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Title: Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure

Abstract

Modifying lignin composition and structure is a key strategy to increase plant cell wall digestibility for biofuel production. Disruption of the genes encoding both cinnamyl alcohol dehydrogenases (CADs), including CADC and CADD, in Arabidopsis thaliana results in the atypical incorporation of hydroxycinnamaldehydes into lignin. Another strategy to change lignin composition is downregulation or overexpression of ferulate 5-hydroxylase (F5H), which results in lignins enriched in guaiacyl or syringyl units, respectively. Here, we combined these approaches to generate plants enriched in coniferaldehyde-derived lignin units or lignins derived primarily from sinapaldehyde. The cadc cadd and ferulic acid hydroxylase1 (fah1) cadc cadd plants are similar in growth to wild-type plants even though their lignin compositions are drastically altered. In contrast, disruption of CAD in the F5H-overexpressing background results in dwarfism. The dwarfed phenotype observed in these plants does not appear to be related to collapsed xylem, a hallmark of many other lignin-deficient dwarf mutants. cadc cadd, fah1 cadc cadd, and cadd F5H-overexpressing plants have increased enzyme-catalyzed cell wall digestibility. Given that these CAD-deficient plants have similar total lignin contents and only differ in the amounts of hydroxycinnamaldehyde monomer incorporation, these results suggest that hydroxycinnamaldehyde content is a more important determinant of digestibility than ligninmore » content.« less

Authors:
ORCiD logo [1];  [2];  [3]; ORCiD logo [4];  [5]; ORCiD logo [3]; ORCiD logo [6]; ORCiD logo [7]
  1. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907; Heartland Plant Innovations, Manhattan, Kansas 66502
  2. Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706; DOE Great Lakes Bioenergy Research Center and Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin 53726; Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan
  3. Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401
  4. Department of Agricultural and Biological Engineering and the Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907
  5. Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706; DOE Great Lakes Bioenergy Research Center and Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin 53726; Department of Biological Systems Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706
  6. Department of Agricultural and Biological Engineering and the Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907
  7. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1597748
Grant/Contract Number:  
FG02-07ER15905
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Cell
Additional Journal Information:
Journal Volume: 27; Journal Issue: 8; Journal ID: ISSN 1040-4651
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Anderson, Nickolas A., Tobimatsu, Yuki, Ciesielski, Peter N., Ximenes, Eduardo, Ralph, John, Donohoe, Bryon S., Ladisch, Michael, and Chapple, Clint. Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure. United States: N. p., 2015. Web. doi:10.1105/tpc.15.00373.
Anderson, Nickolas A., Tobimatsu, Yuki, Ciesielski, Peter N., Ximenes, Eduardo, Ralph, John, Donohoe, Bryon S., Ladisch, Michael, & Chapple, Clint. Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure. United States. doi:10.1105/tpc.15.00373.
Anderson, Nickolas A., Tobimatsu, Yuki, Ciesielski, Peter N., Ximenes, Eduardo, Ralph, John, Donohoe, Bryon S., Ladisch, Michael, and Chapple, Clint. Sat . "Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure". United States. doi:10.1105/tpc.15.00373. https://www.osti.gov/servlets/purl/1597748.
@article{osti_1597748,
title = {Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure},
author = {Anderson, Nickolas A. and Tobimatsu, Yuki and Ciesielski, Peter N. and Ximenes, Eduardo and Ralph, John and Donohoe, Bryon S. and Ladisch, Michael and Chapple, Clint},
abstractNote = {Modifying lignin composition and structure is a key strategy to increase plant cell wall digestibility for biofuel production. Disruption of the genes encoding both cinnamyl alcohol dehydrogenases (CADs), including CADC and CADD, in Arabidopsis thaliana results in the atypical incorporation of hydroxycinnamaldehydes into lignin. Another strategy to change lignin composition is downregulation or overexpression of ferulate 5-hydroxylase (F5H), which results in lignins enriched in guaiacyl or syringyl units, respectively. Here, we combined these approaches to generate plants enriched in coniferaldehyde-derived lignin units or lignins derived primarily from sinapaldehyde. The cadc cadd and ferulic acid hydroxylase1 (fah1) cadc cadd plants are similar in growth to wild-type plants even though their lignin compositions are drastically altered. In contrast, disruption of CAD in the F5H-overexpressing background results in dwarfism. The dwarfed phenotype observed in these plants does not appear to be related to collapsed xylem, a hallmark of many other lignin-deficient dwarf mutants. cadc cadd, fah1 cadc cadd, and cadd F5H-overexpressing plants have increased enzyme-catalyzed cell wall digestibility. Given that these CAD-deficient plants have similar total lignin contents and only differ in the amounts of hydroxycinnamaldehyde monomer incorporation, these results suggest that hydroxycinnamaldehyde content is a more important determinant of digestibility than lignin content.},
doi = {10.1105/tpc.15.00373},
journal = {Plant Cell},
issn = {1040-4651},
number = 8,
volume = 27,
place = {United States},
year = {2015},
month = {8}
}

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Cited by: 24 works
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Works referencing / citing this record:

Lignin modification in planta for valorization
journal, January 2018


The lignin toolbox of the model grass Setaria viridis
journal, June 2019

  • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
  • Plant Molecular Biology, Vol. 101, Issue 3
  • DOI: 10.1007/s11103-019-00897-9

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production
journal, December 2016


Hydrolysis of untreated lignocellulosic feedstock is independent of S-lignin composition in newly classified anaerobic fungal isolate, Piromyces sp. UH3-1
journal, October 2018

  • Hooker, Casey A.; Hillman, Ethan T.; Overton, Jonathan C.
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1292-8

Lignin modification in planta for valorization
journal, January 2018


The lignin toolbox of the model grass Setaria viridis
journal, June 2019

  • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
  • Plant Molecular Biology, Vol. 101, Issue 3
  • DOI: 10.1007/s11103-019-00897-9

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production
journal, December 2016


Hydrolysis of untreated lignocellulosic feedstock is independent of S-lignin composition in newly classified anaerobic fungal isolate, Piromyces sp. UH3-1
journal, October 2018

  • Hooker, Casey A.; Hillman, Ethan T.; Overton, Jonathan C.
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1292-8