Maintenance of Sympatric and Allopatric Populations in Free-Living Terrestrial Bacteria

Chase, Alexander B.; Arevalo, Philip; Brodie, Eoin L.; Polz, Martin F.; Karaoz, Ulas; Martiny, Jennifer B.  H.; Taylor, John W.

doi:10.1128/mBio.02361-19

Title: Maintenance of Sympatric and Allopatric Populations in Free-Living Terrestrial Bacteria

Journal Article · Tue Oct 29 00:00:00 EDT 2019 · mBio (Online)

DOI:https://doi.org/10.1128/mBio.02361-19· OSTI ID:1581756

^[1];

^[2]; Brodie, Eoin L. ^[3];

^[2];

^[4]; Martiny, Jennifer B. H. ^[5]; Taylor, John W. ^[6]

Univ. of California, Irvine, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Irvine, CA (United States)
Univ. of California, Berkeley, CA (United States)

For free-living bacteria and archaea, the equivalent of the biological species concept does not exist, creating several obstacles to the study of the processes contributing to microbial diversification. These obstacles are particularly high in soil, where high bacterial diversity inhibits the study of closely related genotypes and therefore the factors structuring microbial populations. Here, we isolated strains within a singleCurtobacteriumecotype from surface soil (leaf litter) across a regional climate gradient and investigated the phylogenetic structure, recombination, and flexible gene content of this genomic diversity to infer patterns of gene flow. Our results indicate that microbial populations are delineated by gene flow discontinuities, with distinct populations cooccurring at multiple sites. Bacterial population structure was further delineated by genomic features allowing for the identification of candidate genes possibly contributing to local adaptation. These results suggest that the genetic structure within this bacterium is maintained both by ecological specialization in localized microenvironments (isolation by environment) and by dispersal limitation between geographic locations (isolation by distance).

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; SC0016410

OSTI ID:: 1581756

Journal Information:: mBio (Online), Vol. 10, Issue 5; ISSN 2150-7511

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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Title: Maintenance of Sympatric and Allopatric Populations in Free-Living Terrestrial Bacteria

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