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Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium

Journal Article · · Microbiology
DOI:https://doi.org/10.1099/mic.0.000706· OSTI ID:1566677
 [1];  [2];  [2];  [2];  [3];  [4];  [2];  [5]
  1. 1​Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA; 2​Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina 27695-7615, USA
  2. 1​Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA
  3. 1​Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA; 3​Enloe Magnet High School, Raleigh, North Carolina 27610, USA
  4. 4​Department of Chemistry, University of Hawaii at Manoa, Honolulu, Hawaii 96822-2275, USA
  5. 2​Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina 27695-7615, USA
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Tolyporphins are structurally diverse tetrapyrrole macrocycles produced by the cyanobacterial culture HT-58-2. Although tolyporphins were discovered over 25 years ago, little was known about the microbiology of the culture. The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT-58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. HT-58-2, for which the complete genome is established and growth properties are examined. Fluorescence in situ hybridization (FISH) analysis of the cyanobacterium–microbial community with a probe targeting the 16S rRNA of Porphyrobacter sp. HT-58-2 showed fluorescence emanating from the cyanobacterial sheath. Although genes for the biosynthesis of bacteriochlorophyll a (BChl a) are present in the Porphyrobacter sp. HT-58-2 genome, the pigment was not detected under the conditions examined, implying the absence of phototrophic growth. Comparative analysis of four Porphyrobacter spp. genomes from worldwide collection sites showed significant collinear gene blocks, with two inversions and three deletion regions. Taken together, the results enrich our understanding of the HT-58-2 cyanobacterium–microbial culture.

Research Organization:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC); Washington Univ., St. Louis, MO (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
DOE Contract Number:
SC0001035
OSTI ID:
1566677
Journal Information:
Microbiology, Vol. 164, Issue 10; ISSN 1350-0872
Publisher:
Microbiology Society
Country of Publication:
United States
Language:
English

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