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Title: Neutron and X-ray analysis of the Fenna–Matthews–Olson photosynthetic antenna complex from Prosthecochloris aestuarii

Abstract

The Fenna–Matthews–Olson protein fromProsthecochloris aestuarii(PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space groupH3, with unit-cell parametersa=b= 83.64,c= 294.78 Å, and diffracted X-rays to ∼1.7 Å resolution at room temperature. LargePaFMO crystals grown to volumes of 0.3–0.5 mm 3diffracted neutrons to 2.2 Å resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.

Authors:
; ; ; ORCiD logo; ORCiD logo; ; ;
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC); Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1566705
DOE Contract Number:  
SC0001035
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F, Structural Biology Communications
Additional Journal Information:
Journal Volume: 75; Journal Issue: 3; Journal ID: ISSN 2053-230X
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly)

Citation Formats

Lu, Xun, Selvaraj, Brinda, Ghimire-Rijal, Sudipa, Orf, Gregory S., Meilleur, Flora, Blankenship, Robert E., Cuneo, Matthew J., and Myles, Dean A. A. Neutron and X-ray analysis of the Fenna–Matthews–Olson photosynthetic antenna complex from Prosthecochloris aestuarii. United States: N. p., 2019. Web. doi:10.1107/s2053230x19000724.
Lu, Xun, Selvaraj, Brinda, Ghimire-Rijal, Sudipa, Orf, Gregory S., Meilleur, Flora, Blankenship, Robert E., Cuneo, Matthew J., & Myles, Dean A. A. Neutron and X-ray analysis of the Fenna–Matthews–Olson photosynthetic antenna complex from Prosthecochloris aestuarii. United States. doi:10.1107/s2053230x19000724.
Lu, Xun, Selvaraj, Brinda, Ghimire-Rijal, Sudipa, Orf, Gregory S., Meilleur, Flora, Blankenship, Robert E., Cuneo, Matthew J., and Myles, Dean A. A. Wed . "Neutron and X-ray analysis of the Fenna–Matthews–Olson photosynthetic antenna complex from Prosthecochloris aestuarii". United States. doi:10.1107/s2053230x19000724.
@article{osti_1566705,
title = {Neutron and X-ray analysis of the Fenna–Matthews–Olson photosynthetic antenna complex from Prosthecochloris aestuarii},
author = {Lu, Xun and Selvaraj, Brinda and Ghimire-Rijal, Sudipa and Orf, Gregory S. and Meilleur, Flora and Blankenship, Robert E. and Cuneo, Matthew J. and Myles, Dean A. A.},
abstractNote = {The Fenna–Matthews–Olson protein fromProsthecochloris aestuarii(PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space groupH3, with unit-cell parametersa=b= 83.64,c= 294.78 Å, and diffracted X-rays to ∼1.7 Å resolution at room temperature. LargePaFMO crystals grown to volumes of 0.3–0.5 mm3diffracted neutrons to 2.2 Å resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.},
doi = {10.1107/s2053230x19000724},
journal = {Acta Crystallographica. Section F, Structural Biology Communications},
issn = {2053-230X},
number = 3,
volume = 75,
place = {United States},
year = {2019},
month = {2}
}

Works referenced in this record:

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925