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Title: Correlative Surface Imaging Reveals Chemical Signatures for Bacterial Hotspots on Plant Roots

Abstract

The rhizosphere is arguably the most complex microbial habitat on earth, comprising an integrated network of plant roots, soil and a highly diverse microbial community (the rhizosphere microbiome)1. Understanding, predicting and controlling plant-microbe interactions in the rhizosphere will allow us to harness the plant microbiome as a means to increase or restore plant ecosystem productivity, improve plant responses to a wide range of environmental perturbations, and mitigate effects of climate change by designing ecosystems for long-term soil carbon storage1. To this end, it is imperative to develop new molecular approaches with high spatial resolution to capture interactions at the plant-microbe, microbe-microbe, and plant-plant interfaces. In this work, we designed an imaging sample holder that allows integrated surface imaging tools to map the same locations of a plant root-microbe interface with submicron lateral resolutions, providing novel in vivo analysis of root-microbe interactions. Specifically, confocal fluorescence microscopy, time-of-flight secondary ion mass spectrometry (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), and scanning electron microscope (SEM) were used for the first time for correlative imaging of the Brachypodium distachyon root and its interaction with Pseudomonas SW25, a typical plant growth-promoting soil bacterium. Imaging data suggest that the root surface is inhomogeneous and that the interaction betweenmore » Pseudomonas and Brachypodium roots was confined to only few spots along the sampled root segments and that the bacterial attachment spots were enriched in Na- and S-related and high-mass organic species. We conclude that attachment of the Pseudomonas cells to the root surface is outcompeted by strong root-soil mineral interactions but facilitated by formation of extracellular polymeric substances (EPS).« less

Authors:
 [1];  [1];  [2];  [2];  [1]; ORCiD logo [2]; ORCiD logo [2];  [3]; ORCiD logo [2];  [2]; ORCiD logo [2]
  1. UNIVERSITY PROGRAMS
  2. BATTELLE (PACIFIC NW LAB)
  3. China University of Geoscience (Wuhan)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1599140
Report Number(s):
PNNL-SA-149225
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analyst
Additional Journal Information:
Journal Volume: 145; Journal Issue: 2
Country of Publication:
United States
Language:
English
Subject:
Plant root, Plant growth-promoting bacteria, soil, Correlative Surface imaging, ToF-SIMS, fluorescence microscopy, SEM

Citation Formats

Liu, Wen, Huang, Liuqin, Komorek, Rachel E., Handakumbura, Pubudu, Zhou, Yadong, Hu, Dehong, Engelhard, Mark H., Jiang, Hongchen, Yu, Xiao-Ying, Jansson, Georg C., and Zhu, Zihua. Correlative Surface Imaging Reveals Chemical Signatures for Bacterial Hotspots on Plant Roots. United States: N. p., 2020. Web. doi:10.1039/C9AN01954E.
Liu, Wen, Huang, Liuqin, Komorek, Rachel E., Handakumbura, Pubudu, Zhou, Yadong, Hu, Dehong, Engelhard, Mark H., Jiang, Hongchen, Yu, Xiao-Ying, Jansson, Georg C., & Zhu, Zihua. Correlative Surface Imaging Reveals Chemical Signatures for Bacterial Hotspots on Plant Roots. United States. doi:10.1039/C9AN01954E.
Liu, Wen, Huang, Liuqin, Komorek, Rachel E., Handakumbura, Pubudu, Zhou, Yadong, Hu, Dehong, Engelhard, Mark H., Jiang, Hongchen, Yu, Xiao-Ying, Jansson, Georg C., and Zhu, Zihua. Tue . "Correlative Surface Imaging Reveals Chemical Signatures for Bacterial Hotspots on Plant Roots". United States. doi:10.1039/C9AN01954E.
@article{osti_1599140,
title = {Correlative Surface Imaging Reveals Chemical Signatures for Bacterial Hotspots on Plant Roots},
author = {Liu, Wen and Huang, Liuqin and Komorek, Rachel E. and Handakumbura, Pubudu and Zhou, Yadong and Hu, Dehong and Engelhard, Mark H. and Jiang, Hongchen and Yu, Xiao-Ying and Jansson, Georg C. and Zhu, Zihua},
abstractNote = {The rhizosphere is arguably the most complex microbial habitat on earth, comprising an integrated network of plant roots, soil and a highly diverse microbial community (the rhizosphere microbiome)1. Understanding, predicting and controlling plant-microbe interactions in the rhizosphere will allow us to harness the plant microbiome as a means to increase or restore plant ecosystem productivity, improve plant responses to a wide range of environmental perturbations, and mitigate effects of climate change by designing ecosystems for long-term soil carbon storage1. To this end, it is imperative to develop new molecular approaches with high spatial resolution to capture interactions at the plant-microbe, microbe-microbe, and plant-plant interfaces. In this work, we designed an imaging sample holder that allows integrated surface imaging tools to map the same locations of a plant root-microbe interface with submicron lateral resolutions, providing novel in vivo analysis of root-microbe interactions. Specifically, confocal fluorescence microscopy, time-of-flight secondary ion mass spectrometry (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), and scanning electron microscope (SEM) were used for the first time for correlative imaging of the Brachypodium distachyon root and its interaction with Pseudomonas SW25, a typical plant growth-promoting soil bacterium. Imaging data suggest that the root surface is inhomogeneous and that the interaction between Pseudomonas and Brachypodium roots was confined to only few spots along the sampled root segments and that the bacterial attachment spots were enriched in Na- and S-related and high-mass organic species. We conclude that attachment of the Pseudomonas cells to the root surface is outcompeted by strong root-soil mineral interactions but facilitated by formation of extracellular polymeric substances (EPS).},
doi = {10.1039/C9AN01954E},
journal = {Analyst},
number = 2,
volume = 145,
place = {United States},
year = {2020},
month = {1}
}

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