Upgrading aminoacyl-tRNA synthetases for genetic code expansion

Vargas-Rodriguez, Oscar; Sevostyanova, Anastasia; Söll, Dieter; Crnković, Ana

doi:10.1016/j.cbpa.2018.07.014

Title: Upgrading aminoacyl-tRNA synthetases for genetic code expansion

Journal Article · Fri Jul 27 00:00:00 EDT 2018 · Current Opinion in Chemical Biology

DOI:https://doi.org/10.1016/j.cbpa.2018.07.014· OSTI ID:1610394

Vargas-Rodriguez, Oscar ^[1]; Sevostyanova, Anastasia ^[1]; Söll, Dieter ^[1]; Crnković, Ana ^[1]

Yale Univ., New Haven, CT (United States)

Synthesis of proteins with non-canonical amino acids via genetic code expansion is at the forefront of synthetic biology. Progress in this field has enabled site-specific incorporation of over 200 chemically and structurally diverse amino acids into proteins in an increasing number of organisms. This has been facilitated by our ability to repurpose aminoacyl-tRNA synthetases to attach non-canonical amino acids to engineered tRNAs. Current efforts in the field focus on overcoming existing limitations to the simultaneous incorporation of multiple non-canonical amino acids or amino acids that differ from the l-α-amino acid structure (e.g. d-amino acid or β-amino acid). Here, we summarize the progress and challenges in developing more selective and efficient aminoacyl-tRNA synthetases for genetic code expansion.

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Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-98ER20311

OSTI ID:: 1610394

Alternate ID(s):: OSTI ID: 1694121

Journal Information:: Current Opinion in Chemical Biology, Vol. 46; ISSN 1367-5931

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 61 works

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References (75)

Rewriting the Genetic Code Mukai, Takahito; Lajoie, Marc J.; Englert, Markus Annual Review of Microbiology, Vol. 71, Issue 1 https://doi.org/10.1146/annurev-micro-090816-093247	journal	September 2017
Universal rules and idiosyncratic features in tRNA identity Giege, R.; Sissler, M.; Florentz, C. Nucleic Acids Research, Vol. 26, Issue 22 https://doi.org/10.1093/nar/26.22.5017	journal	November 1998
Pyrrolysyl-tRNA synthetase–tRNA^Pyl structure reveals the molecular basis of orthogonality Nozawa, Kayo; O’Donoghue, Patrick; Gundllapalli, Sarath Nature, Vol. 457, Issue 7233, p. 1163-1167 https://doi.org/10.1038/nature07611	journal	December 2008
Quality Control and Infiltration of Translation by Amino Acids Outside of the Genetic Code Bullwinkle, Tammy; Lazazzera, Beth; Ibba, Michael Annual Review of Genetics, Vol. 48, Issue 1 https://doi.org/10.1146/annurev-genet-120213-092101	journal	November 2014
Quality control despite mistranslation caused by an ambiguous genetic code Ruan, B.; Palioura, S.; Sabina, J. Proceedings of the National Academy of Sciences, Vol. 105, Issue 43 https://doi.org/10.1073/pnas.0809179105	journal	October 2008
Biosynthesis by Escherichia coli of active altered proteins containing selenium instead of sulfur Cowie, Dean B.; Cohen, Georges N. Biochimica et Biophysica Acta, Vol. 26, Issue 2 https://doi.org/10.1016/0006-3002(57)90003-3	journal	November 1957
Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three-dimensional structure. Hendrickson, W. A.; Horton, J. R.; LeMaster, D. M. The EMBO Journal, Vol. 9, Issue 5 https://doi.org/10.1002/j.1460-2075.1990.tb08287.x	journal	May 1990
In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons Dieterich, Daniela C.; Hodas, Jennifer J. L.; Gouzer, Géraldine Nature Neuroscience, Vol. 13, Issue 7 https://doi.org/10.1038/nn.2580	journal	June 2010
Expanding the genetic code of Salmonella with non-canonical amino acids Gan, Qinglei; Lehman, Brent P.; Bobik, Thomas A. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep39920	journal	December 2016
Development of an Unnatural Amino Acid Incorporation System in the Actinobacterial Natural Product Producer Streptomyces venezuelae ATCC 15439 He, Jingxuan; Van Treeck, Briana; Nguyen, Han B. ACS Synthetic Biology, Vol. 5, Issue 2 https://doi.org/10.1021/acssynbio.5b00209	journal	November 2015
An Expanded Eukaryotic Genetic Code Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher Science, Vol. 301, Issue 5635, p. 964-967 https://doi.org/10.1126/science.1084772	journal	August 2003
Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase Suzuki, Tateki; Miller, Corwin; Guo, Li-Tao Nature Chemical Biology, Vol. 13, Issue 12 https://doi.org/10.1038/nchembio.2497	journal	October 2017
Expanded Genetic Code Technologies for Incorporating Modified Lysine at Multiple Sites Yanagisawa, Tatsuo; Umehara, Takashi; Sakamoto, Kensaku ChemBioChem, Vol. 15, Issue 15 https://doi.org/10.1002/cbic.201402266	journal	September 2014
De Novo Enzyme Design Using Rosetta3 Richter, Florian; Leaver-Fay, Andrew; Khare, Sagar D. PLoS ONE, Vol. 6, Issue 5 https://doi.org/10.1371/journal.pone.0019230	journal	May 2011
Directed Evolution of Protein Catalysts Zeymer, Cathleen; Hilvert, Donald Annual Review of Biochemistry, Vol. 87, Issue 1 https://doi.org/10.1146/annurev-biochem-062917-012034	journal	June 2018
Progress toward the evolution of an organism with an expanded genetic code Liu, David R.; Schultz, Peter G. Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785 https://doi.org/10.1073/pnas.96.9.4780	journal	April 1999
Genomes by design Haimovich, Adrian D.; Muir, Paul; Isaacs, Farren J. Nature Reviews Genetics, Vol. 16, Issue 9 https://doi.org/10.1038/nrg3956	journal	August 2015
Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids Amiram, Miriam; Haimovich, Adrian D.; Fan, Chenguang Nature Biotechnology, Vol. 33, Issue 12 https://doi.org/10.1038/nbt.3372	journal	November 2015
Methods for the directed evolution of proteins Packer, Michael S.; Liu, David R. Nature Reviews Genetics, Vol. 16, Issue 7 https://doi.org/10.1038/nrg3927	journal	June 2015
Development of potent in vivo mutagenesis plasmids with broad mutational spectra Badran, Ahmed H.; Liu, David R. Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms9425	journal	October 2015
Continuous directed evolution of aminoacyl-tRNA synthetases Bryson, David I.; Fan, Chenguang; Guo, Li-Tao Nature Chemical Biology, Vol. 13, Issue 12 https://doi.org/10.1038/nchembio.2474	journal	October 2017
Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code Hauf, Matthias; Richter, Florian; Schneider, Tobias ChemBioChem, Vol. 18, Issue 18 https://doi.org/10.1002/cbic.201700327	journal	August 2017
Evolving tRNA^Sec for Efficient Canonical Incorporation of Selenocysteine Thyer, Ross; Robotham, Scott A.; Brodbelt, Jennifer S. Journal of the American Chemical Society, Vol. 137, Issue 1, p. 46-49 https://doi.org/10.1021/ja510695g	journal	December 2014
Expanding the Genetic Code of Escherichia coli with Phosphoserine Park, H.-S.; Hohn, M. J.; Umehara, T. Science, Vol. 333, Issue 6046, p. 1151-1154 https://doi.org/10.1126/science.1207203	journal	August 2011
Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog Rogerson, Daniel T.; Sachdeva, Amit; Wang, Kaihang Nature Chemical Biology, Vol. 11, Issue 7 https://doi.org/10.1038/nchembio.1823	journal	June 2015
Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET Wang, Kaihang; Sachdeva, Amit; Cox, Daniel J. Nature Chemistry, Vol. 6, Issue 5 https://doi.org/10.1038/nchem.1919	journal	April 2014
Exploring the Substrate Range of Wild-Type Aminoacyl-tRNA Synthetases Fan, Chenguang; Ho, Joanne M. L.; Chirathivat, Napon ChemBioChem, Vol. 15, Issue 12 https://doi.org/10.1002/cbic.201402083	journal	May 2014
Engineering the elongation factor Tu for efficient selenoprotein synthesis Haruna, Ken-ichi; Alkazemi, Muhammad H.; Liu, Yuchen Nucleic Acids Research, Vol. 42, Issue 15, p. 9976-9983 https://doi.org/10.1093/nar/gku691	journal	July 2014
Directed Evolution of Heterologous tRNAs Leads to Reduced Dependence on Post-transcriptional Modifications Baldridge, Kevin C.; Jora, Manasses; Maranhao, Andre C. ACS Synthetic Biology, Vol. 7, Issue 5 https://doi.org/10.1021/acssynbio.7b00421	journal	April 2018
Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing Zhang, Michael Shaofei; Brunner, Simon F.; Huguenin-Dezot, Nicolas Nature Methods, Vol. 14, Issue 7 https://doi.org/10.1038/nmeth.4302	journal	May 2017
Genetic Incorporation of Twelve meta -Substituted Phenylalanine Derivatives Using a Single Pyrrolysyl-tRNA Synthetase Mutant Wang, Yane-Shih; Fang, Xinqiang; Chen, Hsueh-Ying ACS Chemical Biology, Vol. 8, Issue 2 https://doi.org/10.1021/cb300512r	journal	November 2012
Site-Specific Bioorthogonal Labeling for Fluorescence Imaging of Intracellular Proteins in Living Cells Peng, Tao; Hang, Howard C. Journal of the American Chemical Society, Vol. 138, Issue 43 https://doi.org/10.1021/jacs.6b08733	journal	October 2016
Thiazolidine-Masked α-Oxo Aldehyde Functionality for Peptide and Protein Modification Bi, Xiaobao; Pasunooti, Kalyan Kumar; Lescar, Julien Bioconjugate Chemistry, Vol. 28, Issue 2 https://doi.org/10.1021/acs.bioconjchem.6b00667	journal	December 2016
A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein-Protein Interactions Joiner, Cassandra M.; Breen, Meghan E.; Clayton, James ChemBioChem, Vol. 18, Issue 2 https://doi.org/10.1002/cbic.201600578	journal	December 2016
Engineering posttranslational proofreading to discriminate nonstandard amino acids Kunjapur, Aditya M.; Stork, Devon A.; Kuru, Erkin Proceedings of the National Academy of Sciences, Vol. 115, Issue 3 https://doi.org/10.1073/pnas.1715137115	journal	January 2018
Ribosomal Synthesis of Peptides with Multiple β-Amino Acids Fujino, Tomoshige; Goto, Yuki; Suga, Hiroaki Journal of the American Chemical Society, Vol. 138, Issue 6 https://doi.org/10.1021/jacs.5b12482	journal	February 2016
Consecutive Elongation of D-Amino Acids in Translation Katoh, Takayuki; Tajima, Kenya; Suga, Hiroaki Cell Chemical Biology, Vol. 24, Issue 1 https://doi.org/10.1016/j.chembiol.2016.11.012	journal	January 2017
A highly flexible tRNA acylation method for non-natural polypeptide synthesis Murakami, Hiroshi; Ohta, Atsushi; Ashigai, Hiroshi Nature Methods, Vol. 3, Issue 5 https://doi.org/10.1038/nmeth877	journal	April 2006
Enzymatic aminoacylation of tRNA with unnatural amino acids Hartman, M. C. T.; Josephson, K.; Szostak, J. W. Proceedings of the National Academy of Sciences, Vol. 103, Issue 12 https://doi.org/10.1073/pnas.0509219103	journal	March 2006
Recognition of Non-α-amino Substrates by Pyrrolysyl-tRNA Synthetase Kobayashi, Takatsugu; Yanagisawa, Tatsuo; Sakamoto, Kensaku Journal of Molecular Biology, Vol. 385, Issue 5 https://doi.org/10.1016/j.jmb.2008.11.059	journal	February 2009
In Vivo Biosynthesis of a β-Amino Acid-Containing Protein Melo Czekster, Clarissa; Robertson, Wesley E.; Walker, Allison S. Journal of the American Chemical Society, Vol. 138, Issue 16 https://doi.org/10.1021/jacs.6b01023	journal	January 2016
Incorporation of β-amino acids into dihydrofolate reductase by ribosomes having modifications in the peptidyltransferase center Maini, Rumit; Nguyen, Dan T.; Chen, Shengxi Bioorganic & Medicinal Chemistry, Vol. 21, Issue 5 https://doi.org/10.1016/j.bmc.2013.01.002	journal	March 2013
An expanded genetic code with a functional quadruplet codon Anderson, J. C.; Wu, N.; Santoro, S. W. Proceedings of the National Academy of Sciences, Vol. 101, Issue 20, p. 7566-7571 https://doi.org/10.1073/pnas.0401517101	journal	May 2004
Expanding the Scope of Single- and Double-Noncanonical Amino Acid Mutagenesis in Mammalian Cells Using Orthogonal Polyspecific Leucyl-tRNA Synthetases Zheng, Yunan; Mukherjee, Raja; Chin, Melissa A. Biochemistry, Vol. 57, Issue 4 https://doi.org/10.1021/acs.biochem.7b00952	journal	November 2017
Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon Mukai, Takahito; Hoshi, Hiroko; Ohtake, Kazumasa Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep09699	journal	May 2015
Genomically Recoded Organisms Expand Biological Functions Lajoie, M. J.; Rovner, A. J.; Goodman, D. B. Science, Vol. 342, Issue 6156, p. 357-360 https://doi.org/10.1126/science.1241459	journal	October 2013
Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli Mukai, Takahito; Yamaguchi, Atsushi; Ohtake, Kazumasa Nucleic Acids Research, Vol. 43, Issue 16 https://doi.org/10.1093/nar/gkv787	journal	August 2015
Design of a synthetic yeast genome Richardson, Sarah M.; Mitchell, Leslie A.; Stracquadanio, Giovanni Science, Vol. 355, Issue 6329 https://doi.org/10.1126/science.aaf4557	journal	March 2017
Frameshift Mutations Roth, J. R. Annual Review of Genetics, Vol. 8, Issue 1 https://doi.org/10.1146/annurev.ge.08.120174.001535	journal	December 1974
Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome Neumann, Heinz; Wang, Kaihang; Davis, Lloyd Nature, Vol. 464, Issue 7287 https://doi.org/10.1038/nature08817	journal	February 2010
Defining synonymous codon compression schemes by genome recoding Wang, Kaihang; Fredens, Julius; Brunner, Simon F. Nature, Vol. 539, Issue 7627 https://doi.org/10.1038/nature20124	journal	October 2016
Design, synthesis, and testing toward a 57-codon genome Ostrov, N.; Landon, M.; Guell, M. Science, Vol. 353, Issue 6301 https://doi.org/10.1126/science.aaf3639	journal	August 2016
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering Garst, Andrew D.; Bassalo, Marcelo C.; Pines, Gur Nature Biotechnology, Vol. 35, Issue 1, p. 48-55 https://doi.org/10.1038/nbt.3718	journal	December 2016
Roles for Synonymous Codon Usage in Protein Biogenesis Chaney, Julie L.; Clark, Patricia L. Annual Review of Biophysics, Vol. 44, Issue 1 https://doi.org/10.1146/annurev-biophys-060414-034333	journal	June 2015
A semi-synthetic organism with an expanded genetic alphabet Malyshev, Denis A.; Dhami, Kirandeep; Lavergne, Thomas Nature, Vol. 509, Issue 7500, p. 385-388 https://doi.org/10.1038/nature13314	journal	May 2014
A semi-synthetic organism that stores and retrieves increased genetic information Zhang, Yorke; Ptacin, Jerod L.; Fischer, Emil C. Nature, Vol. 551, Issue 7682 https://doi.org/10.1038/nature24659	journal	November 2017
Phosphoserine aminoacylation of tRNA bearing an unnatural base anticodon Fukunaga, Ryuya; Harada, Yoko; Hirao, Ichiro Biochemical and Biophysical Research Communications, Vol. 372, Issue 3 https://doi.org/10.1016/j.bbrc.2008.05.078	journal	August 2008
Functional Replacement of Histidine in Proteins To Generate Noncanonical Amino Acid Dependent Organisms Gan, Fei; Liu, Renhe; Wang, Feng Journal of the American Chemical Society, Vol. 140, Issue 11 https://doi.org/10.1021/jacs.7b13452	journal	March 2018
Generation of influenza A viruses as live but replication-incompetent virus vaccines Si, L.; Xu, H.; Zhou, X. Science, Vol. 354, Issue 6316 https://doi.org/10.1126/science.aah5869	journal	December 2016
Evolution of a designed protein assembly encapsulating its own RNA genome Butterfield, Gabriel L.; Lajoie, Marc J.; Gustafson, Heather H. Nature, Vol. 552, Issue 7685 https://doi.org/10.1038/nature25157	journal	December 2017
Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria Luo, Xiaozhou; Fu, Guangsen; Wang, Rongsheng E. Nature Chemical Biology, Vol. 13, Issue 8 https://doi.org/10.1038/nchembio.2405	journal	June 2017
Fluorothreonyl-tRNA deacylase prevents mistranslation in the organofluorine producer Streptomyces cattleya McMurry, Jonathan L.; Chang, Michelle C. Y. Proceedings of the National Academy of Sciences, Vol. 114, Issue 45 https://doi.org/10.1073/pnas.1711482114	journal	October 2017
Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function Ko, Jae-hyeong; Wang, Yane-Shih; Nakamura, Akiyoshi FEBS Letters, Vol. 587, Issue 19 https://doi.org/10.1016/j.febslet.2013.08.018	journal	August 2013
Expanding the Genetic Code of Caenorhabditis elegans Using Bacterial Aminoacyl-tRNA Synthetase/tRNA Pairs Parrish, Angela R.; She, Xingyu; Xiang, Zheng ACS Chemical Biology, Vol. 7, Issue 7 https://doi.org/10.1021/cb200542j	journal	May 2012
Heritable expansion of the genetic code in mouse and zebrafish Chen, Yuting; Ma, Ji; Lu, Wenqing Cell Research, Vol. 27, Issue 2 https://doi.org/10.1038/cr.2016.145	journal	December 2016
Expanding the genetic code of Mus musculus Han, Songmi; Yang, Aerin; Lee, Soonjang Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14568	journal	February 2017
Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases Mukai, Takahito; Kobayashi, Takatsugu; Hino, Nobumasa Biochemical and Biophysical Research Communications, Vol. 371, Issue 4 https://doi.org/10.1016/j.bbrc.2008.04.164	journal	July 2008
Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair Hancock, Susan M.; Uprety, Rajendra; Deiters, Alexander Journal of the American Chemical Society, Vol. 132, Issue 42 https://doi.org/10.1021/ja104609m	journal	October 2010
Expanding the Genetic Code for Photoclick Chemistry in E. coli , Mammalian Cells, and A. thaliana Li, Fahui; Zhang, Hua; Sun, Yun Angewandte Chemie International Edition, Vol. 52, Issue 37 https://doi.org/10.1002/anie.201303477	journal	July 2013
Genetically encoding unnatural amino acids for cellular and neuronal studies Wang, Wenyuan; Takimoto, Jeffrey K.; Louie, Gordon V. Nature Neuroscience, Vol. 10, Issue 8 https://doi.org/10.1038/nn1932	journal	July 2007
New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast Wang, Qian; Wang, Lei Journal of the American Chemical Society, Vol. 130, Issue 19, p. 6066-6067 https://doi.org/10.1021/ja800894n	journal	May 2008
An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes Italia, James S.; Addy, Partha Sarathi; Wrobel, Chester J. J. Nature Chemical Biology, Vol. 13, Issue 4 https://doi.org/10.1038/nchembio.2312	journal	February 2017
A Tryptophanyl-tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis in E. coli Chatterjee, Abhishek; Xiao, Han; Yang, Peng-Yu Angewandte Chemie International Edition, Vol. 52, Issue 19 https://doi.org/10.1002/anie.201301094	journal	April 2013
Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA Hughes, Randall A.; Ellington, Andrew D. Nucleic Acids Research, Vol. 38, Issue 19 https://doi.org/10.1093/nar/gkq521	journal	June 2010
Breaking the Degeneracy of the Genetic Code Kwon, Inchan; Kirshenbaum, Kent; Tirrell, David A. Journal of the American Chemical Society, Vol. 125, Issue 25 https://doi.org/10.1021/ja0350076	journal	June 2003

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