Visualizing Microbial Community Dynamics via a Controllable Soil Environment

Bhattacharjee, Arunima; Velickovic, Dusan; Wietsma, Thomas W.; Bell, Sheryl L.; Jansson, Janet K.; Hofmockel, Kirsten S.; Anderton, Christopher R.; Wilmes, ed., Paul

doi:10.1128/mSystems.00645-19

Title: Visualizing Microbial Community Dynamics via a Controllable Soil Environment

Journal Article · Tue Feb 11 00:00:00 EST 2020 · mSystems

DOI:https://doi.org/10.1128/mSystems.00645-19· OSTI ID:1598721

Bhattacharjee, Arunima; Velickovic, Dusan; Wietsma, Thomas W.; Bell, Sheryl L.; Jansson, Janet K.; Hofmockel, Kirsten S.;

; Wilmes, ed., Paul

Visualizing microbial community dynamics within soil environments remains challenging. Here, we developed a custom-built SoilBox system that facilitates probing various changes occurring in microbial community development and molecular processes when exposed to environmental perturbations (e.g., temperature changes, drought, nutrient availability). Using confocal microscopy along with mass spectrometry imaging, we demonstrate the power of this system by correlating microbial growth and spatial metabolic information from soil communities under different moisture regimes.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1598721

Alternate ID(s):: OSTI ID: 1600533

Report Number(s):: PNNL-SA-147223; e00645-19

Journal Information:: mSystems, Journal Name: mSystems Vol. 5 Journal Issue: 1; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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