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Title: Improvement of antibody functionality by structure-guided paratope engraftment


Citation Formats

Liu, Qingbo, Lai, Yen-Ting, Zhang, Peng, Louder, Mark K., Pegu, Amarendra, Rawi, Reda, Asokan, Mangaiarkarasi, Chen, Xuejun, Shen, Chen-Hsiang, Chuang, Gwo-Yu, Yang, Eun Sung, Miao, Huiyi, Wang, Yuge, Fauci, Anthony S., Kwong, Peter D., Mascola, John R., and Lusso, Paolo. Improvement of antibody functionality by structure-guided paratope engraftment. United States: N. p., 2019. Web. doi:10.1038/s41467-019-08658-4.
Liu, Qingbo, Lai, Yen-Ting, Zhang, Peng, Louder, Mark K., Pegu, Amarendra, Rawi, Reda, Asokan, Mangaiarkarasi, Chen, Xuejun, Shen, Chen-Hsiang, Chuang, Gwo-Yu, Yang, Eun Sung, Miao, Huiyi, Wang, Yuge, Fauci, Anthony S., Kwong, Peter D., Mascola, John R., & Lusso, Paolo. Improvement of antibody functionality by structure-guided paratope engraftment. United States. doi:10.1038/s41467-019-08658-4.
Liu, Qingbo, Lai, Yen-Ting, Zhang, Peng, Louder, Mark K., Pegu, Amarendra, Rawi, Reda, Asokan, Mangaiarkarasi, Chen, Xuejun, Shen, Chen-Hsiang, Chuang, Gwo-Yu, Yang, Eun Sung, Miao, Huiyi, Wang, Yuge, Fauci, Anthony S., Kwong, Peter D., Mascola, John R., and Lusso, Paolo. Wed . "Improvement of antibody functionality by structure-guided paratope engraftment". United States. doi:10.1038/s41467-019-08658-4.
@article{osti_1577154,
title = {Improvement of antibody functionality by structure-guided paratope engraftment},
author = {Liu, Qingbo and Lai, Yen-Ting and Zhang, Peng and Louder, Mark K. and Pegu, Amarendra and Rawi, Reda and Asokan, Mangaiarkarasi and Chen, Xuejun and Shen, Chen-Hsiang and Chuang, Gwo-Yu and Yang, Eun Sung and Miao, Huiyi and Wang, Yuge and Fauci, Anthony S. and Kwong, Peter D. and Mascola, John R. and Lusso, Paolo},
abstractNote = {},
doi = {10.1038/s41467-019-08658-4},
journal = {Nature Communications},
issn = {2041-1723},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {2}
}

Works referenced in this record:

Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses
journal, July 2016


An Insertion Mutation That Distorts Antibody Binding Site Architecture Enhances Function of a Human Antibody
journal, February 2011

  • Krause, Jens C.; Ekiert, Damian C.; Tumpey, Terrence M.
  • mBio, Vol. 2, Issue 1
  • DOI: 10.1128/mBio.00345-10

Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1
journal, July 2014

  • Sarzotti-Kelsoe, Marcella; Bailer, Robert T.; Turk, Ellen
  • Journal of Immunological Methods, Vol. 409
  • DOI: 10.1016/j.jim.2013.11.022

Gene-Specific Substitution Profiles Describe the Types and Frequencies of Amino Acid Changes during Antibody Somatic Hypermutation
journal, May 2017


Enhanced Potency of a Broadly Neutralizing HIV-1 Antibody In Vitro Improves Protection against Lentiviral Infection In Vivo
journal, August 2014

  • Rudicell, R. S.; Kwon, Y. D.; Ko, S. -Y.
  • Journal of Virology, Vol. 88, Issue 21
  • DOI: 10.1128/JVI.02213-14

Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth
journal, November 2016


Broad and potent HIV-1 neutralization by a human antibody that binds the gp41–gp120 interface
journal, September 2014

  • Huang, Jinghe; Kang, Byong H.; Pancera, Marie
  • Nature, Vol. 515, Issue 7525
  • DOI: 10.1038/nature13601

A functional antibody mutant with an insertion in the framework region 3 loop of the V H domain: implications for antibody engineering
journal, January 1992

  • Simon, Thomas; Rajewsky, Klaus
  • "Protein Engineering, Design and Selection", Vol. 5, Issue 3
  • DOI: 10.1093/protein/5.3.229

Bispecific Anti-HIV-1 Antibodies with Enhanced Breadth and Potency
journal, June 2016


Global Panel of HIV-1 Env Reference Strains for Standardized Assessments of Vaccine-Elicited Neutralizing Antibodies
journal, December 2013

  • deCamp, A.; Hraber, P.; Bailer, R. T.
  • Journal of Virology, Vol. 88, Issue 5
  • DOI: 10.1128/JVI.02853-13

Engineered Bispecific Antibodies with Exquisite HIV-1-Neutralizing Activity
journal, June 2016


HIV-1 neutralizing antibodies: understanding nature's pathways
journal, June 2013

  • Mascola, John R.; Haynes, Barton F.
  • Immunological Reviews, Vol. 254, Issue 1
  • DOI: 10.1111/imr.12075

Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding
journal, July 2011


Immune-Correlates Analysis of an HIV-1 Vaccine Efficacy Trial
journal, April 2012

  • Haynes, Barton F.; Gilbert, Peter B.; McElrath, M. Juliana
  • New England Journal of Medicine, Vol. 366, Issue 14
  • DOI: 10.1056/NEJMoa1113425

HIV-1 Vaccines Based on Antibody Identification, B Cell Ontogeny, and Epitope Structure
journal, May 2018


Cardiolipin Polyspecific Autoreactivity in Two Broadly Neutralizing HIV-1 Antibodies
journal, June 2005


Antibodies in HIV-1 Vaccine Development and Therapy
journal, September 2013


Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1
journal, June 2018


Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer
journal, February 2017

  • Liu, Qingbo; Acharya, Priyamvada; Dolan, Michael A.
  • Nature Structural & Molecular Biology, Vol. 24, Issue 4
  • DOI: 10.1038/nsmb.3382

Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody
journal, May 2016


Trispecific broadly neutralizing HIV antibodies mediate potent SHIV protection in macaques
journal, September 2017


Lattice engineering enables definition of molecular features allowing for potent small-molecule inhibition of HIV-1 entry
journal, January 2019


Broadly Neutralizing Antibodies Present New Prospects to Counter Highly Antigenically Diverse Viruses
journal, July 2012


Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer
journal, October 2013


Immunoglobulin Gene Insertions and Deletions in the Affinity Maturation of HIV-1 Broadly Reactive Neutralizing Antibodies
journal, September 2014


Somatic Mutations of the Immunoglobulin Framework Are Generally Required for Broad and Potent HIV-1 Neutralization
journal, March 2013


Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors
journal, June 2015


Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1
journal, July 2010


Half-life of polyreactive antibodies
journal, March 1994

  • Sigounas, George; Harindranath, Nagaradona; Donadel, Giulia
  • Journal of Clinical Immunology, Vol. 14, Issue 2
  • DOI: 10.1007/BF01541346

Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies
journal, November 2009

  • Seaman, M. S.; Janes, H.; Hawkins, N.
  • Journal of Virology, Vol. 84, Issue 3
  • DOI: 10.1128/JVI.02108-09

Secondary mechanisms of diversification in the human antibody repertoire
journal, January 2013


Maturation and Diversity of the VRC01-Antibody Lineage over 15 Years of Chronic HIV-1 Infection
journal, April 2015


Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody
journal, April 2016


IgBLAST: an immunoglobulin variable domain sequence analysis tool
journal, May 2013

  • Ye, Jian; Ma, Ning; Madden, Thomas L.
  • Nucleic Acids Research, Vol. 41, Issue W1
  • DOI: 10.1093/nar/gkt382

SeqFeatR for the Discovery of Feature-Sequence Associations
journal, January 2016


Lessons from HIV-1 vaccine efficacy trials
journal, January 2016


Bispecific Antibodies Targeting Different Epitopes on the HIV-1 Envelope Exhibit Broad and Potent Neutralization
journal, October 2015

  • Asokan, M.; Rudicell, R. S.; Louder, M.
  • Journal of Virology, Vol. 89, Issue 24
  • DOI: 10.1128/JVI.02097-15