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Title: Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification

Abstract

The aliphatic chains of fatty acids are the most prominent and potentially the highest value precursor constituents of algal biomass, and thus accurately quantifying the algal biomass total fatty acid content is a prerequisite for comparing algal strains, growth conditions, and processes. Direct, acid-catalyzed transesterification of whole microalgal biomass is a simple, effective, and widely used method to determine the fatty acid content in whole algal biomass. Such a direct transesterification procedure typically covers the following steps: first, solubilizing the lipids in the biomass matrix and then liberating the fatty acids to make these available for catalytic upgrading to fatty acid methyl esters (FAMEs), subsequent extraction into hexane, and then quantification by gas chromatography. The method we describe here requires less than 10 mg of biomass per sample and is considered high-throughput and highly accurate.

Authors:
 [1]; ORCiD logo [1]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1600124
Report Number(s):
NREL/CH-2700-70677
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; fatty acid methyl ester; fatty acids; in situ transesterification

Citation Formats

Van Wychen, Stefanie R, and Laurens, Lieve M. Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification. United States: N. p., 2017. Web. doi:10.1007/7651_2017_107.
Van Wychen, Stefanie R, & Laurens, Lieve M. Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification. United States. doi:10.1007/7651_2017_107.
Van Wychen, Stefanie R, and Laurens, Lieve M. Tue . "Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification". United States. doi:10.1007/7651_2017_107.
@article{osti_1600124,
title = {Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification},
author = {Van Wychen, Stefanie R and Laurens, Lieve M},
abstractNote = {The aliphatic chains of fatty acids are the most prominent and potentially the highest value precursor constituents of algal biomass, and thus accurately quantifying the algal biomass total fatty acid content is a prerequisite for comparing algal strains, growth conditions, and processes. Direct, acid-catalyzed transesterification of whole microalgal biomass is a simple, effective, and widely used method to determine the fatty acid content in whole algal biomass. Such a direct transesterification procedure typically covers the following steps: first, solubilizing the lipids in the biomass matrix and then liberating the fatty acids to make these available for catalytic upgrading to fatty acid methyl esters (FAMEs), subsequent extraction into hexane, and then quantification by gas chromatography. The method we describe here requires less than 10 mg of biomass per sample and is considered high-throughput and highly accurate.},
doi = {10.1007/7651_2017_107},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {12}
}

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Works referenced in this record:

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