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Title: Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats

Abstract

Free-standing single-layer beta-sheets are extremely rare in naturally occurring proteins, even though beta-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel beta-sheet proteins, comprised of three or four twisted beta-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of beta-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the beta-sheet. These new modular single-layer beta-sheet proteins may serve as a new model system for studying folding and design of beta-rich proteins.

Authors:
; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1574395
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Protein Science
Additional Journal Information:
Journal Volume: 28; Journal Issue: 9
Country of Publication:
United States
Language:
English
Subject:
beta-hairpin repeats; human gut microbiome; protein folding; secreted proteins; single-layer beta-sheet proteins; structural genomics

Citation Formats

Xu, Qingping, Biancalana, Matthew, and Wilson, Ian. Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats. United States: N. p., 2019. Web. doi:10.1002/pro.3683.
Xu, Qingping, Biancalana, Matthew, & Wilson, Ian. Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats. United States. doi:10.1002/pro.3683.
Xu, Qingping, Biancalana, Matthew, and Wilson, Ian. Sun . "Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats". United States. doi:10.1002/pro.3683.
@article{osti_1574395,
title = {Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats},
author = {Xu, Qingping and Biancalana, Matthew and Wilson, Ian},
abstractNote = {Free-standing single-layer beta-sheets are extremely rare in naturally occurring proteins, even though beta-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel beta-sheet proteins, comprised of three or four twisted beta-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of beta-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the beta-sheet. These new modular single-layer beta-sheet proteins may serve as a new model system for studying folding and design of beta-rich proteins.},
doi = {10.1002/pro.3683},
journal = {Protein Science},
number = 9,
volume = 28,
place = {United States},
year = {2019},
month = {9}
}

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