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Title: Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A

Abstract

Here, the model yeast, Saccharomyces cerevisiae, is not known to be oleaginous. However, an industrial wild-type strain, D5A, was shown to accumulate over 20% storage lipids from glucose when growth is nitrogen-limited compared to no more than 7% lipid accumulation without nitrogen stress. To elucidate the mechanisms of S. cerevisiae D5A oleaginicity, we compared physiological and metabolic changes; as well as the transcriptional profiles of the oleaginous industrial strain, D5A, and a non-oleaginous laboratory strain, BY4741, under normal and nitrogen-limited conditions using analytic techniques and next-generation sequencing-based RNA-Seq transcriptomics. Transcriptional levels for genes associated with fatty acid biosynthesis, nitrogen metabolism, amino acid catabolism, as well as the pentose phosphate pathway and ethanol oxidation in central carbon (C) metabolism, were up-regulated in D5A during nitrogen deprivation. Despite increased carbon flux to lipids, most gene-encoding enzymes involved in triacylglycerol (TAG) assembly were expressed at similar levels regardless of the varying nitrogen concentrations in the growth media and strain backgrounds. Phospholipid turnover also contributed to TAG accumulation through increased precursor production with the down-regulation of subsequent phospholipid synthesis steps. Our results also demonstrated that nitrogen assimilation via the glutamate-glutamine pathway and amino acid metabolism, as well as the fluxes of carbon and reductantsmore » from central C metabolism, are integral to the general oleaginicity of D5A, which resulted in the enhanced lipid storage during nitrogen deprivation. This work demonstrated the disequilibrium and rebalance of carbon and nitrogen contribution to the accumulation of lipids in the oleaginous yeast S. cerevisiae D5A. Rather than TAG assembly from acyl groups, the major switches for the enhanced lipid accumulation of D5A (i.e., fatty acid biosynthesis) are the increases of cytosolic pools of acetyl-CoA and NADPH, as well as alternative nitrogen assimilation.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1618737
Alternate Identifier(s):
OSTI ID: 1476705
Report Number(s):
NREL/JA-5100-71803
Journal ID: ISSN 1754-6834; 258; PII: 1256
Grant/Contract Number:  
AC36-08GO28308; 2018ACA149
Resource Type:
Journal Article: Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; oleaginous yeast; Saccharomyces cerevisiae; transcriptomics; RNA-Seq; lipid accumulation; triacylglycerol (TAG); nitrogen assimilation

Citation Formats

He, Qiaoning, Yang, Yongfu, Yang, Shihui, Donohoe, Bryon S., Van Wychen, Stefanie, Zhang, Min, Himmel, Michael E., and Knoshaug, Eric P. Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A. Netherlands: N. p., 2018. Web. doi:10.1186/s13068-018-1256-z.
He, Qiaoning, Yang, Yongfu, Yang, Shihui, Donohoe, Bryon S., Van Wychen, Stefanie, Zhang, Min, Himmel, Michael E., & Knoshaug, Eric P. Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A. Netherlands. doi:10.1186/s13068-018-1256-z.
He, Qiaoning, Yang, Yongfu, Yang, Shihui, Donohoe, Bryon S., Van Wychen, Stefanie, Zhang, Min, Himmel, Michael E., and Knoshaug, Eric P. Mon . "Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A". Netherlands. doi:10.1186/s13068-018-1256-z.
@article{osti_1618737,
title = {Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A},
author = {He, Qiaoning and Yang, Yongfu and Yang, Shihui and Donohoe, Bryon S. and Van Wychen, Stefanie and Zhang, Min and Himmel, Michael E. and Knoshaug, Eric P.},
abstractNote = {Here, the model yeast, Saccharomyces cerevisiae, is not known to be oleaginous. However, an industrial wild-type strain, D5A, was shown to accumulate over 20% storage lipids from glucose when growth is nitrogen-limited compared to no more than 7% lipid accumulation without nitrogen stress. To elucidate the mechanisms of S. cerevisiae D5A oleaginicity, we compared physiological and metabolic changes; as well as the transcriptional profiles of the oleaginous industrial strain, D5A, and a non-oleaginous laboratory strain, BY4741, under normal and nitrogen-limited conditions using analytic techniques and next-generation sequencing-based RNA-Seq transcriptomics. Transcriptional levels for genes associated with fatty acid biosynthesis, nitrogen metabolism, amino acid catabolism, as well as the pentose phosphate pathway and ethanol oxidation in central carbon (C) metabolism, were up-regulated in D5A during nitrogen deprivation. Despite increased carbon flux to lipids, most gene-encoding enzymes involved in triacylglycerol (TAG) assembly were expressed at similar levels regardless of the varying nitrogen concentrations in the growth media and strain backgrounds. Phospholipid turnover also contributed to TAG accumulation through increased precursor production with the down-regulation of subsequent phospholipid synthesis steps. Our results also demonstrated that nitrogen assimilation via the glutamate-glutamine pathway and amino acid metabolism, as well as the fluxes of carbon and reductants from central C metabolism, are integral to the general oleaginicity of D5A, which resulted in the enhanced lipid storage during nitrogen deprivation. This work demonstrated the disequilibrium and rebalance of carbon and nitrogen contribution to the accumulation of lipids in the oleaginous yeast S. cerevisiae D5A. Rather than TAG assembly from acyl groups, the major switches for the enhanced lipid accumulation of D5A (i.e., fatty acid biosynthesis) are the increases of cytosolic pools of acetyl-CoA and NADPH, as well as alternative nitrogen assimilation.},
doi = {10.1186/s13068-018-1256-z},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 11,
place = {Netherlands},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1186/s13068-018-1256-z

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Cited by: 2 works
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