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Title: Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization

Abstract

One critical aspect of mass spectrometry imaging (MSI) is the need to con?dently identify detected analytes. While orthogonal tandem MS (e.g., LC-MS2) experiments from sample extracts can assist in annotating ions, the spatial information about these molecules is lost. Accordingly, this could cause mislead conclusions, especially in cases where isobaric species exhibit di?erent distributions within a sample. In this Technical Note, we employed a multimodal imaging approach, using matrix assisted laser desorption/ionization (MALDI)-MSI and liquid extraction surface analysis (LESA)-MS2I, to con?dently annotate and One critical aspect of mass spectrometry imaging (MSI) is the need to con?dently identify detected analytes. While orthogonal tandem MS (e.g., LC-MS2) experiments from sample extracts can assist in annotating ions, the spatial information about these molecules is lost. Accordingly, this could cause mislead conclusions, especially in cases where isobaric species exhibit di?erent distributions within a sample. In this Technical Note, we employed a multimodal imaging approach, using matrix assisted laser desorption/ionization (MALDI)-MSI and liquid extraction surface analysis (LESA)-MS2I, to con?dently annotate and localize a broad range of metabolites involved in a tripartite symbiosis system of moss, cyanobacteria, and fungus. We found that the combination of these two imaging modalities generated very congruent ion images, providing themore » link between highly accurate structural information onfered by LESA and high spatial resolution attainable by MALDI. These results demonstrate how this combined methodology could be very useful in di?erentiating metabolite routes in complex systems.« less

Authors:
 [1];  [1];  [2]; ORCiD logo [1];  [1];  [3];  [1]
  1. BATTELLE (PACIFIC NW LAB)
  2. Duke University
  3. Oak Ridge National Laboratory
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1582594
Report Number(s):
PNNL-SA-132227
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 90; Journal Issue: 1
Country of Publication:
United States
Language:
English

Citation Formats

Velickovic, Dusan, Chu, Rosalie K., Carrell, Alyssa A., Thomas, Mathew, Pasa Tolic, Ljiljana, Weston, David J., and Anderton, Christopher R. Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization. United States: N. p., 2018. Web. doi:10.1021/acs.analchem.7b04319.
Velickovic, Dusan, Chu, Rosalie K., Carrell, Alyssa A., Thomas, Mathew, Pasa Tolic, Ljiljana, Weston, David J., & Anderton, Christopher R. Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization. United States. doi:10.1021/acs.analchem.7b04319.
Velickovic, Dusan, Chu, Rosalie K., Carrell, Alyssa A., Thomas, Mathew, Pasa Tolic, Ljiljana, Weston, David J., and Anderton, Christopher R. Tue . "Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization". United States. doi:10.1021/acs.analchem.7b04319.
@article{osti_1582594,
title = {Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization},
author = {Velickovic, Dusan and Chu, Rosalie K. and Carrell, Alyssa A. and Thomas, Mathew and Pasa Tolic, Ljiljana and Weston, David J. and Anderton, Christopher R.},
abstractNote = {One critical aspect of mass spectrometry imaging (MSI) is the need to con?dently identify detected analytes. While orthogonal tandem MS (e.g., LC-MS2) experiments from sample extracts can assist in annotating ions, the spatial information about these molecules is lost. Accordingly, this could cause mislead conclusions, especially in cases where isobaric species exhibit di?erent distributions within a sample. In this Technical Note, we employed a multimodal imaging approach, using matrix assisted laser desorption/ionization (MALDI)-MSI and liquid extraction surface analysis (LESA)-MS2I, to con?dently annotate and One critical aspect of mass spectrometry imaging (MSI) is the need to con?dently identify detected analytes. While orthogonal tandem MS (e.g., LC-MS2) experiments from sample extracts can assist in annotating ions, the spatial information about these molecules is lost. Accordingly, this could cause mislead conclusions, especially in cases where isobaric species exhibit di?erent distributions within a sample. In this Technical Note, we employed a multimodal imaging approach, using matrix assisted laser desorption/ionization (MALDI)-MSI and liquid extraction surface analysis (LESA)-MS2I, to con?dently annotate and localize a broad range of metabolites involved in a tripartite symbiosis system of moss, cyanobacteria, and fungus. We found that the combination of these two imaging modalities generated very congruent ion images, providing the link between highly accurate structural information onfered by LESA and high spatial resolution attainable by MALDI. These results demonstrate how this combined methodology could be very useful in di?erentiating metabolite routes in complex systems.},
doi = {10.1021/acs.analchem.7b04319},
journal = {Analytical Chemistry},
number = 1,
volume = 90,
place = {United States},
year = {2018},
month = {1}
}