Regulation of Nitrogen Metabolism by GATA Zinc Finger Transcription Factors in Yarrowia lipolytica

Pomraning, Kyle R.; Bredeweg, Erin L.; Baker, Scott E.; Mitchell, ed., Aaron P.

doi:10.1128/mSphere.00038-17

Title: Regulation of Nitrogen Metabolism by GATA Zinc Finger Transcription Factors in Yarrowia lipolytica

Journal Article · Wed Feb 22 00:00:00 EST 2017 · mSphere

DOI:https://doi.org/10.1128/mSphere.00038-17· OSTI ID:1618365

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^[2]; Mitchell, ed., Aaron P.

Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA

Here, fungi accumulate lipids in a manner dependent on the quantity and quality of the nitrogen source on which they are growing. In the oleaginous yeast Yarrowia lipolytica, growth on a complex source of nitrogen enables rapid growth and limited accumulation of neutral lipids, while growth on a simple nitrogen source promotes lipid accumulation in large lipid droplets. Here we examined the roles of nitrogen catabolite repression and its regulation by GATA zinc finger transcription factors on lipid metabolism in Y. lipolytica. Deletion of the GATA transcription factor genes gzf3 and gzf2 resulted in nitrogen source-specific growth defects and greater accumulation of lipids when the cells were growing on a simple nitrogen source. Deletion of gzf1, which is most similar to activators of genes repressed by nitrogen catabolite repression in filamentous ascomycetes, did not affect growth on the nitrogen sources tested. We examined gene expression of wild-type and GATA transcription factor mutants on simple and complex nitrogen sources and found that expression of enzymes involved in malate metabolism, beta-oxidation, and ammonia utilization are strongly upregulated on a simple nitrogen source. Deletion of gzf3 results in overexpression of genes with GATAA sites in their promoters, suggesting that it acts as a repressor, while gzf2 is required for expression of ammonia utilization genes but does not grossly affect the transcription level of genes predicted to be controlled by nitrogen catabolite repression. Both GATA transcription factor mutants exhibit decreased expression of genes controlled by carbon catabolite repression via the repressor mig1, including genes for beta-oxidation, highlighting the complex interplay between regulation of carbon, nitrogen, and lipid metabolism.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0008744

OSTI ID:: 1618365

Alternate ID(s):: OSTI ID: 1366736

Journal Information:: mSphere, Journal Name: mSphere Vol. 2 Journal Issue: 1; ISSN 2379-5042

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 25 works

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59 BASIC BIOLOGICAL SCIENCES
biotechnology
carbon metabolism
lipid synthesis
metabolic regulation
nitrogen metabolism
yeasts
Yarrowia lipolytica
nitrogen catabolite repression
carbon catabolite repression
zinc finger
transcription factor
GATA transcription factor
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nitrogen
gene regulation
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lipid metabolism

Title: Regulation of Nitrogen Metabolism by GATA Zinc Finger Transcription Factors in Yarrowia lipolytica

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