Detection of lipopolysaccharides in serum using a waveguide-based optical biosensor

Journal Article · Fri Feb 17 00:00:00 EST 2017 · Proceedings of SPIE - The International Society for Optical Engineering

DOI:https://doi.org/10.1117/12.2253506· OSTI ID:1565840

Noormohamed, Aneesa Abdullatif ^[1]; Stromberg, Loreen R. ^[2]; Anderson, Aaron S.  ^[1]; Karim, Zachary ^[2]; Dighe, Priya Kalpak  ^[1]; Kempaiah, Prakasha ^[2]; Ong'echa, John M. ^[3]; Perkins, Douglas J. ^[2]; Doggett, Norman Alan  ^[1]; Mcmahon, Benjamin Hamilton  ^[1]; Mukundan, Harshini  ^[1]

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1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
2. Univ. of New Mexico, Albuquerque, NM (United States)
3. Univ. of New Mexico, Albuquerque, NM (United States); Kenya Medical Research Institute (Kenya)

We report that direct ultra-sensitive detection of pathogen biomarkers in blood could provide a universal strategy for diagnosis of bacterial infections, which remain a leading cause of morbidity and mortality in many areas of the world. Many factors complicate diagnosis, including the presence of multiple co-infections in a given patient, and lack of infrastructure in rural settings. In some pediatric patients, such as those in areas with poor resources, an additional challenge exists with low sample volumes due to age and other health factors such as anemia and dehydration. Our team is working on developing novel diagnostic assays, with a waveguide-based biosensor platform, to rapidly and specifically identify pathogen biomarkers from small samples of serum or plasma, allowing for the timely and sensitive diagnosis of infection at the point of care. In addition to the platform, we have developed novel membrane insertion and lipoprotein capture assay methods, to capture lipidated pathogen biomarkers in aqueous blood, by virtue of their interactions with host lipoprotein carriers. In this study, we demonstrate our efforts to adapt the lipoprotein capture assay for the detection of small concentrations of pathogen-secreted lipopolysaccharides in aqueous blood, with the ultimate aim of diagnosing Gram-negative infections effectively.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE Laboratory Directed Research and Development (LDRD) Program
• Grant/Contract Number: 89233218CNA000001
• OSTI ID: 1565840
• Report Number(s): LA-UR-17-20261
• Journal Information: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 10072; Conference: SPIE BiOS , San Francisco, CA (United States), 28 Jan - 2 Feb 2017; ISSN 0277-786X
• Publisher: SPIE
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES