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Title: Active site flexibility revealed in crystal structures of Parabacteroides merdae β-glucuronidase from the human gut microbiome: Crystal Structure of P. merdae β-Glucuronidase

Authors:
 [1];  [1];  [1];  [2];  [3];  [3];  [4]
  1. Department of Chemistry, University of North Carolina, Chapel Hill North Carolina 27599-3290
  2. Department of Biochemistry & Biophysics, University of North Carolina, Chapel Hill North Carolina 27599-3290
  3. Department of Chemical Biology and Medicinal Chemistry, University of North Carolina, Chapel Hill North Carolina 27599-3290
  4. Department of Chemistry, University of North Carolina, Chapel Hill North Carolina 27599-3290; Department of Biochemistry & Biophysics, University of North Carolina, Chapel Hill North Carolina 27599-3290; Department of Microbiology & Immunology, University of North Carolina, Chapel Hill North Carolina 27599-3290; The Integrated Program for Biological and Genome Sciences, University of North Carolina, Chapel Hill North Carolina 27599-3290
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1604174
Resource Type:
Journal Article
Journal Name:
Protein Science
Additional Journal Information:
Journal Volume: 27; Journal Issue: 12; Journal ID: ISSN 0961-8368
Publisher:
The Protein Society
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Little, Michael S., Ervin, Samantha M., Walton, William G., Tripathy, Ashutosh, Xu, Yongmei, Liu, Jian, and Redinbo, Matthew R. Active site flexibility revealed in crystal structures of Parabacteroides merdae β-glucuronidase from the human gut microbiome: Crystal Structure of P. merdae β-Glucuronidase. United States: N. p., 2018. Web. doi:10.1002/PRO.3507.
Little, Michael S., Ervin, Samantha M., Walton, William G., Tripathy, Ashutosh, Xu, Yongmei, Liu, Jian, & Redinbo, Matthew R. Active site flexibility revealed in crystal structures of Parabacteroides merdae β-glucuronidase from the human gut microbiome: Crystal Structure of P. merdae β-Glucuronidase. United States. doi:10.1002/PRO.3507.
Little, Michael S., Ervin, Samantha M., Walton, William G., Tripathy, Ashutosh, Xu, Yongmei, Liu, Jian, and Redinbo, Matthew R. Sat . "Active site flexibility revealed in crystal structures of Parabacteroides merdae β-glucuronidase from the human gut microbiome: Crystal Structure of P. merdae β-Glucuronidase". United States. doi:10.1002/PRO.3507.
@article{osti_1604174,
title = {Active site flexibility revealed in crystal structures of Parabacteroides merdae β-glucuronidase from the human gut microbiome: Crystal Structure of P. merdae β-Glucuronidase},
author = {Little, Michael S. and Ervin, Samantha M. and Walton, William G. and Tripathy, Ashutosh and Xu, Yongmei and Liu, Jian and Redinbo, Matthew R.},
abstractNote = {},
doi = {10.1002/PRO.3507},
journal = {Protein Science},
issn = {0961-8368},
number = 12,
volume = 27,
place = {United States},
year = {2018},
month = {10}
}

Works referenced in this record:

The impact of glucuronidation on the bioactivation and DNA adduction of the cooked-food carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine in vivo
journal, June 2005

  • Malfatti, Michael A.; Ubick, Esther A.; Felton, James S.
  • Carcinogenesis, Vol. 26, Issue 11
  • DOI: 10.1093/carcin/bgi151

Alginate: Properties and biomedical applications
journal, January 2012


Modification of Lactobacillus β-glucuronidase activity by random mutagenesis
journal, March 2007


How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans
journal, June 2017

  • Cartmell, Alan; Lowe, Elisabeth C.; Baslé, Arnaud
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 27
  • DOI: 10.1073/pnas.1704367114

MUC1 cell surface mucin is a critical element of the mucosal barrier to infection
journal, August 2007

  • McAuley, Julie L.; Linden, Sara K.; Png, Chin Wen
  • Journal of Clinical Investigation, Vol. 117, Issue 8
  • DOI: 10.1172/JCI26705

Dali server update
journal, April 2016

  • Holm, Liisa; Laakso, Laura M.
  • Nucleic Acids Research, Vol. 44, Issue W1
  • DOI: 10.1093/nar/gkw357

Glucuronide formation in the transport of testosterone and androstenedione by rat intestine
journal, January 1963


The gastrointestinal microbiota as a site for the biotransformation of drugs
journal, November 2008


The human microbiome is a source of therapeutic drug targets
journal, June 2013


Protein production by auto-induction in high-density shaking cultures
journal, May 2005


An Atlas of β-Glucuronidases in the Human Intestinal Microbiome
journal, July 2017


Alterations of plasma glycosaminoglycan profile in patients with rheumatoid arthritis in relation to disease activity
journal, June 2014

  • Jura-Półtorak, Agnieszka; Komosinska-Vassev, Katarzyna; Kotulska, Anna
  • Clinica Chimica Acta, Vol. 433
  • DOI: 10.1016/j.cca.2014.02.027

Pharmacologic Targeting of Bacterial β-Glucuronidase Alleviates Nonsteroidal Anti-Inflammatory Drug-Induced Enteropathy in Mice
journal, February 2012

  • LoGuidice, Amanda; Wallace, Bret D.; Bendel, Lauren
  • Journal of Pharmacology and Experimental Therapeutics, Vol. 341, Issue 2
  • DOI: 10.1124/jpet.111.191122

Chondroitin - application in the treatment of degenerative joint disease
journal, November 2016


Atomic Resolution Cryo-EM Structure of β-Galactosidase
journal, June 2018


Microbial Glucuronidase Inhibition Reduces Severity of Diclofenac-Induced Anastomotic Leak in Rats
journal, May 2018

  • Yauw, Simon T. K.; Arron, Melissa; Lomme, Roger M. L. M.
  • Surgical Infections, Vol. 19, Issue 4
  • DOI: 10.1089/sur.2017.245

Drug glucuronidation in humans
journal, January 1991


Glycan complexity dictates microbial resource allocation in the large intestine
journal, June 2015

  • Rogowski, Artur; Briggs, Jonathon A.; Mortimer, Jennifer C.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms8481

Chemical transformation of xenobiotics by the human gut microbiota
journal, June 2017


Understanding and Modulating Mammalian-Microbial Communication for Improved Human Health
journal, January 2014


Molecular Insights into Microbial β -Glucuronidase Inhibition to Abrogate CPT-11 Toxicity
journal, May 2013

  • Roberts, Adam B.; Wallace, Bret D.; Venkatesh, Madhu Kumar
  • Molecular Pharmacology, Vol. 84, Issue 2
  • DOI: 10.1124/mol.113.085852

How glycan metabolism shapes the human gut microbiota
journal, April 2012

  • Koropatkin, Nicole M.; Cameron, Elizabeth A.; Martens, Eric C.
  • Nature Reviews Microbiology, Vol. 10, Issue 5
  • DOI: 10.1038/nrmicro2746

On serum-cholesterol levels and neutral fecal sterols in germ-free rats. Bile acids and steroids 59
journal, August 1959


Human UDP-Glucuronosyltransferase 1A1 Is the Primary Enzyme Responsible for the N -Glucuronidation of N -Hydroxy-PhIP in Vitro
journal, August 2004

  • Malfatti, Michael A.; Felton, James S.
  • Chemical Research in Toxicology, Vol. 17, Issue 8
  • DOI: 10.1021/tx049898m

Structure and Inhibition of Microbiome β-Glucuronidases Essential to the Alleviation of Cancer Drug Toxicity
journal, September 2015


Biosynthesis of glycosaminoglycans: associated disorders and biochemical tests
journal, December 2015

  • Sasarman, Florin; Maftei, Catalina; Campeau, Philippe M.
  • Journal of Inherited Metabolic Disease, Vol. 39, Issue 2
  • DOI: 10.1007/s10545-015-9903-z

Intestinal UGTs as potential modifiers of pharmacokinetics and biological responses to drugs and xenobiotics
journal, February 2007


Alleviating Cancer Drug Toxicity by Inhibiting a Bacterial Enzyme
journal, November 2010


A metagenomic  -glucuronidase uncovers a core adaptive function of the human intestinal microbiome
journal, June 2010

  • Gloux, K.; Berteau, O.; El oumami, H.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1
  • DOI: 10.1073/pnas.1000066107

Glucuronides in the gut: Sugar-driven symbioses between microbe and host
journal, April 2017

  • Pellock, Samuel J.; Redinbo, Matthew R.
  • Journal of Biological Chemistry, Vol. 292, Issue 21
  • DOI: 10.1074/jbc.R116.767434

Glycosaminoglycans and Proteoglycans
journal, February 2018


Multiple NSAID-Induced Hits Injure the Small Intestine: Underlying Mechanisms and Novel Strategies
journal, October 2012

  • Boelsterli, Urs A.; Redinbo, Matthew R.; Saitta, Kyle S.
  • Toxicological Sciences, Vol. 131, Issue 2
  • DOI: 10.1093/toxsci/kfs310

Glycomics: revealing the dynamic ecology and evolution of sugar molecules
journal, March 2016