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Title: In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors

Abstract

The ability to monitor gene expression within living plants is of importance in many applications ranging from plant biology research to biofuel development; however, no method currently exists without requiring sample extraction. Herein, we report a multimodal imaging method based on plasmonic nanoprobes for in vivo imaging and biosensing of microRNA biotargets within whole plant leaves. This method integrates three different but complementary techniques: surfaceenhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The multimodal method utilizes plasmonic nanostars, which not only provide large Raman signal enhancement, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL, owing to gold content and high two-photon luminescence cross-sections. For the sensing mechanism, inverse molecular sentinel (iMS) nanoprobes are used for SERS bioimaging of microRNA within Arabidopsis thaliana leaves to provide a dynamic SERS map of detected microRNA targets while also quantifying nanoprobe concentrations using XRF and TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants, a tool that will allow the study of these biotargets with previously unmet spatial and temporal resolution.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
Argonne National Laboratory
OSTI Identifier:
1573916
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Conference
Resource Relation:
Conference: 2019 SPIE Defense and Commercial Sensing, 04/14/19 - 04/18/19, Baltimore, MD, US
Country of Publication:
United States
Language:
English
Subject:
bioimaging; gold nanostar; microRNA; plasmonics; surface enhanced Raman scattering

Citation Formats

Crawford, Bridget M., Strobbia, Pietro, Wang, Hsin-Neng, Zentella, Rodolfo, Boyanov, Maxim I., Odion, Ren, Pei, Zhen-Ming, Sun, Tai-Ping, Kemner, Kenneth M., and Vo-Dinh, Tuan. In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors. United States: N. p., 2019. Web. doi:10.1117/12.2524840.
Crawford, Bridget M., Strobbia, Pietro, Wang, Hsin-Neng, Zentella, Rodolfo, Boyanov, Maxim I., Odion, Ren, Pei, Zhen-Ming, Sun, Tai-Ping, Kemner, Kenneth M., & Vo-Dinh, Tuan. In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors. United States. doi:10.1117/12.2524840.
Crawford, Bridget M., Strobbia, Pietro, Wang, Hsin-Neng, Zentella, Rodolfo, Boyanov, Maxim I., Odion, Ren, Pei, Zhen-Ming, Sun, Tai-Ping, Kemner, Kenneth M., and Vo-Dinh, Tuan. Fri . "In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors". United States. doi:10.1117/12.2524840.
@article{osti_1573916,
title = {In vivo nucleic acid detection and imaging within whole plants using plasmonic nanosensors},
author = {Crawford, Bridget M. and Strobbia, Pietro and Wang, Hsin-Neng and Zentella, Rodolfo and Boyanov, Maxim I. and Odion, Ren and Pei, Zhen-Ming and Sun, Tai-Ping and Kemner, Kenneth M. and Vo-Dinh, Tuan},
abstractNote = {The ability to monitor gene expression within living plants is of importance in many applications ranging from plant biology research to biofuel development; however, no method currently exists without requiring sample extraction. Herein, we report a multimodal imaging method based on plasmonic nanoprobes for in vivo imaging and biosensing of microRNA biotargets within whole plant leaves. This method integrates three different but complementary techniques: surfaceenhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The multimodal method utilizes plasmonic nanostars, which not only provide large Raman signal enhancement, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL, owing to gold content and high two-photon luminescence cross-sections. For the sensing mechanism, inverse molecular sentinel (iMS) nanoprobes are used for SERS bioimaging of microRNA within Arabidopsis thaliana leaves to provide a dynamic SERS map of detected microRNA targets while also quantifying nanoprobe concentrations using XRF and TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants, a tool that will allow the study of these biotargets with previously unmet spatial and temporal resolution.},
doi = {10.1117/12.2524840},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}

Conference:
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