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Title: Drying and Rainfall Shape the Structure and Functioning of Nitrifying Microbial Communities in Riverbed Sediments

Abstract

Non-flow periods in fluvial ecosystems are a global phenomenon. Streambed drying and rewetting by sporadic rainfalls could drive considerable changes in the microbial communities that govern stream nitrogen (N) availability at different temporal and spatial scales. We performed a microcosm-based experiment to investigate how dry period duration (DPD) (0, 3, 6, and 9 weeks) and magnitude of sporadic rewetting by rainfall (0, 4, and 21 mm applied at end of dry period) affected stocks of N in riverbed sediments, ammonia-oxidizing bacteria (AOB) and archaea (AOA) and rates of ammonia oxidation (AO), and emissions of nitrous oxide (N 2O) to the atmosphere. While ammonium (NH 4 +) pool size decreased, nitrate (NO 3 -) pool size increased in sediments with progressive drying. Concomitantly, the relative and absolute abundance of AOB and, especially, AOA (assessed by 16S rRNA gene sequencing and quantitative PCR of ammonia monooxygenase genes) increased, despite an apparent decrease of AO rates with drying. An increase of N 2O emissions occurred at early drying before substantially dropping until the end of the experiment. Strong rainfall of 21 mm increased AO rates and NH 4 + in sediments, whereas modest rainfall of 4 mm triggered a notable increase of Nmore » 2O fluxes. Interestingly, such responses were detected only after 6 and 9 weeks of drying. Our results demonstrate that progressive drying drives considerable changes in in-stream N cycling and the associated nitrifying microbial communities, and that sporadic rainfall can modulate these effects. Our findings are particularly relevant for N processing and transport in rivers with alternating dry and wet phases – A hydrological scenario expected to become more important in the future.« less

Authors:
 [1];  [2];  [3];  [3];  [4];  [5];  [3];  [1]
  1. Leibniz-Inst. of Freshwater Ecology and Inland Fisheries (IGB), Berlin (Germany)
  2. Univ. of the Basque Country, Bilbao (Spain)
  3. Univ. of Greifswald (Germany)
  4. Humboldt Univ. of Berlin (Germany)
  5. Univ. of Vienna (Austria); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1567143
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal Issue: NOV; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; intermittent; stream; nitrification; ammonia oxidation; AOA; AOB; NOB; COMAMMOX

Citation Formats

Arce, Maria Isabel, von Schiller, Daniel, Bengtsson, Mia M., Hinze, Christian, Jung, Hoseung, Alves, Ricardo J. Eloy, Urich, Tim, and Singer, Gabriel. Drying and Rainfall Shape the Structure and Functioning of Nitrifying Microbial Communities in Riverbed Sediments. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.02794.
Arce, Maria Isabel, von Schiller, Daniel, Bengtsson, Mia M., Hinze, Christian, Jung, Hoseung, Alves, Ricardo J. Eloy, Urich, Tim, & Singer, Gabriel. Drying and Rainfall Shape the Structure and Functioning of Nitrifying Microbial Communities in Riverbed Sediments. United States. https://doi.org/10.3389/fmicb.2018.02794
Arce, Maria Isabel, von Schiller, Daniel, Bengtsson, Mia M., Hinze, Christian, Jung, Hoseung, Alves, Ricardo J. Eloy, Urich, Tim, and Singer, Gabriel. Fri . "Drying and Rainfall Shape the Structure and Functioning of Nitrifying Microbial Communities in Riverbed Sediments". United States. https://doi.org/10.3389/fmicb.2018.02794. https://www.osti.gov/servlets/purl/1567143.
@article{osti_1567143,
title = {Drying and Rainfall Shape the Structure and Functioning of Nitrifying Microbial Communities in Riverbed Sediments},
author = {Arce, Maria Isabel and von Schiller, Daniel and Bengtsson, Mia M. and Hinze, Christian and Jung, Hoseung and Alves, Ricardo J. Eloy and Urich, Tim and Singer, Gabriel},
abstractNote = {Non-flow periods in fluvial ecosystems are a global phenomenon. Streambed drying and rewetting by sporadic rainfalls could drive considerable changes in the microbial communities that govern stream nitrogen (N) availability at different temporal and spatial scales. We performed a microcosm-based experiment to investigate how dry period duration (DPD) (0, 3, 6, and 9 weeks) and magnitude of sporadic rewetting by rainfall (0, 4, and 21 mm applied at end of dry period) affected stocks of N in riverbed sediments, ammonia-oxidizing bacteria (AOB) and archaea (AOA) and rates of ammonia oxidation (AO), and emissions of nitrous oxide (N2O) to the atmosphere. While ammonium (NH4+) pool size decreased, nitrate (NO3-) pool size increased in sediments with progressive drying. Concomitantly, the relative and absolute abundance of AOB and, especially, AOA (assessed by 16S rRNA gene sequencing and quantitative PCR of ammonia monooxygenase genes) increased, despite an apparent decrease of AO rates with drying. An increase of N2O emissions occurred at early drying before substantially dropping until the end of the experiment. Strong rainfall of 21 mm increased AO rates and NH4+ in sediments, whereas modest rainfall of 4 mm triggered a notable increase of N2O fluxes. Interestingly, such responses were detected only after 6 and 9 weeks of drying. Our results demonstrate that progressive drying drives considerable changes in in-stream N cycling and the associated nitrifying microbial communities, and that sporadic rainfall can modulate these effects. Our findings are particularly relevant for N processing and transport in rivers with alternating dry and wet phases – A hydrological scenario expected to become more important in the future.},
doi = {10.3389/fmicb.2018.02794},
url = {https://www.osti.gov/biblio/1567143}, journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = NOV,
volume = 9,
place = {United States},
year = {2018},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
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Citation Metrics:
Cited by: 8 works
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Figures / Tables:

FIGURE 1 FIGURE 1: Mean (±SE, n = 9) WC and DO concentration in surface and deep sediments monitored over the entire experiment in dry microcosms with no rainfall application ( A). Mean WC (± SE, n = 3) in surface ( B) and deep ( C) sediments during initial conditions (0more » weeks) and after the different RMs for each DPD. Asterisks (*) denote significant ( P ≤ 0.05) differences to initial wet conditions after Student’s t-test followed by Holm’s correction for multiple comparisons. The dashed line represents the initial conditions as a reference for a better comparison.« less

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    Works referencing / citing this record:

    Connectivity and Nitrate Uptake Potential of Intermittent Streams in the Northeast USA
    journal, June 2019


    Vegetation-Dependent Response to Drought in Salt Marsh Ammonia-Oxidizer Communities
    journal, December 2019


      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.