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Title: An overview of structure, function, and regulation of pyruvate kinases

; ; ; ;  [1]
  1. UAB
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
OSTI Identifier:
Resource Type:
Journal Article
Journal Name:
Protein Sci.
Additional Journal Information:
Journal Volume: 28; Journal Issue: (10) ; 10, 2019
Country of Publication:
United States

Citation Formats

Schormann, Norbert, Hayden, Katherine L., Lee, Paul, Banerjee, Surajit, Chattopadhyay, Debasish, and Cornell). An overview of structure, function, and regulation of pyruvate kinases. United States: N. p., 2020. Web. doi:10.1002/pro.3691.
Schormann, Norbert, Hayden, Katherine L., Lee, Paul, Banerjee, Surajit, Chattopadhyay, Debasish, & Cornell). An overview of structure, function, and regulation of pyruvate kinases. United States. doi:10.1002/pro.3691.
Schormann, Norbert, Hayden, Katherine L., Lee, Paul, Banerjee, Surajit, Chattopadhyay, Debasish, and Cornell). Wed . "An overview of structure, function, and regulation of pyruvate kinases". United States. doi:10.1002/pro.3691.
title = {An overview of structure, function, and regulation of pyruvate kinases},
author = {Schormann, Norbert and Hayden, Katherine L. and Lee, Paul and Banerjee, Surajit and Chattopadhyay, Debasish and Cornell)},
abstractNote = {},
doi = {10.1002/pro.3691},
journal = {Protein Sci.},
number = (10) ; 10, 2019,
volume = 28,
place = {United States},
year = {2020},
month = {2}

Works referenced in this record:

Rethinking glycolysis: on the biochemical logic of metabolic pathways
journal, May 2012

  • Bar-Even, Arren; Flamholz, Avi; Noor, Elad
  • Nature Chemical Biology, Vol. 8, Issue 6
  • DOI: 10.1038/nchembio.971

Methicillin-resistant Staphylococcus aureus (MRSA) Pyruvate Kinase as a Target for Bis-indole Alkaloids with Antibacterial Activities
journal, October 2011

  • Zoraghi, Roya; Worrall, Liam; See, Raymond H.
  • Journal of Biological Chemistry, Vol. 286, Issue 52
  • DOI: 10.1074/jbc.M111.289033

Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
journal, December 2017

The Trypanocidal Drug Suramin and Other Trypan Blue Mimetics Are Inhibitors of Pyruvate Kinases and Bind to the Adenosine Site
journal, July 2011

  • Morgan, Hugh P.; McNae, Iain W.; Nowicki, Matthew W.
  • Journal of Biological Chemistry, Vol. 286, Issue 36
  • DOI: 10.1074/jbc.M110.212613

Pyruvate kinase activators as a therapy target: a patent review 2011-2017
journal, September 2017

Allosteric Mechanism of Pyruvate Kinase from Leishmania mexicana Uses a Rock and Lock Model
journal, February 2010

  • Morgan, Hugh P.; McNae, Iain W.; Nowicki, Matthew W.
  • Journal of Biological Chemistry, Vol. 285, Issue 17
  • DOI: 10.1074/jbc.M109.079905

Pyruvate kinase: current status of regulatory and functional properties
journal, June 2003

  • Enriqueta Muñoz, Ma.; Ponce, Elizabeth
  • Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol. 135, Issue 2
  • DOI: 10.1016/S1096-4959(03)00081-2

Evolution of glycolysis
journal, January 1993

  • Fothergill-Gilmore, Linda A.; Michels, Paul A. M.
  • Progress in Biophysics and Molecular Biology, Vol. 59, Issue 2
  • DOI: 10.1016/0079-6107(93)90001-Z

The allosteric regulation of pyruvate kinase
journal, June 1996

The Allosteric Regulation of Pyruvate Kinase: A SITE-DIRECTED MUTAGENESIS STUDY
journal, April 2000

  • Valentini, Giovanna; Chiarelli, Laurent; Fortin, Riccardo
  • Journal of Biological Chemistry, Vol. 275, Issue 24
  • DOI: 10.1074/jbc.M001870200

Pyruvate Kinase. Classes of Regulatory Isoenzymes in Mammalian Tissues
journal, August 1973

Pyruvate kinase M2 is a phosphotyrosine-binding protein
journal, March 2008

  • Christofk, Heather R.; Vander Heiden, Matthew G.; Wu, Ning
  • Nature, Vol. 452, Issue 7184
  • DOI: 10.1038/nature06667

Nuclear Translocation of the Tumor Marker Pyruvate Kinase M2 Induces Programmed Cell Death
journal, February 2007

Characterization of a mitochondrion-like organelle in Cryptosporidium parvum
journal, June 2004

The unusual mitochondrial compartment of Cryptosporidium parvum
journal, February 2005

  • Henriquez, Fiona L.; Richards, Thomas A.; Roberts, Fiona
  • Trends in Parasitology, Vol. 21, Issue 2
  • DOI: 10.1016/

Mitochondria in malaria and related parasites: ancient, diverse and streamlined
journal, September 2008

  • Mather, Michael W.; Vaidya, Akhil B.
  • Journal of Bioenergetics and Biomembranes, Vol. 40, Issue 5
  • DOI: 10.1007/s10863-008-9176-4

Mitochondrial Evolution and Functions in Malaria Parasites
journal, October 2009

A Unique Hexokinase in Cryptosporidium parvum, an Apicomplexan Pathogen Lacking the Krebs Cycle and Oxidative Phosphorylation
journal, September 2014

The cell biology of cryptosporidium infection
journal, August 2011

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth
journal, March 2008

  • Christofk, Heather R.; Vander Heiden, Matthew G.; Harris, Marian H.
  • Nature, Vol. 452, Issue 7184
  • DOI: 10.1038/nature06734

Pyruvate kinase: Function, regulation and role in cancer
journal, July 2015

PKM2, a potential target for regulating cancer
journal, August 2018

The Influence of Salts on Pyruvate Kinase from Tissues of Higher Plants
journal, July 1957

Thallium (I) activation of pyruvate kinase
journal, March 1971

M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation
journal, March 2013

  • Morgan, H. P.; O'Reilly, F. J.; Wear, M. A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 15
  • DOI: 10.1073/pnas.1217157110

The allosteric regulation of pyruvate kinase by fructose-1,6-bisphosphate
journal, February 1998

Conformational Dynamics and Allostery in Pyruvate Kinase
journal, February 2016

  • Donovan, Katherine A.; Zhu, Shaolong; Liuni, Peter
  • Journal of Biological Chemistry, Vol. 291, Issue 17
  • DOI: 10.1074/jbc.M115.676270

Pyruvate Kinase of the Hyperthermophilic Crenarchaeote Thermoproteus tenax: Physiological Role and Phylogenetic Aspects
journal, April 2000

Dichotomic Phylogenetic Tree of the Pyruvate Kinase Family
journal, August 2006

  • Oria-Hernández, Jesús; Riveros-Rosas, Héctor; Ramírez-Sílva, Leticia
  • Journal of Biological Chemistry, Vol. 281, Issue 41
  • DOI: 10.1074/jbc.M605310200

The Monovalent Cation Requirement of Rabbit Muscle Pyruvate Kinase Is Eliminated by Substitution of Lysine for Glutamate 117
journal, December 1997

  • Laughlin, L. Timothy; Reed, George H.
  • Archives of Biochemistry and Biophysics, Vol. 348, Issue 2
  • DOI: 10.1006/abbi.1997.0448

Stimulation of Trypanosoma brucei pyruvate kinase by fructose 2,6-bisphosphate
journal, December 1985

The Control of Pyruvate Kinases of Escherichia coli . II. Effectors and Regulatory Properties of the Enzyme Activated by Ribose 5-Phosphate
journal, April 1975

  • Waygood, E. Bruce; Rayman, M. Khalil; Sanwal, B. D.
  • Canadian Journal of Biochemistry, Vol. 53, Issue 4
  • DOI: 10.1139/o75-061

Structural and Regulatory Properties of Pyruvate Kinase from the Cyanobacterium Synechococcus PCC 6301
journal, April 2001

  • Knowles, Vicki L.; Smith, Catherine S.; Smith, Christopher R.
  • Journal of Biological Chemistry, Vol. 276, Issue 24
  • DOI: 10.1074/jbc.M008878200

A Novel GDP-dependent Pyruvate Kinase Isozyme from Toxoplasma gondii Localizes to Both the Apicoplast and the Mitochondrion
journal, March 2008

  • Saito, Tomoya; Nishi, Manami; Lim, Muoy I.
  • Journal of Biological Chemistry, Vol. 283, Issue 20
  • DOI: 10.1074/jbc.M709015200

Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast
journal, March 2009

Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium
journal, January 1987

  • Garcia-Olalla, C.; Garrido-Pertierra, A.
  • Biochemical Journal, Vol. 241, Issue 2
  • DOI: 10.1042/bj2410573

The Crystal Structure of Toxoplasma gondii Pyruvate Kinase 1
journal, September 2010

New Insights on the Mechanism of the K+-Independent Activity of Crenarchaeota Pyruvate Kinases
journal, March 2015

  • De la Vega-Ruíz, Gustavo; Domínguez-Ramírez, Lenin; Riveros-Rosas, Héctor
  • PLOS ONE, Vol. 10, Issue 3
  • DOI: 10.1371/journal.pone.0119233

New views on an old enzyme: allosteric regulation and evolution of archaeal pyruvate kinases
journal, April 2019

  • Johnsen, Ulrike; Reinhardt, Andreas; Landan, Giddy
  • The FEBS Journal, Vol. 286, Issue 13
  • DOI: 10.1111/febs.14837

Unusual pathways and enzymes of central carbohydrate metabolism in Archaea
journal, December 2005

Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation
journal, March 2014

  • Brasen, C.; Esser, D.; Rauch, B.
  • Microbiology and Molecular Biology Reviews, Vol. 78, Issue 1
  • DOI: 10.1128/MMBR.00041-13

Structures of pyruvate kinases display evolutionarily divergent allosteric strategies
journal, September 2014

  • Morgan, Hugh P.; Zhong, Wenhe; McNae, Iain W.
  • Royal Society Open Science, Vol. 1, Issue 1
  • DOI: 10.1098/rsos.140120

Plasmodium falciparum pyruvate kinase as a novel target for antimalarial drug-screening
journal, March 2007

  • Chan, Maurice; Tan, Doreen S. H.; Sim, T. S.
  • Travel Medicine and Infectious Disease, Vol. 5, Issue 2
  • DOI: 10.1016/j.tmaid.2006.01.015

Purification and Characterization of Pyruvate Kinase fromSchizosaccharomyces pombe:Evidence for an Unusual Quaternary Structure
journal, November 1998

  • Nairn, Jacqueline; Duncan, Doris; Gray, Lyndsey M.
  • Protein Expression and Purification, Vol. 14, Issue 2
  • DOI: 10.1006/prep.1998.0938

Molecular and Regulatory Properties of Leucoplast Pyruvate Kinase from Brassica napus (Rapeseed) Suspension Cells
journal, April 2002

  • Plaxton, William C.; Smith, Christopher R.; Knowles, Vicki L.
  • Archives of Biochemistry and Biophysics, Vol. 400, Issue 1
  • DOI: 10.1006/abbi.2002.2782

Pyruvate kinase isozymes from the green alga, Selenastrum minutum
journal, February 1989

  • Lin, Min; Turpin, David H.; Plaxton, William C.
  • Archives of Biochemistry and Biophysics, Vol. 269, Issue 1
  • DOI: 10.1016/0003-9861(89)90103-3

The DynDom3D Webserver for the Analysis of Domain Movements in Multimeric Proteins
journal, January 2016

  • Girdlestone, Christopher; Hayward, Steven
  • Journal of Computational Biology, Vol. 23, Issue 1
  • DOI: 10.1089/cmb.2015.0143

Crystal structure of Escherichia coli pyruvate kinase type I: molecular basis of the allosteric transition
journal, July 1995

Kinetic evidence for a dual cation role for muscle pyruvate kinase
journal, September 1982

The conformational change of rabbit muscle pyruvate kinase induced by activating cations and its substrates
journal, August 2010

The structure of cat muscle pyruvate kinase.
journal, March 1986

Crystallographic studies on the structure and catalytic activity of pyruvate kinase from skeletal muscle
journal, October 1987

  • Muirhead, H.; Clayden, D. A.; Cuffe, S. P.
  • Biochemical Society Transactions, Vol. 15, Issue 5
  • DOI: 10.1042/bst0150996

Structure of Rabbit Muscle Pyruvate Kinase Complexed with Mn2+, K+, and Pyruvate
journal, May 1994

  • Larsen, Todd M.; Laughlin, L. Timothy; Holden, Hazel M.
  • Biochemistry, Vol. 33, Issue 20
  • DOI: 10.1021/bi00186a033

Clustal W and Clustal X version 2.0
journal, September 2007

Ligand-Induced Domain Movement in Pyruvate Kinase: Structure of the Enzyme from Rabbit Muscle with Mg2+, K+, andl-Phospholactate at 2.7 Å Resolution
journal, September 1997

  • Larsen, Todd M.; Benning, Matthew M.; Wesenberg, Gary E.
  • Archives of Biochemistry and Biophysics, Vol. 345, Issue 2
  • DOI: 10.1006/abbi.1997.0257

On the nature of allosteric transitions: A plausible model
journal, May 1965

Mechanisms of cooperativity and allosteric regulation in proteins
journal, May 1989

The Origins of Allostery: From Personal Memories to Material for the Future
journal, May 2013

An in vitro novel mechanism of regulating the activity of pyruvate kinase M2 by thyroid hormone and fructose 1,6-bisphosphate
journal, July 1991

  • Ashizawa, Kiyoto; McPhie, Peter; Lin, Kwang Huei
  • Biochemistry, Vol. 30, Issue 29
  • DOI: 10.1021/bi00243a010

Crystal Structure of Pyruvate Kinase from Geobacillus stearothermophilus
journal, May 2008

  • Suzuki, K.; Ito, S.; Shimizu-Ibuka, A.
  • Journal of Biochemistry, Vol. 144, Issue 3
  • DOI: 10.1093/jb/mvn069

Cooperativity in Bacillus stearothermophilus pyruvate kinase
journal, March 1998

  • Lovell, Simon C.; Mullick, Abdul H.; Muirhead, Hilary
  • Journal of Molecular Biology, Vol. 276, Issue 4
  • DOI: 10.1006/jmbi.1997.1569

Characterization of the inhibition of pyruvate kinase caused by phenylalanine and phenylpyruvate in rat brain cortex
journal, April 2003

Redox regulation of pyruvate kinase M2 by cysteine oxidation and S-nitrosation
journal, October 2018

  • Mitchell, Alice Rose; Yuan, Meng; Morgan, Hugh P.
  • Biochemical Journal, Vol. 475, Issue 20
  • DOI: 10.1042/BCJ20180556

A critical review of the role of M2PYK in the Warburg effect
journal, April 2019

  • Harris, Robert A.; Fenton, Aron W.
  • Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Vol. 1871, Issue 2
  • DOI: 10.1016/j.bbcan.2019.01.004

No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis
journal, May 2011

  • Bluemlein, Katharina; Grüning, Nana-Maria; Feichtinger, René G.
  • Oncotarget, Vol. 2, Issue 5
  • DOI: 10.18632/oncotarget.278

Role of PKM2 in directing the metabolic fate of glucose in cancer: a potential therapeutic target
journal, May 2018

Intestinal glucose metabolism revisited
journal, September 2014

Comparison of the phosphofructokinase and pyruvate kinase activities of Cryptosporidium parvum, Eimeria tenella and Toxoplasma gondii
journal, February 1996

  • Denton, Helen; Brown, Samantha M. A.; Roberts, Craig W.
  • Molecular and Biochemical Parasitology, Vol. 76, Issue 1-2
  • DOI: 10.1016/0166-6851(95)02527-8

Crystal Structure of Cryptosporidium parvum Pyruvate Kinase
journal, October 2012