Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats

Xu, Qingping; Biancalana, Matthew; Wilson, Ian

doi:10.1002/pro.3683

Title: Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats

Journal Article · Sun Sep 01 00:00:00 EDT 2019 · Protein Science

DOI:https://doi.org/10.1002/pro.3683· OSTI ID:1574395

Xu, Qingping; Biancalana, Matthew; Wilson, Ian

Free-standing single-layer beta-sheets are extremely rare in naturally occurring proteins, even though beta-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel beta-sheet proteins, comprised of three or four twisted beta-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of beta-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the beta-sheet. These new modular single-layer beta-sheet proteins may serve as a new model system for studying folding and design of beta-rich proteins.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH)

DOE Contract Number:: AC02-06CH11357

OSTI ID:: 1574395

Journal Information:: Protein Science, Vol. 28, Issue 9

Country of Publication:: United States

Language:: English

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