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Title: Fluorescent Amplification of Next Generation Sequencing Libraries Tools (FA-NGS tools) v1

Abstract

This is a software utility for calculating pooling volumes and graphing quality control data of next generation sequencing libraries amplified with fluorescent intercalating agents and monitored by real-time quantitative PCR (qPCR). The tool is used to calculate pooling volumes of individual next generation sequencing libraries based on experimentally determined fluorescence values. To do this, fluorescent values are used as a proxy for relative concentration to determine the ratio of transfer volumes. The output is a "transfer file" that specifies the transfer volume and destination well of a given library. The tool can also be used to assess the quality of individual next generation sequencing libraries by melting curve analysis. The output are small-multiples graphs of the melting curves of individual sequencing libraries according to the specified plate layout.

Developers:
 [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Release Date:
Project Type:
Open Source, Publicly Available Repository
Software Type:
Scientific
Licenses:
BSD 3-clause "New" or "Revised" License
Sponsoring Org.:
USDOE

Primary Award/Contract Number:
AC02-05CH11231
Code ID:
31391
Site Accession Number:
2019-158
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Country of Origin:
United States

Citation Formats

Garber, Megan, and USDOE. Fluorescent Amplification of Next Generation Sequencing Libraries Tools (FA-NGS tools) v1. Computer software. https://www.osti.gov//servlets/purl/1571176. USDOE. 2 Oct. 2019. Web. doi:10.11578/dc.20191021.1.
Garber, Megan, & USDOE. (2019, October 2). Fluorescent Amplification of Next Generation Sequencing Libraries Tools (FA-NGS tools) v1 [Computer software]. https://www.osti.gov//servlets/purl/1571176. doi:10.11578/dc.20191021.1.
Garber, Megan, and USDOE. Fluorescent Amplification of Next Generation Sequencing Libraries Tools (FA-NGS tools) v1. Computer software. October 2, 2019. https://www.osti.gov//servlets/purl/1571176. doi:10.11578/dc.20191021.1.
@misc{osti_1571176,
title = {Fluorescent Amplification of Next Generation Sequencing Libraries Tools (FA-NGS tools) v1},
author = {Garber, Megan and USDOE},
abstractNote = {This is a software utility for calculating pooling volumes and graphing quality control data of next generation sequencing libraries amplified with fluorescent intercalating agents and monitored by real-time quantitative PCR (qPCR). The tool is used to calculate pooling volumes of individual next generation sequencing libraries based on experimentally determined fluorescence values. To do this, fluorescent values are used as a proxy for relative concentration to determine the ratio of transfer volumes. The output is a "transfer file" that specifies the transfer volume and destination well of a given library. The tool can also be used to assess the quality of individual next generation sequencing libraries by melting curve analysis. The output are small-multiples graphs of the melting curves of individual sequencing libraries according to the specified plate layout.},
url = {https://www.osti.gov//servlets/purl/1571176},
doi = {10.11578/dc.20191021.1},
year = {2019},
month = {10},
note =
}

Software:

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