Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations

Kim, Sunghoon; Zeng, Weizhong; Bernard, Shane; Liao, Jun; Venkateshwaran, Muthusubramanian; Ane, Jean-Michel; Jiang, Youxing

doi:10.1038/s41467-019-11698-5

Title: Ca²⁺-regulated Ca²⁺ channels with an RCK gating ring control plant symbiotic associations

Journal Article · Fri Aug 16 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-11698-5· OSTI ID:1570742

^[1]; Zeng, Weizhong ^[1]; Bernard, Shane ^[2];

^[3]; Venkateshwaran, Muthusubramanian ^[4]; Ane, Jean-Michel ^[2];

^[1]

Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
Univ. of Wisconsin, Madison, WI (United States)
ShanghaiTech Univ. (China)
University of Wisconsin, Platteville, WI (United States)

A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca²⁺ spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca²⁺ channel whose activation requires cytosolic/nucleosolic Ca²⁺, contrary to the previous suggestion of it being a K⁺ channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K⁺) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca²⁺-gated K⁺ (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca²⁺ sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca²⁺ binding sites to the physiological function of CASTOR as well as DMI1.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; AC02-05CH11231

OSTI ID:: 1570742

Journal Information:: Nature Communications, Vol. 10, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 23 works

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Title: Ca²⁺-regulated Ca²⁺ channels with an RCK gating ring control plant symbiotic associations