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Title: Metabolism, Survival, and Gene Expression of Pseudomonas putida to Hematite Nanoparticles Mediated by Surface-Bound Humic Acid

Abstract

Natural organic matter (NOM) is likely to coat naturally occurring nanoparticles (NNPs) in the soil environment and poses distinct effects on the interaction between NPs and soil microorganisms, however such topic has not been well investigated. This study explored the influence of nanoparticle surface-bound humic acid (HA, as a model NOM) on the toxicity of hematite NPs (i.e., nano-Fe2O3) to Pseudomonas putida (P. putida). Results showed that nano-Fe2O3 could inhibit the bacterial growth with an IC50 of 23.58 mg L-1, while nanoparticle surface-bound HA could significantly alleviate the P. putida toxicity of nano-Fe2O3. IC50 of nano-Fe2O3 increased to 4774.23 mg L-1 as a result of surface-saturation by HA. Co-precipitation experiment and transmission electron microscopy observation revealed that nanoparticle surface-bound HA prevented the adhesion of nano-Fe2O3 to the cells as well as limited cell internalization of nanoparticles due to the increased electrostatic repulsion. The generation of intracellular reactive oxygen species (ROS) was significantly limited by the nanoparticle surface-bound HA. The prevention of adhesion and inhibition of ROS generation could account for the HA-mitigated nanotoxicity. Interfacial interactions between hematite NPs and cell membrane were also evaluated on the basis of the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, and the magnitude of interaction energy barrier correlatedmore » well with the 48 h LC50 data of hematite NPs to P. putida. This result implies that metal oxide NPs with strong association with the cell surface might induce more severe cytotoxicity in microorganisms.« less

Authors:
 [1];  [2]; ORCiD logo [3];  [1];  [1];  [4]
  1. Huazhong Agricultural University
  2. University of California, Riverside
  3. BATTELLE (PACIFIC NW LAB)
  4. Huazhaong Agricultual University
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1581783
Report Number(s):
PNNL-SA-121317
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Environmental Science Nano
Additional Journal Information:
Journal Volume: 5; Journal Issue: 3
Country of Publication:
United States
Language:
English
Subject:
natural occurring nanoparticles, na, hematite, su, Pseudomonas putida, nanotoxicity

Citation Formats

Ouyang, Kai, Walker, Sharon L., Yu, Xiao-Ying, Gao, Chun-Hui, Huang, Qiaoyun, and Cai, Peng. Metabolism, Survival, and Gene Expression of Pseudomonas putida to Hematite Nanoparticles Mediated by Surface-Bound Humic Acid. United States: N. p., 2018. Web. doi:10.1039/C7EN01039G.
Ouyang, Kai, Walker, Sharon L., Yu, Xiao-Ying, Gao, Chun-Hui, Huang, Qiaoyun, & Cai, Peng. Metabolism, Survival, and Gene Expression of Pseudomonas putida to Hematite Nanoparticles Mediated by Surface-Bound Humic Acid. United States. doi:10.1039/C7EN01039G.
Ouyang, Kai, Walker, Sharon L., Yu, Xiao-Ying, Gao, Chun-Hui, Huang, Qiaoyun, and Cai, Peng. Thu . "Metabolism, Survival, and Gene Expression of Pseudomonas putida to Hematite Nanoparticles Mediated by Surface-Bound Humic Acid". United States. doi:10.1039/C7EN01039G.
@article{osti_1581783,
title = {Metabolism, Survival, and Gene Expression of Pseudomonas putida to Hematite Nanoparticles Mediated by Surface-Bound Humic Acid},
author = {Ouyang, Kai and Walker, Sharon L. and Yu, Xiao-Ying and Gao, Chun-Hui and Huang, Qiaoyun and Cai, Peng},
abstractNote = {Natural organic matter (NOM) is likely to coat naturally occurring nanoparticles (NNPs) in the soil environment and poses distinct effects on the interaction between NPs and soil microorganisms, however such topic has not been well investigated. This study explored the influence of nanoparticle surface-bound humic acid (HA, as a model NOM) on the toxicity of hematite NPs (i.e., nano-Fe2O3) to Pseudomonas putida (P. putida). Results showed that nano-Fe2O3 could inhibit the bacterial growth with an IC50 of 23.58 mg L-1, while nanoparticle surface-bound HA could significantly alleviate the P. putida toxicity of nano-Fe2O3. IC50 of nano-Fe2O3 increased to 4774.23 mg L-1 as a result of surface-saturation by HA. Co-precipitation experiment and transmission electron microscopy observation revealed that nanoparticle surface-bound HA prevented the adhesion of nano-Fe2O3 to the cells as well as limited cell internalization of nanoparticles due to the increased electrostatic repulsion. The generation of intracellular reactive oxygen species (ROS) was significantly limited by the nanoparticle surface-bound HA. The prevention of adhesion and inhibition of ROS generation could account for the HA-mitigated nanotoxicity. Interfacial interactions between hematite NPs and cell membrane were also evaluated on the basis of the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, and the magnitude of interaction energy barrier correlated well with the 48 h LC50 data of hematite NPs to P. putida. This result implies that metal oxide NPs with strong association with the cell surface might induce more severe cytotoxicity in microorganisms.},
doi = {10.1039/C7EN01039G},
journal = {Environmental Science Nano},
number = 3,
volume = 5,
place = {United States},
year = {2018},
month = {3}
}

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