Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification

Chung, Daehwan; Verbeke, Tobin J.; Cross, Karissa L.; Westpheling, Janet; Elkins, James G.

doi:10.1186/s13068-015-0287-y

Title: Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification

Journal Article · Wed Jul 22 00:00:00 EDT 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0287-y· OSTI ID:1618614

Chung, Daehwan; Verbeke, Tobin J.; Cross, Karissa L.; Westpheling, Janet; Elkins, James G.

Compounds such as furfural and 5-hydroxymethylfurfural (5-HMF) are generated through the dehydration of xylose and glucose, respectively, during dilute-acid pretreatment of lignocellulosic biomass and are also potent microbial growth and fermentation inhibitors. The enzymatic reduction of these furan aldehydes to their corresponding, and less toxic, alcohols is an engineering approach that has been successfully implemented in both Saccharomyces cerevisiae and ethanologenicEscherichia coli, but has not yet been investigated in thermophiles relevant to biofuel production through consolidated bioprocessing (CBP). Developing CBP-relevant biocatalysts that are either naturally resistant to such inhibitors, or are amenable to engineered resistance, is therefore, an important component in making biofuels production from lignocellulosic biomass feasible.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: PS02-717 06ER64304; AC05-00OR22725

OSTI ID:: 1618614

Alternate ID(s):: OSTI ID: 1201292

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 8 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 13 works

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Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance Kim, Sun-Ki; Groom, Joseph; Chung, Daehwan Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0750-z	journal	March 2017
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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Thermophile
Lignocellulose
Biofuel
Furfural
5-hydroxymethylfurfural
Inhibitor
Genetic engineering
Caldicellulosiruptor bescii

Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification [Supplemental Data] Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441 The current record is supplemented by this figure	figure	July 2015
Additional file 1: of Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441_d1 The current record is supplemented by this text	text	January 2015
Additional file 1: of Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441_d1.v1 The current record is supplemented by this figure	figure	July 2015
Additional file 2: of Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441_d2 The current record is supplemented by this text	text	January 2015
Additional file 2: of Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441_d2.v1 The current record is supplemented by this dataset	dataset	July 2015
Additional file 3: of Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441_d3 The current record is supplemented by this text	text	January 2015
Additional file 3: of Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification Chung, Daehwan; Verbeke, Tobin; Cross, Karissa https://doi.org/10.6084/m9.figshare.c.3608441_d3.v1 The current record is supplemented by this dataset	dataset	July 2015
Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification [Supplemental Data] Verbeke, Tobin; Cross, Karissa; Westpheling, Janet https://doi.org/10.6084/m9.figshare.c.3608441.v1 The current record is supplemented by this collection	collection	July 2015