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Title: Optical coherence tomography imaging of plant root growth in soil

Abstract

Complex interactions between roots and soil provide the nutrients and physical support required for robust plant growth. Yet, visualizing the root-soil interface is challenged by soil’s opaque scattering characteristics. Herein, we describe methods for using optical coherence tomography (OCT) to provide non-destructive 3D and cross-sectional root imaging not available with traditional bright field microscopy. OCT is regularly used for bioimaging, especially in ophthalmology where it can detect retinal abnormalities. Prior use of OCT in plant biology has focused on surface defects of above ground tissues, predominantly in food crops. Our results show OCT is also viable for detailed, in situ study of living plant roots.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. BATTELLE (PACIFIC NW LAB)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1605095
Report Number(s):
PNNL-SA-149582
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Applied Optics
Additional Journal Information:
Journal Volume: 59; Journal Issue: 8
Country of Publication:
United States
Language:
English
Subject:
optical coherence tomography, switchgrass, imaging plant roots

Citation Formats

Larimer, Curtis J., Denis, Elizabeth H., Suter, Jonathan D., and Moran, James J. Optical coherence tomography imaging of plant root growth in soil. United States: N. p., 2020. Web. doi:10.1364/AO.384674.
Larimer, Curtis J., Denis, Elizabeth H., Suter, Jonathan D., & Moran, James J. Optical coherence tomography imaging of plant root growth in soil. United States. doi:10.1364/AO.384674.
Larimer, Curtis J., Denis, Elizabeth H., Suter, Jonathan D., and Moran, James J. Tue . "Optical coherence tomography imaging of plant root growth in soil". United States. doi:10.1364/AO.384674.
@article{osti_1605095,
title = {Optical coherence tomography imaging of plant root growth in soil},
author = {Larimer, Curtis J. and Denis, Elizabeth H. and Suter, Jonathan D. and Moran, James J.},
abstractNote = {Complex interactions between roots and soil provide the nutrients and physical support required for robust plant growth. Yet, visualizing the root-soil interface is challenged by soil’s opaque scattering characteristics. Herein, we describe methods for using optical coherence tomography (OCT) to provide non-destructive 3D and cross-sectional root imaging not available with traditional bright field microscopy. OCT is regularly used for bioimaging, especially in ophthalmology where it can detect retinal abnormalities. Prior use of OCT in plant biology has focused on surface defects of above ground tissues, predominantly in food crops. Our results show OCT is also viable for detailed, in situ study of living plant roots.},
doi = {10.1364/AO.384674},
journal = {Applied Optics},
number = 8,
volume = 59,
place = {United States},
year = {2020},
month = {3}
}

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