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Title: Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

Abstract

The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or a liquid chromatography method, for a more specific monomeric sugar profile.

Authors:
 [1]; ORCiD logo [1]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1600123
Report Number(s):
NREL/CH-2700-70676
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; carbohydrate; liquid chromatography; spectrophotometry

Citation Formats

Van Wychen, Stefanie R, and Laurens, Lieve M. Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography. United States: N. p., 2017. Web. doi:10.1007/7651_2017_106.
Van Wychen, Stefanie R, & Laurens, Lieve M. Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography. United States. doi:10.1007/7651_2017_106.
Van Wychen, Stefanie R, and Laurens, Lieve M. Tue . "Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography". United States. doi:10.1007/7651_2017_106.
@article{osti_1600123,
title = {Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography},
author = {Van Wychen, Stefanie R and Laurens, Lieve M},
abstractNote = {The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or a liquid chromatography method, for a more specific monomeric sugar profile.},
doi = {10.1007/7651_2017_106},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {12}
}

Book:
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