skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Engineered shell proteins for microcompartment shell electron transfer and catalysis

Patent ·
OSTI ID:1600294

Described herein are bacterial microcompartments shell proteins modified to stably incorporate iron-sulfur clusters. Such bacterial microcompartments shell proteins exhibit redox cycling and confer electron transfer functionality to bacterial microcompartment shells.

Research Organization:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-91ER20021
Assignee:
Board of Trustees of Michigan State University (East Lansing, MI)
Patent Number(s):
10,479,999
Application Number:
15/851,382
OSTI ID:
1600294
Resource Relation:
Patent File Date: 12/21/2017
Country of Publication:
United States
Language:
English

References (54)

Bacterial microcompartments and the modular construction of microbial metabolism journal January 2015
Ferredoxin and ferredoxin-heme maquettes journal December 1996
Identification of a Unique Fe-S Cluster Binding Site in a Glycyl-Radical Type Microcompartment Shell Protein journal September 2014
The Redox Potential of Dithionite and SO-2 from Equilibrium Reactions with Flavodoxins, Methyl Viologen and Hydrogen plus Hydrogenase journal April 1978
Femtomolar Zn(II) Affinity in a Peptide-Based Ligand Designed To Model Thiolate-Rich Metalloprotein Active Sites journal December 2006
A De Novo Designed 2[4Fe-4S] Ferredoxin Mimic Mediates Electron Transfer journal November 2014
Two new high-resolution crystal structures of carboxysome pentamer proteins reveal high structural conservation of CcmL orthologs among distantly related cyanobacterial species journal August 2013
Atomic-Level Models of the Bacterial Carboxysome Shell journal February 2008
Protein Design: Toward Functional Metalloenzymes journal January 2014
Iron-Sulfur Clusters: Nature's Modular, Multipurpose Structures journal August 1997
EasySpin, a comprehensive software package for spectral simulation and analysis in EPR journal January 2006
Phaser crystallographic software journal July 2007
Accumulation of metabolic products in bacterial microcompartments patent November 2015
Bacterial microcompartments: widespread prokaryotic organelles for isolation and optimization of metabolic pathways: Bacterial microcompartments journal August 2015
Iron-sulphur clusters and the problem with oxygen journal February 2006
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method journal October 2014
Identification and Structural Analysis of a Novel Carboxysome Shell Protein with Implications for Metabolite Transport journal September 2009
Ferrozine---a new spectrophotometric reagent for iron journal June 1970
Characterization of [4Fe-4S] Cluster Vibrations and Structure in Nitrogenase Fe Protein at Three Oxidation Levels via Combined NRVS, EXAFS, and DFT Analyses journal February 2013
Synthesis and characterization of de novo designed peptides modelling the binding sites of [4Fe–4S] clusters in photosystem I journal August 2009
The structure of the 2[4Fe–4S] ferredoxin from Pseudomonas aeruginosa at 1.32-Å resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values journal April 2006
Targeting Signal for Integrating Proteins, Peptides and Biological Molecules Into Bacterial Microcompartments patent-application May 2013
Assembly of Robust Bacterial Microcompartment Shells Using Building Blocks from an Organelle of Unknown Function journal May 2014
Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis journal March 2008
[Fe4S4]- and [Fe3S4]-cluster formation in synthetic peptides journal November 2011
Bacterial microcompartments moving into a synthetic biological world journal January 2013
Comparative analysis of carboxysome shell proteins journal January 2011
The rational design and construction of a cuboidal iron-sulfur protein journal June 1997
Bacterial microcompartment shells of diverse functional types possess pentameric vertex proteins: Pentameric Structure of a Shell Protein from a Grp Microcompartment journal April 2013
Evidence that a Metabolic Microcompartment Contains and Recycles Private Cofactor Pools journal April 2013
Overview of the CCP 4 suite and current developments journal March 2011
Perspective on opportunities in industrial biotechnology in renewable chemicals journal September 2011
Constructs and Systems and Methods for Producing Microcompartments patent-application January 2015
Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor journal November 2013
Proteins Containing 4Fe-4S Clusters: An Overview journal June 1980
Structure of activated aconitase: formation of the [4Fe-4S] cluster in the crystal. journal May 1989
Protein Structures Forming the Shell of Primitive Bacterial Organelles journal August 2005
Iron-sulfur clusters: ever-expanding roles journal April 2006
Dithiothreitol, a New Protective Reagent for SH Groups * journal April 1964
Radical S -Adenosylmethionine Enzymes journal January 2014
De novo design of a non-natural fold for an iron–sulfur protein: Alpha-helical coiled-coil with a four-iron four-sulfur cluster binding site in its central core journal March 2010
Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe–4S cluster-binding site journal January 2011
Characterisation of PduS, the pdu Metabolosome Corrin Reductase, and Evidence of Substructural Organisation within the Bacterial Microcompartment journal November 2010
Structure and Function of a Bacterial Microcompartment Shell Protein Engineered to Bind a [4Fe-4S] Cluster journal January 2016
Insight into the protein and solvent contributions to the reduction potentials of [4Fe–4S]2+/+ clusters: crystal structures of the Allochromatium vinosum ferredoxin variants C57A and V13G and the homologous Escherichia coli ferredoxin journal March 2009
Crystal structure of the EutL shell protein of the ethanolamine ammonia lyase microcompartment journal May 2009
High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli journal November 2010
Coot model-building tools for molecular graphics journal November 2004
PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
XDS journal January 2010
Structure and Properties of a Synthetic Analogue of Bacterial Iron-Sulfur Proteins journal September 1972
De Novo Design of a Redox-Active Minimal Rubredoxin Mimic journal April 2005
Design and Implementation of Novel and/or Enhanced Bacterial Microcompartments for Customizing Metabolis patent-application August 2012
Short N-terminal sequences package proteins into bacterial microcompartments journal March 2010