Coaxial capillary and conductive capillary interfaces for collection of fractions isolated by capillary electrophoresis
- Univ. of California, Riverside, CA (United States)
An instrument is described that allows the automated collection of fractions isolated by capillary electrophoresis. This instrument allows the electrical connection to be established with the separation capillary by using a coaxial capillary flow cell or by treating the outer surface of the capillary with a gold-filled epoxy to allow electrophoresis. The coaxial interface is most useful when the electroosmotic flow in the capillary is small, and the conductive capillary interface is favored when dilution and contamination of the sample must be minimized. Both geometries permit closely spaced fractions to be acquired with minimal cross-contamination and dilution. Sample recoveries were better than 80% and virtually independent of the chemical characteristics of the sample. Fractions isolated with this instrument were successfully analyzed by high-pressure liquid chromatography and electrospray mass spectrometry. 25 refs., 4 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 159685
- Journal Information:
- Analytical Chemistry (Washington), Vol. 67, Issue 22; Other Information: PBD: 15 Nov 1995
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evaluation of a sheathless capillary electrophoresis/electrospray ionization interface on an ion trap mass spectrometer
Capillary isotachophoresis mass spectrometry