Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg

Hanifzadeh, MohammadMatin; Garcia, Elena Cerdan; Viamajala, Sridhar

doi:10.1016/j.renene.2018.05.012

Title: Production of lipid and carbohydrate from microalgae without compromising biomass productivities: Role of Ca and Mg

Journal Article · Sat May 05 00:00:00 EDT 2018 · Renewable Energy

DOI:https://doi.org/10.1016/j.renene.2018.05.012· OSTI ID:1613321

^[1]; Garcia, Elena Cerdan ^[2];

^[1]

Univ. of Toledo, OH (United States)
Univ. of Toledo, OH (United States); Univ. of Southampton (United Kingdom)

We report the cultivation of Chlorella sorokiniana str. SLA-04 in media containing trace amounts of Ca and Mg. The differences in productivities of biomass, lipids and carbohydrates were assessed relative to cultures grown in standard media (BG-11) that contain approximately 8 × higher concentration of Ca and 30 × higher concentration of Mg. Culture performance in N-limited standard media was also investigated. In addition to growth and accumulation of storage products (lipid and carbohydrate), we measured the utilization of N, Ca and Mg and monitored changes in cell size and photosynthetic activity. Our results showed that limitation of Ca or Mg did not inhibit cell replication and culture growth. On the contrary, Ca-limited (Ca^L) cultures had ~30% higher biomass productivity relative to the control with excessive nutrients possibly due to improvement in cell wall flexibility and cell division. We also observed that Ca^L and Mg-limited (Mg^L) cultures had nearly 3-fold higher lipid concentration (measured as fatty acid methyl ester) and 50% higher carbohydrate concentration than the nutrient excess control cultures. Simultaneous culture growth and lipid accumulation in Ca^L and Mg^L cultures suggest that de novo synthesis was the primary mechanism for lipid accumulation in Ca/Mg-limited media. Overall, our study demonstrates that micronutrient optimization, in addition to optimization of macronutrients, could significantly improve microalgal biorefinery yields.

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Research Organization:: Univ. of Toledo, OH (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE

Grant/Contract Number:: EE0005993

OSTI ID:: 1613321

Alternate ID(s):: OSTI ID: 1548192

Journal Information:: Renewable Energy, Vol. 127; ISSN 0960-1481

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 44 works

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References (46)

High-value products from microalgae—their development and commercialisation Borowitzka, Michael A. Journal of Applied Phycology, Vol. 25, Issue 3 https://doi.org/10.1007/s10811-013-9983-9	journal	January 2013
Production and harvesting of microalgae for wastewater treatment, biofuels, and bioproducts Christenson, Logan; Sims, Ronald Biotechnology Advances, Vol. 29, Issue 6, p. 686-702 https://doi.org/10.1016/j.biotechadv.2011.05.015	journal	November 2011
A Novel Approach to Improve the Barrier Properties of PET/Clay Nanocomposites Majdzadeh-Ardakani, Kazem; Zekriardehani, Shahab; Coleman, Maria R. International Journal of Polymer Science, Vol. 2017 https://doi.org/10.1155/2017/7625906	journal	January 2017
Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus Arumugam, Muthu; Agarwal, Ankur; Arya, Mahesh Chandra Bioresource Technology, Vol. 131 https://doi.org/10.1016/j.biortech.2012.12.159	journal	March 2013
Cyanobacteria and microalgae: A positive prospect for biofuels Parmar, Asha; Singh, Niraj Kumar; Pandey, Ashok Bioresource Technology, Vol. 102, Issue 22 https://doi.org/10.1016/j.biortech.2011.08.030	journal	November 2011
Characterization of reverse osmosis and nanofiltration membranes: effects of operating conditions and specific ion rejection Vaseghi, Ghazaleh; Ghassemi, Abbas; Loya, Jim Desalination and Water Treatment, Vol. 57, Issue 50 https://doi.org/10.1080/19443994.2015.1135825	journal	January 2016
TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation Merchant, Sabeeha S.; Kropat, Janette; Liu, Bensheng Current Opinion in Biotechnology, Vol. 23, Issue 3 https://doi.org/10.1016/j.copbio.2011.12.001	journal	June 2012
Amino Acid Metabolism Miflin, B. J.; Lea, P. J. Annual Review of Plant Physiology, Vol. 28, Issue 1 https://doi.org/10.1146/annurev.pp.28.060177.001503	journal	June 1977
Understanding chlorophylls: Central magnesium ion and phytyl as structural determinants Fiedor, Leszek; Kania, Agnieszka; Myśliwa-Kurdziel, Beata Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1777, Issue 12 https://doi.org/10.1016/j.bbabio.2008.09.005	journal	December 2008
Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga— Penium margaritaceum : Live Cell Labeling with Monoclonal Antibodies Domozych, David S.; Brechka, Hannah; Britton, Alicia Journal of Botany, Vol. 2011 https://doi.org/10.1155/2011/632165	journal	February 2011
The effect of physical and chemical treatment on the mechanical properties of the cell wall of the alga Chara corallina Toole, Geraldine A.; Smith, Andrew C.; Waldron, Keith W. Planta, Vol. 214, Issue 3 https://doi.org/10.1007/s004250100641	journal	October 2001
Effect of Chain Dynamics, Crystallinity, and Free Volume on the Barrier Properties of Poly(ethylene terephthalate) Biaxially Oriented Films Zekriardehani, S.; Jabarin, S. A.; Gidley, D. R. Macromolecules, Vol. 50, Issue 7 https://doi.org/10.1021/acs.macromol.7b00198	journal	March 2017
Selective degradation of the recalcitrant cell wall of Scenedesmus quadricauda CASA CC202 Reshma, Ragini; Arumugam, Muthu Planta, Vol. 246, Issue 4 https://doi.org/10.1007/s00425-017-2732-6	journal	July 2017
Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa Solovchenko, A. E.; Khozin-Goldberg, I.; Didi-Cohen, S. Journal of Applied Phycology, Vol. 20, Issue 3 https://doi.org/10.1007/s10811-007-9233-0	journal	August 2007
Effect of Magnesium on Chlorophyll-Protein Complexes Aro, Eva-Mari; Valanne, Niina Physiologia Plantarum, Vol. 43, Issue 3 https://doi.org/10.1111/j.1399-3054.1978.tb02574.x	journal	July 1978
The Influence of Magnesium Deficiency, Chlorophyll Concentration, and heat Treatments on the rate of Photosynthesis of Chlorella Kennedy, Sidney R. American Journal of Botany, Vol. 27, Issue 2 https://doi.org/10.1002/j.1537-2197.1940.tb14218.x	journal	February 1940
Calcium at the Cell Wall-Cytoplast Interface Hepler, Peter K.; Winship, Lawrence J. Journal of Integrative Plant Biology, Vol. 52, Issue 2 https://doi.org/10.1111/j.1744-7909.2010.00923.x	journal	February 2010
The Effect of Magnesium Concentration on Chlorophyll and Catalase Development in Chlorella Finkle, Bernard J.; Appleman, David Plant Physiology, Vol. 28, Issue 4 https://doi.org/10.1104/pp.28.4.652	journal	October 1953
Optical properties of microalgae for enhanced biofuels production Mitra, Mautusi; Melis, Anastasios Optics Express, Vol. 16, Issue 26 https://doi.org/10.1364/OE.16.021807	journal	January 2008
Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2 Ruangsomboon, Suneerat Bioresource Technology, Vol. 109 https://doi.org/10.1016/j.biortech.2011.07.025	journal	April 2012
Effects of temperature and nutrient regimes on biomass and lipid production by six oleaginous microalgae in batch culture employing a two-phase cultivation strategy Roleda, Michael Y.; Slocombe, Stephen P.; Leakey, Raymond J. G. Bioresource Technology, Vol. 129 https://doi.org/10.1016/j.biortech.2012.11.043	journal	February 2013
Simultaneous growth and neutral lipid accumulation in microalgae Klok, Anne J.; Martens, Dirk E.; Wijffels, René H. Bioresource Technology, Vol. 134 https://doi.org/10.1016/j.biortech.2013.02.006	journal	April 2013
Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga Guarnieri, Michael T.; Nag, Ambarish; Smolinski, Sharon L. PLoS ONE, Vol. 6, Issue 10 https://doi.org/10.1371/journal.pone.0025851	journal	October 2011
The Response of Nannochloropsis gaditana to Nitrogen Starvation Includes De Novo Biosynthesis of Triacylglycerols, a Decrease of Chloroplast Galactolipids, and Reorganization of the Photosynthetic Apparatus Simionato, Diana; Block, Maryse A.; La Rocca, Nicoletta Eukaryotic Cell, Vol. 12, Issue 5 https://doi.org/10.1128/EC.00363-12	journal	March 2013
Physiological and molecular analysis of carbon source supplementation and pH stress-induced lipid accumulation in the marine diatom Phaeodactylum tricornutum Mus, Florence; Toussaint, Jean-Paul; Cooksey, Keith E. Applied Microbiology and Biotechnology, Vol. 97, Issue 8 https://doi.org/10.1007/s00253-013-4747-7	journal	March 2013
Microalgae for high-value compounds and biofuels production: A review with focus on cultivation under stress conditions Markou, Giorgos; Nerantzis, Elias Biotechnology Advances, Vol. 31, Issue 8 https://doi.org/10.1016/j.biotechadv.2013.07.011	journal	December 2013
Characterization of calcium deposition induced by Synechocystis sp. PCC6803 in BG11 culture medium Yan, Huaxiao; Han, Zuozhen; Zhao, Hui Chinese Journal of Oceanology and Limnology, Vol. 32, Issue 3 https://doi.org/10.1007/s00343-014-3150-2	journal	October 2013
Elemental balancing of biomass and medium composition enhances growth capacity in high-densityChlorella vulgaris cultures Mandalam, Ramkumar K.; Palsson, Bernhard Biotechnology and Bioengineering, Vol. 59, Issue 5 https://doi.org/10.1002/(SICI)1097-0290(19980905)59:5<605::AID-BIT11>3.0.CO;2-8	journal	September 1998
Cultivation of Microalgae at Extreme Alkaline pH Conditions: A Novel Approach for Biofuel Production Vadlamani, Agasteswar; Viamajala, Sridhar; Pendyala, Brahmaiah ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 8 https://doi.org/10.1021/acssuschemeng.7b01534	journal	July 2017
The environmental photobioreactor (ePBR): An algal culturing platform for simulating dynamic natural environments Lucker, Ben F.; Hall, Christopher C.; Zegarac, Robert Algal Research, Vol. 6 https://doi.org/10.1016/j.algal.2013.12.007	journal	October 2014
New Fluorescence Parameters for the Determination of Q _A Redox State and Excitation Energy Fluxes Kramer, David M.; Johnson, Giles; Kiirats, Olavi Photosynthesis Research, Vol. 79, Issue 2 https://doi.org/10.1023/B:PRES.0000015391.99477.0d	journal	January 2004
Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification Laurens, Lieve M. L.; Quinn, Matthew; Van Wychen, Stefanie Analytical and Bioanalytical Chemistry, Vol. 403, Issue 1 https://doi.org/10.1007/s00216-012-5814-0	journal	February 2012
Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169 Msanne, Joseph; Xu, Di; Konda, Anji Reddy Phytochemistry, Vol. 75 https://doi.org/10.1016/j.phytochem.2011.12.007	journal	March 2012
Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077 Pancha, Imran; Chokshi, Kaumeel; George, Basil Bioresource Technology, Vol. 156 https://doi.org/10.1016/j.biortech.2014.01.025	journal	March 2014
Regulation of calcium and magnesium homeostasis in plants: from transporters to signaling network Tang, Ren-Jie; Luan, Sheng Current Opinion in Plant Biology, Vol. 39 https://doi.org/10.1016/j.pbi.2017.06.009	journal	October 2017
Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications Murchie, E. H.; Lawson, T. Journal of Experimental Botany, Vol. 64, Issue 13 https://doi.org/10.1093/jxb/ert208	journal	August 2013
Calcium intolerance of fen mosses: Physiological evidence, effects of nutrient availability and successional drivers Vicherová, Eliška; Hájek, Michal; Hájek, Tomáš Perspectives in Plant Ecology, Evolution and Systematics, Vol. 17, Issue 5 https://doi.org/10.1016/j.ppees.2015.06.005	journal	October 2015
Photosynthetic performance, lipid production and biomass composition in response to nitrogen limitation in marine microalgae Jiang, Yuelu; Yoshida, Tomomi; Quigg, Antonietta Plant Physiology and Biochemistry, Vol. 54 https://doi.org/10.1016/j.plaphy.2012.02.012	journal	May 2012
Heterotrophic cultures of microalgae: Metabolism and potential products Perez-Garcia, Octavio; Escalante, Froylan M. E.; de-Bashan, Luz E. Water Research, Vol. 45, Issue 1 https://doi.org/10.1016/j.watres.2010.08.037	journal	January 2011
Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor Rodolfi, Liliana; Chini Zittelli, Graziella; Bassi, Niccolò Biotechnology and Bioengineering, Vol. 102, Issue 1 https://doi.org/10.1002/bit.22033	journal	January 2009
Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions Yao, Chang-Hong; Ai, Jiang-Ning; Cao, Xu-Peng Applied Microbiology and Biotechnology, Vol. 97, Issue 13 https://doi.org/10.1007/s00253-013-4983-x	journal	May 2013
Enhanced Accumulation of Carbohydrate and Starch in Chlorella zofingiensis Induced by Nitrogen Starvation Zhu, Shunni; Wang, Yajie; Huang, Wei Applied Biochemistry and Biotechnology, Vol. 174, Issue 7 https://doi.org/10.1007/s12010-014-1183-9	journal	September 2014
Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana Li, Tingting; Gargouri, Mahmoud; Feng, Jie Bioresource Technology, Vol. 180 https://doi.org/10.1016/j.biortech.2015.01.005	journal	March 2015
Effect of nutrient supply status on biomass composition of eukaryotic green microalgae Procházková, Gita; Brányiková, Irena; Zachleder, Vilém Journal of Applied Phycology, Vol. 26, Issue 3 https://doi.org/10.1007/s10811-013-0154-9	journal	September 2013
Cultivation of microalgae in dairy effluent for oil production and removal of organic pollution load Ummalyma, Sabeela Beevi; Sukumaran, Rajeev K. Bioresource Technology, Vol. 165 https://doi.org/10.1016/j.biortech.2014.03.028	journal	August 2014
Microalgal and cyanobacterial cultivation: The supply of nutrients Markou, Giorgos; Vandamme, Dries; Muylaert, Koenraad Water Research, Vol. 65 https://doi.org/10.1016/j.watres.2014.07.025	journal	November 2014

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