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Title: Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica

Abstract

Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels. In this study, we successfully demonstrate the production of fatty alcohols by the oleaginous yeasts, Yarrowia lipolytica and Lipomyces starkeyi, through expression of the fatty acyl-CoA reductase gene from Marinobactor aquaeolei VT8. This strategy resulted in the production of 167 and 770 mg/L of fatty alcohols in shake flask from Y. lipolytica and L starkeyi, respectively. When using a dodecane overlay during fermentation, 92 and 99% of total fatty alcohols produced by Y. lipolytica and L. starkeyi, respectively, were extracted into the dodecane phase, which compares favorably to the 3 and 50% recovered, respectively, without the dodecane layer. In both oleaginous yeasts, long chain length, saturated fatty alcohols, i.e., hexadecanol (C16:0) and octadecanol (C18:0), were predominant and accounted for more thanmore » 85% of the total fatty alcohols produced. To the best of our knowledge, this is the first report of fatty alcohol production in L. starkeyi. Furthermore, this work demonstrates that the oleaginous yeasts, Y. lipolytica and L. starkeyi, can serve as platform organisms for the production of fatty acid-derived biofuels and bioproducts.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1618653
Alternate Identifier(s):
OSTI ID: 1332481
Report Number(s):
NREL/JA-2700-65740
Journal ID: ISSN 1754-6834; 227; PII: 647
Grant/Contract Number:  
AC36-08-GO28308; AC36-08GO28308
Resource Type:
Journal Article: Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; metabolic engineering; oleaginous yeasts; Yarrowia lipolytica; Lipomyces starkeyi; fatty alcohols

Citation Formats

Wang, Wei, Wei, Hui, Knoshaug, Eric, Van Wychen, Stefanie, Xu, Qi, Himmel, Michael E., and Zhang, Min. Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica. Netherlands: N. p., 2016. Web. doi:10.1186/s13068-016-0647-2.
Wang, Wei, Wei, Hui, Knoshaug, Eric, Van Wychen, Stefanie, Xu, Qi, Himmel, Michael E., & Zhang, Min. Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica. Netherlands. https://doi.org/10.1186/s13068-016-0647-2
Wang, Wei, Wei, Hui, Knoshaug, Eric, Van Wychen, Stefanie, Xu, Qi, Himmel, Michael E., and Zhang, Min. 2016. "Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica". Netherlands. https://doi.org/10.1186/s13068-016-0647-2.
@article{osti_1618653,
title = {Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica},
author = {Wang, Wei and Wei, Hui and Knoshaug, Eric and Van Wychen, Stefanie and Xu, Qi and Himmel, Michael E. and Zhang, Min},
abstractNote = {Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels. In this study, we successfully demonstrate the production of fatty alcohols by the oleaginous yeasts, Yarrowia lipolytica and Lipomyces starkeyi, through expression of the fatty acyl-CoA reductase gene from Marinobactor aquaeolei VT8. This strategy resulted in the production of 167 and 770 mg/L of fatty alcohols in shake flask from Y. lipolytica and L starkeyi, respectively. When using a dodecane overlay during fermentation, 92 and 99% of total fatty alcohols produced by Y. lipolytica and L. starkeyi, respectively, were extracted into the dodecane phase, which compares favorably to the 3 and 50% recovered, respectively, without the dodecane layer. In both oleaginous yeasts, long chain length, saturated fatty alcohols, i.e., hexadecanol (C16:0) and octadecanol (C18:0), were predominant and accounted for more than 85% of the total fatty alcohols produced. To the best of our knowledge, this is the first report of fatty alcohol production in L. starkeyi. Furthermore, this work demonstrates that the oleaginous yeasts, Y. lipolytica and L. starkeyi, can serve as platform organisms for the production of fatty acid-derived biofuels and bioproducts.},
doi = {10.1186/s13068-016-0647-2},
url = {https://www.osti.gov/biblio/1618653}, journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 9,
place = {Netherlands},
year = {Mon Oct 24 00:00:00 EDT 2016},
month = {Mon Oct 24 00:00:00 EDT 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1186/s13068-016-0647-2

Citation Metrics:
Cited by: 43 works
Citation information provided by
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Works referenced in this record:

Fatty alcohol production in engineered E. coli expressing Marinobacter fatty acyl-CoA reductases
journal, June 2013


Laboratory evolution of copper tolerant yeast strains
journal, January 2012


Genome evolution in yeasts
journal, July 2004


Production of medium chain length fatty alcohols from glucose in Escherichia coli
journal, November 2013


Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients
journal, April 2011


Transcriptomic Analyses during the Transition from Biomass Production to Lipid Accumulation in the Oleaginous Yeast Yarrowia lipolytica
journal, November 2011


Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production
journal, January 2015


Vectors for gene expression and amplification in the yeastYarrowia lipolytica
journal, January 2001


Heterologous Expression of Xylanase Enzymes in Lipogenic Yeast Yarrowia lipolytica
journal, December 2014


Oligomerization of Ethylene to α-Olefins: Discovery and Development of the Shell Higher Olefin Process (SHOP)
journal, October 2013


Integrative transformation of the yeast Yarrowia lipolytica
journal, October 1985


Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
journal, January 2010


Fatty alcohols production by oleaginous yeast
journal, August 2015


Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering
journal, May 2015


An optimized transformation protocol for Lipomyces starkeyi
journal, April 2014


Physiology and genetics of the dimorphic fungus Yarrowia lipolytica
journal, April 1997


Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeastLipomyces starkeyi
journal, May 2008


Development of an orthogonal fatty acid biosynthesis system in E. coli for oleochemical production
journal, July 2015


Techno-economic evaluation of integrated first- and second-generation ethanol production from grain and straw
journal, January 2016


Unraveling fatty acid transport and activation mechanisms in Yarrowia lipolytica
journal, September 2015


Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production
journal, January 2014


Oleaginous yeasts for biodiesel: Current and future trends in biology and production
journal, November 2014


Metabolic engineering of Yarrowia lipolytica for industrial applications
journal, December 2015


Works referencing / citing this record:

A metabolic engineering strategy for producing free fatty acids by the Yarrowia lipolytica yeast based on impairment of glycerol metabolism
journal, November 2017


Lipomyces starkeyi: an emerging cell factory for production of lipids, oleochemicals and biotechnology applications
journal, September 2018


Metabolism of aromatics by Trichosporon oleaginosus while remaining oleaginous
journal, November 2017


Increasing medium chain fatty acids production in Yarrowia lipolytica by metabolic engineering
journal, September 2018


A GFP-fusion coupling FACS platform for advancing the metabolic engineering of filamentous fungi
journal, August 2018


Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
journal, September 2018


Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals
journal, November 2017