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Title: Microbial dynamics in anaerobic digestion reactors for treating organic urban residues during the start-up process

Abstract

Anaerobic digestion of organic residues offers economic benefits via biogas production, still methane (CH 4) yield relies on the development of a robust microbial consortia for adequate substrate degradation, among other factors. In this study, we monitor biogas production and changes in the microbial community composition in two semi-continuous stirred tank reactors during the setting process under mesophilic conditions (35°C) using a 16S rDNA high-throughput sequencing method. Reactors were initially inoculated with anaerobic granular sludge from a brewery wastewater treatment plant, and gradually fed organic urban residues (4·0 kg VS m -3 day -1) . The inocula and biomass samples showed changes related to adaptations of the community to urban organic wastes including a higher relative proportion of Clostridiales, with Ruminococcus spp. and Syntrophomonas spp. as recurrent species. Candidatus Cloacamonas spp. (Spirochaetes) also increased from ~2·2% in the inoculum to >10% in the reactor biomass. The new community consolidated the cellulose degradation and the propionate and amino acids fermentation processes. Acetoclastic methanogens were more abundant in the reactor, where Methanosaeta spp. was found as a key player. This study demonstrates a successful use of brewery treatment plant granular sludge to obtain a robust consortium for methane production from urban organic solid waste inmore » Mexico.This study describes the selection of relevant bacteria and archaea in anaerobic digesters inoculated with anaerobic granular sludge from a brewery wastewater treatment plant. Generally, these sludge granules are used to inoculate reactors digesting organic urban wastes. Though, it is still not clearly understood how micro-organisms respond to substrate variations during the reactor start-up process. After feeding two reactors with organic urban residues, it was found that a broader potential for cellulose degradation was developed including Bacteroidetes, Firmicutes and Spirochaetes. These results clarify the bacterial processes behind new reactors establishment for treating organic wastes in urban areas.« less

Authors:
ORCiD logo [1];  [2];  [3];  [3];  [4]
  1. Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico). Inst. de Geology
  2. Lawrence Berkeley National Laboratory, Climate and Ecosystem Sciences, Ecology Department, Berkeley CA USA
  3. Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico).Dept. de Ingeniería Química
  4. Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico). Lab. de Ecología Bacteriana, Inst. de Ecología
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1580089
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Letters in Applied Microbiology
Additional Journal Information:
Journal Volume: 64; Journal Issue: 6; Journal ID: ISSN 0266-8254
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Alcántara-Hernández, R. J., Taş, N., Carlos-Pinedo, S., Durán-Moreno, A., and Falcón, L. I. Microbial dynamics in anaerobic digestion reactors for treating organic urban residues during the start-up process. United States: N. p., 2017. Web. doi:10.1111/lam.12734.
Alcántara-Hernández, R. J., Taş, N., Carlos-Pinedo, S., Durán-Moreno, A., & Falcón, L. I. Microbial dynamics in anaerobic digestion reactors for treating organic urban residues during the start-up process. United States. https://doi.org/10.1111/lam.12734
Alcántara-Hernández, R. J., Taş, N., Carlos-Pinedo, S., Durán-Moreno, A., and Falcón, L. I. Mon . "Microbial dynamics in anaerobic digestion reactors for treating organic urban residues during the start-up process". United States. https://doi.org/10.1111/lam.12734. https://www.osti.gov/servlets/purl/1580089.
@article{osti_1580089,
title = {Microbial dynamics in anaerobic digestion reactors for treating organic urban residues during the start-up process},
author = {Alcántara-Hernández, R. J. and Taş, N. and Carlos-Pinedo, S. and Durán-Moreno, A. and Falcón, L. I.},
abstractNote = {Anaerobic digestion of organic residues offers economic benefits via biogas production, still methane (CH4) yield relies on the development of a robust microbial consortia for adequate substrate degradation, among other factors. In this study, we monitor biogas production and changes in the microbial community composition in two semi-continuous stirred tank reactors during the setting process under mesophilic conditions (35°C) using a 16S rDNA high-throughput sequencing method. Reactors were initially inoculated with anaerobic granular sludge from a brewery wastewater treatment plant, and gradually fed organic urban residues (4·0 kg VS m-3 day-1) . The inocula and biomass samples showed changes related to adaptations of the community to urban organic wastes including a higher relative proportion of Clostridiales, with Ruminococcus spp. and Syntrophomonas spp. as recurrent species. Candidatus Cloacamonas spp. (Spirochaetes) also increased from ~2·2% in the inoculum to >10% in the reactor biomass. The new community consolidated the cellulose degradation and the propionate and amino acids fermentation processes. Acetoclastic methanogens were more abundant in the reactor, where Methanosaeta spp. was found as a key player. This study demonstrates a successful use of brewery treatment plant granular sludge to obtain a robust consortium for methane production from urban organic solid waste in Mexico.This study describes the selection of relevant bacteria and archaea in anaerobic digesters inoculated with anaerobic granular sludge from a brewery wastewater treatment plant. Generally, these sludge granules are used to inoculate reactors digesting organic urban wastes. Though, it is still not clearly understood how micro-organisms respond to substrate variations during the reactor start-up process. After feeding two reactors with organic urban residues, it was found that a broader potential for cellulose degradation was developed including Bacteroidetes, Firmicutes and Spirochaetes. These results clarify the bacterial processes behind new reactors establishment for treating organic wastes in urban areas.},
doi = {10.1111/lam.12734},
url = {https://www.osti.gov/biblio/1580089}, journal = {Letters in Applied Microbiology},
issn = {0266-8254},
number = 6,
volume = 64,
place = {United States},
year = {2017},
month = {3}
}

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Cited by: 13 works
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Works referenced in this record:

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    Long-Term Evaluation of Mesophilic Semi-Continuous Anaerobic Digestion of Olive Mill Solid Waste Pretreated with Steam-Explosion
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    Biogas production and microbial community structure in a stable‐stage of a two‐stage anaerobic digester
    journal, October 2019


    Mapping anaerobic sludge bed community adaptations to manure supernatant in biogas reactors
    journal, October 2018