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Title: Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States)

Abstract

This study examined diel shifts in metabolic functions of Microcystis spp. during a 48-h Lagrangian survey of a toxin-producing cyanobacterial bloom in western Lake Erie in the aftermath of the 2014 Toledo Water Crisis. Transcripts mapped to the genomes of recently sequenced lower Great Lakes Microcystis isolates showed distinct patterns of gene expression between samples collected across day (10:00 h, 16:00 h) and night (22:00 h, 04:00 h). Daytime transcripts were enriched in functions related to Photosystem II (e.g., psbA), nitrogen and phosphate acquisition, cell division ( ftsHZ), heat shock response ( dnaK, groEL), and uptake of inorganic carbon ( rbc, bicA). Genes transcribed during nighttime included those involved in phycobilisome protein synthesis and Photosystem I core subunits. Hierarchical clustering and principal component analysis (PCA) showed a tightly clustered group of nighttime expressed genes, whereas daytime transcripts were separated from each other over the 48-h duration. Lack of uniform clustering within the daytime transcripts suggested that the partitioning of gene expression in Microcystis is dependent on both circadian regulation and physicochemical changes within the environment.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Biological Systems Science Division (SC-23.2 )
OSTI Identifier:
1571136
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English

Citation Formats

Davenport, Emily J., Neudeck, Michelle J., Matson, Paul G., Bullerjahn, George S., Davis, Timothy W., Wilhelm, Steven W., Denney, Maddie K., Krausfeldt, Lauren E., Stough, Joshua M. A., Meyer, Kevin A., Dick, Gregory J., Johengen, Thomas H., Lindquist, Erika, Tringe, Susannah G., and McKay, Robert Michael L. Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States). United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.02081.
Davenport, Emily J., Neudeck, Michelle J., Matson, Paul G., Bullerjahn, George S., Davis, Timothy W., Wilhelm, Steven W., Denney, Maddie K., Krausfeldt, Lauren E., Stough, Joshua M. A., Meyer, Kevin A., Dick, Gregory J., Johengen, Thomas H., Lindquist, Erika, Tringe, Susannah G., & McKay, Robert Michael L. Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States). United States. doi:10.3389/fmicb.2019.02081.
Davenport, Emily J., Neudeck, Michelle J., Matson, Paul G., Bullerjahn, George S., Davis, Timothy W., Wilhelm, Steven W., Denney, Maddie K., Krausfeldt, Lauren E., Stough, Joshua M. A., Meyer, Kevin A., Dick, Gregory J., Johengen, Thomas H., Lindquist, Erika, Tringe, Susannah G., and McKay, Robert Michael L. Tue . "Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States)". United States. doi:10.3389/fmicb.2019.02081.
@article{osti_1571136,
title = {Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States)},
author = {Davenport, Emily J. and Neudeck, Michelle J. and Matson, Paul G. and Bullerjahn, George S. and Davis, Timothy W. and Wilhelm, Steven W. and Denney, Maddie K. and Krausfeldt, Lauren E. and Stough, Joshua M. A. and Meyer, Kevin A. and Dick, Gregory J. and Johengen, Thomas H. and Lindquist, Erika and Tringe, Susannah G. and McKay, Robert Michael L.},
abstractNote = {This study examined diel shifts in metabolic functions of Microcystis spp. during a 48-h Lagrangian survey of a toxin-producing cyanobacterial bloom in western Lake Erie in the aftermath of the 2014 Toledo Water Crisis. Transcripts mapped to the genomes of recently sequenced lower Great Lakes Microcystis isolates showed distinct patterns of gene expression between samples collected across day (10:00 h, 16:00 h) and night (22:00 h, 04:00 h). Daytime transcripts were enriched in functions related to Photosystem II (e.g., psbA), nitrogen and phosphate acquisition, cell division (ftsHZ), heat shock response (dnaK, groEL), and uptake of inorganic carbon (rbc, bicA). Genes transcribed during nighttime included those involved in phycobilisome protein synthesis and Photosystem I core subunits. Hierarchical clustering and principal component analysis (PCA) showed a tightly clustered group of nighttime expressed genes, whereas daytime transcripts were separated from each other over the 48-h duration. Lack of uniform clustering within the daytime transcripts suggested that the partitioning of gene expression in Microcystis is dependent on both circadian regulation and physicochemical changes within the environment.},
doi = {10.3389/fmicb.2019.02081},
journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = ,
volume = 10,
place = {United States},
year = {2019},
month = {9}
}

Works referenced in this record:

IMG/M 4 version of the integrated metagenome comparative analysis system
journal, October 2013

  • Markowitz, Victor M.; Chen, I-Min A.; Chu, Ken
  • Nucleic Acids Research, Vol. 42, Issue D1, p. D568-D573
  • DOI: 10.1093/nar/gkt919