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Title: Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium

Abstract

Tolyporphins are structurally diverse tetrapyrrole macrocycles produced by the cyanobacterial culture HT-58-2. Although tolyporphins were discovered over 25 years ago, little was known about the microbiology of the culture. The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT-58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. HT-58-2, for which the complete genome is established and growth properties are examined. Fluorescence in situ hybridization (FISH) analysis of the cyanobacterium–microbial community with a probe targeting the 16S rRNA of Porphyrobacter sp. HT-58-2 showed fluorescence emanating from the cyanobacterial sheath. Although genes for the biosynthesis of bacteriochlorophyll a (BChl a) are present in the Porphyrobacter sp. HT-58-2 genome, the pigment was not detected under the conditions examined, implying the absence of phototrophic growth. Comparative analysis of four Porphyrobacter spp. genomes from worldwide collection sites showed significant collinear gene blocks, with two inversions and three deletion regions. Taken together, the results enrich our understanding of the HT-58-2 cyanobacterium–microbial culture.

Authors:
 [1];  [2];  [2];  [2];  [3];  [4];  [2];  [5]
  1. 1​Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA; 2​Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina 27695-7615, USA
  2. 1​Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA
  3. 1​Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA; 3​Enloe Magnet High School, Raleigh, North Carolina 27610, USA
  4. 4​Department of Chemistry, University of Hawaii at Manoa, Honolulu, Hawaii 96822-2275, USA
  5. 2​Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina 27695-7615, USA
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC); Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1566677
DOE Contract Number:  
SC0001035
Resource Type:
Journal Article
Journal Name:
Microbiology
Additional Journal Information:
Journal Volume: 164; Journal Issue: 10; Journal ID: ISSN 1350-0872
Publisher:
Microbiology Society
Country of Publication:
United States
Language:
English
Subject:
solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly)

Citation Formats

Hughes, Rebecca-Ayme, Jin, Xiaohe, Zhang, Yunlong, Zhang, Ran, Tran, Sabrina, Williams, Philip G., Lindsey, Jonathan S., and Miller, Eric S. Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium. United States: N. p., 2018. Web. doi:10.1099/mic.0.000706.
Hughes, Rebecca-Ayme, Jin, Xiaohe, Zhang, Yunlong, Zhang, Ran, Tran, Sabrina, Williams, Philip G., Lindsey, Jonathan S., & Miller, Eric S. Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium. United States. doi:10.1099/mic.0.000706.
Hughes, Rebecca-Ayme, Jin, Xiaohe, Zhang, Yunlong, Zhang, Ran, Tran, Sabrina, Williams, Philip G., Lindsey, Jonathan S., and Miller, Eric S. Mon . "Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium". United States. doi:10.1099/mic.0.000706.
@article{osti_1566677,
title = {Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium},
author = {Hughes, Rebecca-Ayme and Jin, Xiaohe and Zhang, Yunlong and Zhang, Ran and Tran, Sabrina and Williams, Philip G. and Lindsey, Jonathan S. and Miller, Eric S.},
abstractNote = {Tolyporphins are structurally diverse tetrapyrrole macrocycles produced by the cyanobacterial culture HT-58-2. Although tolyporphins were discovered over 25 years ago, little was known about the microbiology of the culture. The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT-58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. HT-58-2, for which the complete genome is established and growth properties are examined. Fluorescence in situ hybridization (FISH) analysis of the cyanobacterium–microbial community with a probe targeting the 16S rRNA of Porphyrobacter sp. HT-58-2 showed fluorescence emanating from the cyanobacterial sheath. Although genes for the biosynthesis of bacteriochlorophyll a (BChl a) are present in the Porphyrobacter sp. HT-58-2 genome, the pigment was not detected under the conditions examined, implying the absence of phototrophic growth. Comparative analysis of four Porphyrobacter spp. genomes from worldwide collection sites showed significant collinear gene blocks, with two inversions and three deletion regions. Taken together, the results enrich our understanding of the HT-58-2 cyanobacterium–microbial culture.},
doi = {10.1099/mic.0.000706},
journal = {Microbiology},
issn = {1350-0872},
number = 10,
volume = 164,
place = {United States},
year = {2018},
month = {10}
}

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