Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats
Abstract
Free-standing single-layer beta-sheets are extremely rare in naturally occurring proteins, even though beta-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel beta-sheet proteins, comprised of three or four twisted beta-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of beta-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the beta-sheet. These new modular single-layer beta-sheet proteins may serve as a new model system for studying folding and design of beta-rich proteins.
- Authors:
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Institutes of Health (NIH)
- OSTI Identifier:
- 1574395
- DOE Contract Number:
- AC02-06CH11357
- Resource Type:
- Journal Article
- Journal Name:
- Protein Science
- Additional Journal Information:
- Journal Volume: 28; Journal Issue: 9
- Country of Publication:
- United States
- Language:
- English
- Subject:
- beta-hairpin repeats; human gut microbiome; protein folding; secreted proteins; single-layer beta-sheet proteins; structural genomics
Citation Formats
Xu, Qingping, Biancalana, Matthew, and Wilson, Ian. Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats. United States: N. p., 2019.
Web. doi:10.1002/pro.3683.
Xu, Qingping, Biancalana, Matthew, & Wilson, Ian. Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats. United States. doi:10.1002/pro.3683.
Xu, Qingping, Biancalana, Matthew, and Wilson, Ian. Sun .
"Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats". United States. doi:10.1002/pro.3683.
@article{osti_1574395,
title = {Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats},
author = {Xu, Qingping and Biancalana, Matthew and Wilson, Ian},
abstractNote = {Free-standing single-layer beta-sheets are extremely rare in naturally occurring proteins, even though beta-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel beta-sheet proteins, comprised of three or four twisted beta-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of beta-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the beta-sheet. These new modular single-layer beta-sheet proteins may serve as a new model system for studying folding and design of beta-rich proteins.},
doi = {10.1002/pro.3683},
journal = {Protein Science},
number = 9,
volume = 28,
place = {United States},
year = {2019},
month = {9}
}
DOI: 10.1002/pro.3683
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