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Title: Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine

Abstract

In order to investigate host factors associated with the establishment of persistent foot-and-mouth disease virus (FMDV) infection, the systemic response to vaccination and challenge was studied in 47 steers. Eighteen steers that had received a recombinant FMDV A vaccine 2 weeks earlier and 29 non-vaccinated steers were challenged by intra-nasopharyngeal deposition of FMDV A24. For up to 35 days after challenge, host factors including complete blood counts with T lymphocyte subsets, type I/III interferon (IFN) activity, neutralizing and total FMDV-specific antibody titers in serum, as well as antibody-secreting cells (in 6 non-vaccinated animals) were characterized in the context of viral infection dynamics. As a result, vaccination generally induced a strong antibody response. There was a transient peak of FMDV-specific serum IgM in non-vaccinated animals after challenge, while IgM levels in vaccinated animals did not increase further. Both groups had a lasting increase of specific IgG and neutralizing antibody after challenge. Substantial systemic IFN activity in non-vaccinated animals coincided with viremia, and no IFN or viremia was detected in vaccinated animals. After challenge, circulating lymphocytes decreased in non-vaccinated animals, coincident with viremia, IFN activity, and clinical disease, whereas lymphocyte and monocyte counts in vaccinated animals were unaffected by vaccination but transientlymore » increased after challenge. The CD4 +/CD8 + T cell ratio in non-vaccinated animals increased during acute infection, driven by an absolute decrease of CD8 + cells. In conclusion, the incidence of FMDV persistence was 61.5 % in non-vaccinated and 54.5 % in vaccinated animals. Overall, the systemic factors examined were not associated with the FMDV carrier/non-carrier divergence; however, significant differences were identified between responses of non-vaccinated and vaccinated cattle.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1618646
Alternate Identifier(s):
OSTI ID: 1375812
Resource Type:
Journal Article: Published Article
Journal Name:
BMC Veterinary Research
Additional Journal Information:
Journal Name: BMC Veterinary Research Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1746-6148
Publisher:
Springer Science + Business Media
Country of Publication:
United Kingdom
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; FMD; Vaccination; Persistence; Carrier; Flow; cytometry; Lymphopenia; Interferon; ELISA; ELISPOT

Citation Formats

Eschbaumer, Michael, Stenfeldt, Carolina, Rekant, Steven I., Pacheco, Juan M., Hartwig, Ethan J., Smoliga, George R., Kenney, Mary A., Golde, William T., Rodriguez, Luis L., and Arzt, Jonathan. Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine. United Kingdom: N. p., 2016. Web. doi:10.1186/s12917-016-0838-x.
Eschbaumer, Michael, Stenfeldt, Carolina, Rekant, Steven I., Pacheco, Juan M., Hartwig, Ethan J., Smoliga, George R., Kenney, Mary A., Golde, William T., Rodriguez, Luis L., & Arzt, Jonathan. Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine. United Kingdom. https://doi.org/10.1186/s12917-016-0838-x
Eschbaumer, Michael, Stenfeldt, Carolina, Rekant, Steven I., Pacheco, Juan M., Hartwig, Ethan J., Smoliga, George R., Kenney, Mary A., Golde, William T., Rodriguez, Luis L., and Arzt, Jonathan. Thu . "Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine". United Kingdom. https://doi.org/10.1186/s12917-016-0838-x.
@article{osti_1618646,
title = {Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naïve cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine},
author = {Eschbaumer, Michael and Stenfeldt, Carolina and Rekant, Steven I. and Pacheco, Juan M. and Hartwig, Ethan J. and Smoliga, George R. and Kenney, Mary A. and Golde, William T. and Rodriguez, Luis L. and Arzt, Jonathan},
abstractNote = {In order to investigate host factors associated with the establishment of persistent foot-and-mouth disease virus (FMDV) infection, the systemic response to vaccination and challenge was studied in 47 steers. Eighteen steers that had received a recombinant FMDV A vaccine 2 weeks earlier and 29 non-vaccinated steers were challenged by intra-nasopharyngeal deposition of FMDV A24. For up to 35 days after challenge, host factors including complete blood counts with T lymphocyte subsets, type I/III interferon (IFN) activity, neutralizing and total FMDV-specific antibody titers in serum, as well as antibody-secreting cells (in 6 non-vaccinated animals) were characterized in the context of viral infection dynamics. As a result, vaccination generally induced a strong antibody response. There was a transient peak of FMDV-specific serum IgM in non-vaccinated animals after challenge, while IgM levels in vaccinated animals did not increase further. Both groups had a lasting increase of specific IgG and neutralizing antibody after challenge. Substantial systemic IFN activity in non-vaccinated animals coincided with viremia, and no IFN or viremia was detected in vaccinated animals. After challenge, circulating lymphocytes decreased in non-vaccinated animals, coincident with viremia, IFN activity, and clinical disease, whereas lymphocyte and monocyte counts in vaccinated animals were unaffected by vaccination but transiently increased after challenge. The CD4+/CD8+ T cell ratio in non-vaccinated animals increased during acute infection, driven by an absolute decrease of CD8+ cells. In conclusion, the incidence of FMDV persistence was 61.5 % in non-vaccinated and 54.5 % in vaccinated animals. Overall, the systemic factors examined were not associated with the FMDV carrier/non-carrier divergence; however, significant differences were identified between responses of non-vaccinated and vaccinated cattle.},
doi = {10.1186/s12917-016-0838-x},
url = {https://www.osti.gov/biblio/1618646}, journal = {BMC Veterinary Research},
issn = {1746-6148},
number = 1,
volume = 12,
place = {United Kingdom},
year = {2016},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1186/s12917-016-0838-x

Citation Metrics:
Cited by: 8 works
Citation information provided by
Web of Science

Figures / Tables:

Fig. 1 Fig. 1: Overview of the experimental timeline. Each bar represents one animal, with the length of the bar corresponding to the time the animal remained in the experiment after challenge infection (21 dpi, the revised cut-off by which FMDV persistence status could be determined, is marked by a vertical line).more » Non-vaccinated animals are shown in red, vaccinated animals in blue. Solid-colored bars are FMDV carriers, unfilled bars are non-carriers. Striped bars are used when the persistence status of an animal could not be determined because it was euthanized before 21 dpi. Asterisks mark the three animals without convincing evidence of infection (see Results section for details)« less

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    Works referencing / citing this record:

    Model of persistent foot‐and‐mouth disease virus infection in multilayered cells derived from bovine dorsal soft palate
    journal, August 2019


    Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection
    journal, April 2017


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    journal, November 2018


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.