainable feedstock for biofuel production. One of the major drawbacks of Camelina is its smaller seeds compared to other major oil crops such as canola, which limit oil yield and may also pose challenges in successful seedling establishment, especially in dryland cultivation. Previous studies indicate that seed development may be under metabolic control. In oilseeds, starch only accumulates temporarily during seed development but is almost absent in mature seeds. In this study, we explored the efect of altering seed carbohydrate metabolism on Camelina seed size through down-regulating ADPglucose pyrophosphorylase (AGPase), a major enzyme in starch biosynthesis. Results: An RNAi construct comprising sequences of the Camelina small subunit of an AGPase (CsAPS) was expressed in Camelina cultivar Suneson under a seed-specifc promoter. The RNAi suppression reduced AGPase activities which concurred with moderately decreased starch accumulation during seed development. Transcripts of genes examined that are involved in storage products were not afected, but contents of sugars and water were increased in developing seeds. The transgenic seeds were larger than wild-type plants due to increased cell sizes in seed coat and embryos, and mature seeds contained similar oil but moremore »
- Authors:
- Publication Date:
- Research Org.:
- Montana State Univ., Bozeman, MT (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1618745
- Alternate Identifier(s):
- OSTI ID: 1612533
- Grant/Contract Number:
- SC0016400
- Resource Type:
- Journal Article: Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Biotechnology & Applied Microbiology; Energy & Fuels; Camelina sativa; RNAi; Seed size; Seedling establishment; ADP-glucose pyrophosphorylase; Starch
Citation Formats
Na, GunNam, Aryal, Niranjan, Fatihi, Abdelhak, Kang, Jinling, and Lu, Chaofu. Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight. Netherlands: N. p., 2018.
Web. doi:10.1186/s13068-018-1334-2.
Na, GunNam, Aryal, Niranjan, Fatihi, Abdelhak, Kang, Jinling, & Lu, Chaofu. Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight. Netherlands. https://doi.org/10.1186/s13068-018-1334-2
Na, GunNam, Aryal, Niranjan, Fatihi, Abdelhak, Kang, Jinling, and Lu, Chaofu. 2018.
"Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight". Netherlands. https://doi.org/10.1186/s13068-018-1334-2.
@article{osti_1618745,
title = {Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight},
author = {Na, GunNam and Aryal, Niranjan and Fatihi, Abdelhak and Kang, Jinling and Lu, Chaofu},
abstractNote = {Background: Camelina (Camelina sativa L.) is a promising oilseed crop that may provide sustainable feedstock for biofuel production. One of the major drawbacks of Camelina is its smaller seeds compared to other major oil crops such as canola, which limit oil yield and may also pose challenges in successful seedling establishment, especially in dryland cultivation. Previous studies indicate that seed development may be under metabolic control. In oilseeds, starch only accumulates temporarily during seed development but is almost absent in mature seeds. In this study, we explored the efect of altering seed carbohydrate metabolism on Camelina seed size through down-regulating ADPglucose pyrophosphorylase (AGPase), a major enzyme in starch biosynthesis. Results: An RNAi construct comprising sequences of the Camelina small subunit of an AGPase (CsAPS) was expressed in Camelina cultivar Suneson under a seed-specifc promoter. The RNAi suppression reduced AGPase activities which concurred with moderately decreased starch accumulation during seed development. Transcripts of genes examined that are involved in storage products were not afected, but contents of sugars and water were increased in developing seeds. The transgenic seeds were larger than wild-type plants due to increased cell sizes in seed coat and embryos, and mature seeds contained similar oil but more protein contents. The larger seeds showed advantages on seedling emergence from deep soils. Conclusions: Changing starch and sugar metabolism during seed development may increase the size and mass of seeds without afecting their fnal oil content in Camelina. Increased seed size may improve seedling establishment in the feld and increase seed yield.},
doi = {10.1186/s13068-018-1334-2},
url = {https://www.osti.gov/biblio/1618745},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 11,
place = {Netherlands},
year = {Tue Dec 18 00:00:00 EST 2018},
month = {Tue Dec 18 00:00:00 EST 2018}
}
Web of Science
Works referenced in this record:
Signaling pathways of seed size control in plants
journal, October 2016
- Li, Na; Li, Yunhai
- Current Opinion in Plant Biology, Vol. 33
Seed-Development Programs: A Systems Biology–Based Comparison Between Dicots and Monocots
journal, April 2013
- Sreenivasulu, Nese; Wobus, Ulrich
- Annual Review of Plant Biology, Vol. 64, Issue 1
Seed Development and Differentiation: A Role for Metabolic Regulation
journal, July 2004
- Borisjuk, L.; Rolletschek, H.; Radchuk, R.
- Plant Biology, Vol. 6, Issue 4
Camelina uses, genetics, genomics, production, and management
journal, December 2016
- Berti, Marisol; Gesch, Russ; Eynck, Christina
- Industrial Crops and Products, Vol. 94
The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves
journal, September 2006
- Rosti, S.; Rudi, H.; Rudi, K.
- Journal of Experimental Botany, Vol. 57, Issue 14
Camelina Yield Response to Different Plant Populations under Dryland Conditions
journal, July 2011
- McVay, K. A.; Khan, Q. A.
- Agronomy Journal, Vol. 103, Issue 4
Influence of genotype and sowing date on camelina growth and yield in the north central U.S.
journal, March 2014
- Gesch, Russ W.
- Industrial Crops and Products, Vol. 54
The Genetic Basis of Natural Variation in Seed Size and Seed Number and Their Trade-Off Using Arabidopsis thaliana MAGIC Lines
journal, October 2014
- Gnan, Sebastian; Priest, Anne; Kover, Paula X.
- Genetics, Vol. 198, Issue 4
Endosperm turgor pressure decreases during early Arabidopsis seed development
journal, June 2016
- Beauzamy, Léna; Fourquin, Chloé; Dubrulle, Nelly
- Development, Vol. 143, Issue 18
SmartGrain : High-Throughput Phenotyping Software for Measuring Seed Shape through Image Analysis
journal, October 2012
- Tanabata, Takanari; Shibaya, Taeko; Hori, Kiyosumi
- Plant Physiology, Vol. 160, Issue 4
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter
journal, May 2007
- Vigeolas, Helene; Waldeck, Peter; Zank, Thorsten
- Plant Biotechnology Journal, Vol. 5, Issue 3
Carbon flux and fatty acid synthesis in plants
journal, March 2002
- Rawsthorne, Stephen
- Progress in Lipid Research, Vol. 41, Issue 2
An integrated overview of seed development in Arabidopsis thaliana ecotype WS
journal, February 2002
- Baud, Sébastien; Boutin, Jean-Pierre; Miquel, Martine
- Plant Physiology and Biochemistry, Vol. 40, Issue 2, p. 151-160
Evaluating Agronomic Responses of Camelina to Seeding Date under Rain-Fed Conditions
journal, January 2016
- Sintim, Henry Y.; Zheljazkov, Valtcho D.; Obour, Augustine K.
- Agronomy Journal, Vol. 108, Issue 1
The nature of selection during plant domestication
journal, February 2009
- Purugganan, Michael D.; Fuller, Dorian Q.
- Nature, Vol. 457, Issue 7231
Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013
- Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram
- Plant Biotechnology Journal, Vol. 11, Issue 6
Module-specific regulation of the β-phaseolin promoter during embryogenesis
journal, February 2003
- Chandrasekharan, Mahesh B.; Bishop, Kenneth J.; Hall, Timothy C.
- The Plant Journal, Vol. 33, Issue 5
Did greater burial depth increase the seed size of domesticated legumes?
journal, September 2013
- Kluyver, Thomas A.; Charles, Michael; Jones, Glynis
- Journal of Experimental Botany, Vol. 64, Issue 13
Networks controlling seed size in Arabidopsis
journal, February 2015
- Orozco-Arroyo, Gregorio; Paolo, Dario; Ezquer, Ignacio
- Plant Reproduction, Vol. 28, Issue 1
Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition
journal, May 2014
- Laurens, Lieve M. L.; Van Wychen, Stefanie; McAllister, Jordan P.
- Analytical Biochemistry, Vol. 452
Seedling survival and seed size: a synthesis of the literature
journal, May 2004
- Moles, Angela T.; Westoby, Mark
- Journal of Ecology, Vol. 92, Issue 3
Review on Effect of Seed Size on Seedling Vigour and Seed Yield
journal, February 2014
- Ambika, S.; Manonmani, V.; Somasundar, G.
- Research Journal of Seed Science, Vol. 7, Issue 2
Starch turnover in developing oilseed embryos
journal, June 2010
- Andriotis, Vasilios M. E.; Pike, Marilyn J.; Kular, Baldeep
- New Phytologist, Vol. 187, Issue 3
Oil-seed crop: Camelina sativa
journal, May 1997
- Zubr, Josef
- Industrial Crops and Products, Vol. 6, Issue 2
The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme
journal, January 1990
- Bhattacharyya, Madan K.; Smith, Alison M.; Ellis, T. H. Noel
- Cell, Vol. 60, Issue 1
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007
- Lu, Chaofu; Kang, Jinling
- Plant Cell Reports, Vol. 27, Issue 2
Embryo-Specific Reduction of ADP-Glc Pyrophosphorylase Leads to an Inhibition of Starch Synthesis and a Delay in Oil Accumulation in Developing Seeds of Oilseed Rape
journal, August 2004
- Vigeolas, Helene; Möhlmann, Torsten; Martini, Norbert
- Plant Physiology, Vol. 136, Issue 1
A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa
journal, July 2014
- Snapp, Anna R.; Kang, Jinling; Qi, Xiaoli
- Planta, Vol. 240, Issue 3
A Cascade of Sequentially Expressed Sucrose Transporters in the Seed Coat and Endosperm Provides Nutrition for the Arabidopsis Embryo
journal, March 2015
- Chen, Li-Qing; Lin, I. Winnie; Qu, Xiao-Qing
- The Plant Cell, Vol. 27, Issue 3
Arabidopsis sucrose synthase 2 and 3 modulate metabolic homeostasis and direct carbon towards starch synthesis in developing seeds
journal, June 2010
- Angeles-Núñez, Juan Gabriel; Tiessen, Axel
- Planta, Vol. 232, Issue 3
The Transport of Sugars to Developing Embryos Is Not via the Bulk Endosperm in Oilseed Rape Seeds
journal, June 2008
- Morley-Smith, Edward R.; Pike, Marilyn J.; Findlay, Kim
- Plant Physiology, Vol. 147, Issue 4
The Effect of Genetically Based Differences in seed size on Seedling Survival in Arabidopsis Thaliana (Brassicaceae)
journal, March 1991
- Krannitz, Pam G.; Aarssen, Lonnie W.; Dow, Jennifer M.
- American Journal of Botany, Vol. 78, Issue 3
Dynamic Analyses of the Expression of the HISTONE::YFP Fusion Protein in Arabidopsis Show That Syncytial Endosperm Is Divided in Mitotic Domains
journal, March 2001
- Boisnard-Lorig, Corinne; Colon-Carmona, Adan; Bauch, Marion
- The Plant Cell, Vol. 13, Issue 3
Stem carbohydrate dynamics and expression of genes involved in fructan accumulation and remobilization during grain growth in wheat (Triticum aestivum L.) genotypes with contrasting tolerance to water stress
journal, May 2017
- Yáñez, Alejandra; Tapia, Gerardo; Guerra, Fernando
- PLOS ONE, Vol. 12, Issue 5
A WRKY Transcription Factor Recruits the SYG1-Like Protein SHB1 to Activate Gene Expression and Seed Cavity Enlargement
journal, March 2013
- Kang, Xiaojun; Li, Wei; Zhou, Yun
- PLoS Genetics, Vol. 9, Issue 3
Antisense-inhibition of ADP-glucose pyrophosphorylase in developing seeds of Vicia narbonensis moderately decreases starch but increases protein content and affects seed maturation
journal, October 2000
- Weber, Hans; Rolletschek, Hardy; Heim, Ute
- The Plant Journal, Vol. 24, Issue 1
Early embryogenesis in Arabidopsis thaliana . II. The developing embryo
journal, March 1991
- Mansfield, S. G.; Briarty, L. G.
- Canadian Journal of Botany, Vol. 69, Issue 3
Camelina sativa as a fallow replacement crop in wheat-based crop production systems in the US Great Plains
journal, January 2018
- Obour, Augustine K.; Chen, Chengci; Sintim, Henry Y.
- Industrial Crops and Products, Vol. 111
The plasma membrane–bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana
journal, March 2004
- Li, Weiqi; Li, Maoyin; Zhang, Wenhua
- Nature Biotechnology, Vol. 22, Issue 4