skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nanobody-enabled monitoring of kappa opioid receptor states

Authors:
ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo; ; ORCiD logo; ; ; ; ORCiD logo; ORCiD logo; ; ORCiD logo
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1604214
Resource Type:
Journal Article
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Che, Tao, English, Justin, Krumm, Brian E., Kim, Kuglae, Pardon, Els, Olsen, Reid H. J., Wang, Sheng, Zhang, Shicheng, Diberto, Jeffrey F., Sciaky, Noah, Carroll, F. Ivy, Steyaert, Jan, Wacker, Daniel, and Roth, Bryan L. Nanobody-enabled monitoring of kappa opioid receptor states. United States: N. p., 2020. Web. doi:10.1038/s41467-020-14889-7.
Che, Tao, English, Justin, Krumm, Brian E., Kim, Kuglae, Pardon, Els, Olsen, Reid H. J., Wang, Sheng, Zhang, Shicheng, Diberto, Jeffrey F., Sciaky, Noah, Carroll, F. Ivy, Steyaert, Jan, Wacker, Daniel, & Roth, Bryan L. Nanobody-enabled monitoring of kappa opioid receptor states. United States. doi:10.1038/s41467-020-14889-7.
Che, Tao, English, Justin, Krumm, Brian E., Kim, Kuglae, Pardon, Els, Olsen, Reid H. J., Wang, Sheng, Zhang, Shicheng, Diberto, Jeffrey F., Sciaky, Noah, Carroll, F. Ivy, Steyaert, Jan, Wacker, Daniel, and Roth, Bryan L. Mon . "Nanobody-enabled monitoring of kappa opioid receptor states". United States. doi:10.1038/s41467-020-14889-7.
@article{osti_1604214,
title = {Nanobody-enabled monitoring of kappa opioid receptor states},
author = {Che, Tao and English, Justin and Krumm, Brian E. and Kim, Kuglae and Pardon, Els and Olsen, Reid H. J. and Wang, Sheng and Zhang, Shicheng and Diberto, Jeffrey F. and Sciaky, Noah and Carroll, F. Ivy and Steyaert, Jan and Wacker, Daniel and Roth, Bryan L.},
abstractNote = {},
doi = {10.1038/s41467-020-14889-7},
journal = {Nature Communications},
issn = {2041-1723},
number = 1,
volume = 11,
place = {United States},
year = {2020},
month = {3}
}

Works referenced in this record:

Crystal Structure of an LSD-Bound Human Serotonin Receptor
journal, January 2017


Position of Transmembrane Helix 6 Determines Receptor G Protein Coupling Specificity
journal, July 2014

  • Rose, Alexander S.; Elgeti, Matthias; Zachariae, Ulrich
  • Journal of the American Chemical Society, Vol. 136, Issue 32
  • DOI: 10.1021/ja5055109

Mini G protein probes for active G protein–coupled receptors (GPCRs) in live cells
journal, March 2018

  • Wan, Qingwen; Okashah, Najeah; Inoue, Asuka
  • Journal of Biological Chemistry, Vol. 293, Issue 19
  • DOI: 10.1074/jbc.RA118.001975

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

  • Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
  • DOI: 10.1107/S0907444996012255

How Ligands Illuminate GPCR Molecular Pharmacology
journal, July 2017


Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser
journal, July 2015

  • Kang, Yanyong; Zhou, X. Edward; Gao, Xiang
  • Nature, Vol. 523, Issue 7562
  • DOI: 10.1038/nature14656

Transduction of Receptor Signals by  -Arrestins
journal, April 2005


NIDA’s medication development priorities in response to the Opioid Crisis: ten most wanted
journal, December 2018


PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome
journal, April 2015

  • Kroeze, Wesley K.; Sassano, Maria F.; Huang, Xi-Ping
  • Nature Structural & Molecular Biology, Vol. 22, Issue 5
  • DOI: 10.1038/nsmb.3014

Conformational biosensors reveal GPCR signalling from endosomes
journal, March 2013

  • Irannejad, Roshanak; Tomshine, Jin C.; Tomshine, Jon R.
  • Nature, Vol. 495, Issue 7442
  • DOI: 10.1038/nature12000

Structure of the human κ-opioid receptor in complex with JDTic
journal, March 2012

  • Wu, Huixian; Wacker, Daniel; Mileni, Mauro
  • Nature, Vol. 485, Issue 7398
  • DOI: 10.1038/nature10939

Structure of the µ-opioid receptor–Gi protein complex
journal, June 2018


Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody
journal, January 2019


Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling
journal, May 2015


Regulation of β 2 -Adrenergic Receptor Function by Conformationally Selective Single-Domain Intrabodies
journal, December 2013

  • Staus, Dean P.; Wingler, Laura M.; Strachan, Ryan T.
  • Molecular Pharmacology, Vol. 85, Issue 3
  • DOI: 10.1124/mol.113.089516

A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action
journal, June 2018


Nanobody-Enabled Reverse Pharmacology on G-Protein-Coupled Receptors
journal, March 2018

  • Pardon, Els; Betti, Cecilia; Laeremans, Toon
  • Angewandte Chemie International Edition, Vol. 57, Issue 19
  • DOI: 10.1002/anie.201712581

The Dynamic Process of β2-Adrenergic Receptor Activation
journal, January 2013


Antidepressant-Like Effects of the Novel Kappa Opioid Antagonist MCL-144B in the Forced-Swim Test
journal, January 2008

  • Reindl, J. D.; Rowan, K.; Carey, A. N.
  • Pharmacology, Vol. 81, Issue 3
  • DOI: 10.1159/000112867

Crystallizing membrane proteins using lipidic mesophases
journal, April 2009


Identification of two distinct inactive conformations of the  2-adrenergic receptor reconciles structural and biochemical observations
journal, March 2009

  • Dror, R. O.; Arlow, D. H.; Borhani, D. W.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 12
  • DOI: 10.1073/pnas.0811065106

Structure of the D2 dopamine receptor bound to the atypical antipsychotic drug risperidone
journal, January 2018


Serial Femtosecond Crystallography of G Protein-Coupled Receptors
journal, December 2013


Salvinorin A: A potent naturally occurring nonnitrogenous   opioid selective agonist
journal, August 2002

  • Roth, B. L.; Baner, K.; Westkaemper, R.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 18
  • DOI: 10.1073/pnas.182234399

Structure of a nanobody-stabilized active state of the β2 adrenoceptor
journal, January 2011

  • Rasmussen, Søren G. F.; Choi, Hee-Jung; Fung, Juan Jose
  • Nature, Vol. 469, Issue 7329
  • DOI: 10.1038/nature09648

Structure of the Nanobody-Stabilized Active State of the Kappa Opioid Receptor
journal, January 2018


Redesign of a Four-helix Bundle Protein by Phage Display Coupled with Proteolysis and Structural Characterization by NMR and X-ray Crystallography
journal, October 2002


Activation and allosteric modulation of a muscarinic acetylcholine receptor
journal, November 2013

  • Kruse, Andrew C.; Ring, Aaron M.; Manglik, Aashish
  • Nature, Vol. 504, Issue 7478
  • DOI: 10.1038/nature12735

Identification of Novel Functionally Selective κ -Opioid Receptor Scaffolds
journal, October 2013

  • White, Kate L.; Scopton, Alex P.; Rives, Marie-Laure
  • Molecular Pharmacology, Vol. 85, Issue 1
  • DOI: 10.1124/mol.113.089649

Yeast surface display platform for rapid discovery of conformationally selective nanobodies
journal, February 2018

  • McMahon, Conor; Baier, Alexander S.; Pascolutti, Roberta
  • Nature Structural & Molecular Biology, Vol. 25, Issue 3
  • DOI: 10.1038/s41594-018-0028-6

Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria
journal, November 2016


G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody
journal, January 2012

  • Hino, Tomoya; Arakawa, Takatoshi; Iwanari, Hiroko
  • Nature, Vol. 482, Issue 7384
  • DOI: 10.1038/nature10750

HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes
journal, July 2006

  • Minor, Wladek; Cymborowski, Marcin; Otwinowski, Zbyszek
  • Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8
  • DOI: 10.1107/S0907444906019949

Allosteric coupling from G protein to the agonist-binding pocket in GPCRs
journal, June 2016

  • DeVree, Brian T.; Mahoney, Jacob P.; Vélez-Ruiz, Gisselle A.
  • Nature, Vol. 535, Issue 7610
  • DOI: 10.1038/nature18324

Probe Dependence in the Allosteric Modulation of a G Protein-Coupled Receptor: Implications for Detection and Validation of Allosteric Ligand Effects
journal, October 2011

  • Valant, Celine; Felder, Christian C.; Sexton, Patrick M.
  • Molecular Pharmacology, Vol. 81, Issue 1
  • DOI: 10.1124/mol.111.074872

Crystal structure of the β2 adrenergic receptor–Gs protein complex
journal, July 2011

  • Rasmussen, Søren G. F.; DeVree, Brian T.; Zou, Yaozhong
  • Nature, Vol. 477, Issue 7366
  • DOI: 10.1038/nature10361

The G Protein–Biased κ -Opioid Receptor Agonist RB-64 Is Analgesic with a Unique Spectrum of Activities In Vivo
journal, October 2014

  • White, Kate L.; Robinson, J. Elliott; Zhu, Hu
  • Journal of Pharmacology and Experimental Therapeutics, Vol. 352, Issue 1
  • DOI: 10.1124/jpet.114.216820

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation
journal, July 2016

  • Staus, Dean P.; Strachan, Ryan T.; Manglik, Aashish
  • Nature, Vol. 535, Issue 7612
  • DOI: 10.1038/nature18636

A general protocol for the generation of Nanobodies for structural biology
journal, February 2014


Structure-based discovery of selective positive allosteric modulators of antagonists for the M 2 muscarinic acetylcholine receptor
journal, February 2018

  • Korczynska, Magdalena; Clark, Mary J.; Valant, Celine
  • Proceedings of the National Academy of Sciences, Vol. 115, Issue 10
  • DOI: 10.1073/pnas.1718037115

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor
journal, June 2018

  • Livingston, Kathryn E.; Mahoney, Jacob P.; Manglik, Aashish
  • eLife, Vol. 7
  • DOI: 10.7554/eLife.32499

Fiji: an open-source platform for biological-image analysis
journal, June 2012

  • Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2019

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

A conserved molecular switch in Class F receptors regulates receptor activation and pathway selection
journal, February 2019

  • Wright, Shane C.; Kozielewicz, Paweł; Kowalski-Jahn, Maria
  • Nature Communications, Vol. 10, Issue 1
  • DOI: 10.1038/s41467-019-08630-2

Identification of the First trans -(3 R ,4 R )- Dimethyl-4-(3-hydroxyphenyl)piperidine Derivative To Possess Highly Potent and Selective Opioid κ Receptor Antagonist Activity
journal, August 2001

  • Thomas, James B.; Atkinson, Robert N.; Rothman, Richard B.
  • Journal of Medicinal Chemistry, Vol. 44, Issue 17
  • DOI: 10.1021/jm015521r

Discovery of new GPCR ligands to illuminate new biology
journal, October 2017

  • Roth, Bryan L.; Irwin, John J.; Shoichet, Brian K.
  • Nature Chemical Biology, Vol. 13, Issue 11
  • DOI: 10.1038/nchembio.2490

Molecular control of δ-opioid receptor signalling
journal, January 2014

  • Fenalti, Gustavo; Giguere, Patrick M.; Katritch, Vsevolod
  • Nature, Vol. 506, Issue 7487
  • DOI: 10.1038/nature12944

G protein signaling–biased agonism at the κ-opioid receptor is maintained in striatal neurons
journal, August 2018


Structural insights into µ-opioid receptor activation
journal, August 2015

  • Huang, Weijiao; Manglik, Aashish; Venkatakrishnan, A. J.
  • Nature, Vol. 524, Issue 7565
  • DOI: 10.1038/nature14886

Functional Selectivity and Classical Concepts of Quantitative Pharmacology
journal, June 2006

  • Urban, Jonathan D.; Clarke, William P.; von Zastrow, Mark
  • Journal of Pharmacology and Experimental Therapeutics, Vol. 320, Issue 1
  • DOI: 10.1124/jpet.106.104463

Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206